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Human TNF RI/TNFRSF1A Quantikine ELISA Kit

Catalog #: DRT100
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QC05: Quantikine Immunoassay Control Group 2
Human TNF RI/TNFRSF1A ELISA Calibrator Diluent RD5-5 Standard Curve
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Summary Sample Values Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human TNF RI/TNFRSF1A Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (200 uL), Serum (20 uL), EDTA Plasma (20 uL), Heparin Plasma (20 uL), Citrate Plasma (20 uL), Urine (20 uL)
Sensitivity
1.2 pg/mL
Assay Range
7.8 - 500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine)
Specificity
Natural and recombinant human sTNF RI
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.

Sample Values

Serum/Plasma/Urine - Samples from apparently healthy volunteers were evaluated for the presence of human TNF RI in this assay. No medical histories were available for the donors used in this study.

Sample TypeMean (pg/mL)Range (pg/mL)Standard Deviation (pg/mL)
Serum (n=40)1198749-1966256
EDTA plasma (n=40)914484-1407208
Heparin plasma (n=40)1015512-1739245
Citrate plasma (n=40)856488-1598210
Urine* (n=33)1029173-4030832
*Values are actual and not normalized for creatinine content.

Cell Culture Supernates - Human peripheral blood mononuclear cells (1 x 106 cells/mL) were cultured in RPMI supplemented with 10% fetal bovine serum, 50 μM beta -mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. Cells were stimulated with 10 μg/mL PHA, 10 μg/mL PHA + 10 ng/mL recombinant human (rh) IL-2, 50 ng/mL PMA, or 50 ng/mL LPS. Aliquots of the cell culture supernates were removed on days 1, 3, and 5 and assayed for levels of human TNF RI.

StimulantDay 1 (pg/mL)Day 3 (pg/mL)Day 5 (pg/mL)
PHA2476141
PHA + rhIL-22671143
PMA162767
LPS17 3649






Product Summary

The Quantikine Human sTNF RI Immunoassay is a 4.5 hour solid phase ELISA designed to measure sTNF RI in cell culture supernates, serum, plasma, and urine. It contains E. coli-expressed recombinant human sTNF RI, as well as antibodies raised against this polypeptide. The recombinant protein represents the non-glycosylated, N-terminal methionyl form of the naturally occurring human soluble Type I receptor for TNF with an apparent molecular weight of approximately 18.6 kDa. This immunoassay has been shown to accurately quantitate the recombinant sTNF RI. Results obtained on samples containing natural sTNF RI showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values of natural sTNF RI. Since the measurement of human sTNF RI by this immunoassay is relatively insensitive to added TNF-alpha or TNF-beta, it is probable that this measurement corresponds to the total amount of the soluble receptor present in samples (i.e. the total amount of free receptor plus the total amount of receptor bound to TNF).

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 41.5 122 231 54 251 350
Standard Deviation 2.14 5.38 11 2.7 9.7 23.3
CV% 5.2 4.4 4.8 5 3.9 6.7

Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 69 198 355 54.8 252 356
Standard Deviation 3.24 7.17 17.8 4.8 9.3 20.6
CV% 4.7 3.6 5 8.8 3.7 5.8

Recovery

The recovery of sTNF RI spiked to three levels in samples throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=8) 85 80-92
EDTA Plasma (n=8) 86 70-97
Heparin Plasma (n=8) 93 79-103
Serum (n=8) 90 77-103
Urine (n=8) 85 71-109

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of sTNF RI were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human TNF RI/TNFRSF1A ELISA Linearity

Scientific Data

N/A Human TNF RI/TNFRSF1A ELISA Calibrator Diluent RD5-5 Standard Curve View Larger

Human TNF RI/TNFRSF1A ELISA Calibrator Diluent RD5-5 Standard Curve

N/A Human TNF RI/TNFRSF1A ELISA Calibrator Diluent RD6O Standard Curve View Larger

Human TNF RI/TNFRSF1A ELISA Calibrator Diluent RD6O Standard Curve

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TNF RI/TNFRSF1A

Tumor necrosis factors (TNFs) are pleiotropic cytokines that are considered primary modifiers of the inflammatory and immune reactions of animals produced in response to injury or infection. Two forms of TNF, designated TNF-alpha (or cachectin) and TNF-beta (or lymphotoxin), have been described that share 30% sequence similarity and compete for binding to the same receptors. TNFs play a necessary and beneficial role as mediators of host resistance to infections and tumor formation. However, over-production or inappropriate expression of these factors can lead to a variety of pathological conditions, including wasting, systemic toxicity, and septic shock. For reviews of the literature relating to these factors, see references 1 and 2. 

The actions of TNFs are produced subsequent to binding of the factors to cell surface receptors. Two distinct TNF receptors have been identified and cloned. Virtually all cell types studied show the presence of one or both of these receptor types. One receptor type, termed TNF RII (Type A, Type a, 75 kDa or utr antigen), shows an apparent molecular weight of 75 kDa. The gene for this receptor encodes a presumptive transmembrane protein of 439 amino acid (aa) residues (3, 19). The other receptor type, termed TNF RI (Type B, Type b, 55 kDa or htr antigen), shows an apparent molecular weight of 55 kDa. The gene for this protein encodes a transmembrane protein of 426 aa residues (4, 5, 19). Both receptor types show high affinity binding of either TNF-alpha or TNF-beta. The two receptor types are immunologically distinct but their extracellular domains show similarities in the pattern of cysteine residue locations in four domains (3). The intracellular domains of the two receptor types are apparently unrelated, suggesting the possibility that the two receptor types employ different signal transduction pathways. 
Several groups have identified soluble TNF binding proteins in human serum and urine (6-8) that can neutralize the biological activities of TNF-alpha and TNF-beta. Two types have been identified and designated sTNF RI (or TNF BPI) and sTNF RII (or TNF BPII). These soluble forms have now been shown to represent truncated forms of the two types of TNF receptors discussed above. The soluble receptor forms apparently arise as a result of shedding of the extracellular domains of the receptors, and concentrations of about 1-2 ng/mL are found in the serum and urine of healthy subjects (9, 10). The levels of the soluble receptors vary from individual to individual but are stable over time for given individuals (9). 
Elevated levels of TNF receptors have been found in the amniotic fluid and urine of pregnant women (11), in serum or plasma in association with pathological conditions such as endotoxinemia (12, 13), meningiococcemia (14), and HIV infection (15), and in plasma and ascites of patients in association with infections and malignancies (16). The mechanisms involved in the induction of shedding of the TNF receptors are not well understood. There are reports of correlations between increased TNF levels and soluble receptor levels, suggesting generally that stimuli that cause TNF levels to rise also induce shedding of TNF receptors (12-14, 17). There is also evidence, however, that suggests the shedding of the two types of soluble receptors is independently regulated (13). 2 For research use only. Not for use in diagnostic procedures. 
The physiological role of the soluble TNF receptors is not known. It is known that both types of soluble receptors can bind to TNF in vitro and inhibit its biological activity by competing with cell surface receptors for TNF binding. Consequently it has been suggested that shedding of soluble receptors in response to TNF release could serve as a mechanism for binding and inhibiting the TNF not immediately bound to surface receptors, thus protecting other cells from the effects of TNF and localizing the inflammatory response (12, 17). It is also possible that shedding of receptors represents a mechanism for desensitizing the cells that shed the receptors from the effects of TNF (17). On the other hand, it has been reported that at low concentrations of TNF, binding to soluble receptors can stabilize TNF and augment some of its activities (18). Thus it is possible that under some conditions the pool of TNF bound to soluble receptors could represent a reservoir for the stabilization and controlled release of TNF

Long Name:
Tumor Necrosis Factor Receptor I
Entrez Gene IDs:
7132 (Human); 21937 (Mouse); 25625 (Rat); 102135285 (Cynomolgus Monkey)
Alternate Names:
CD120a antigen; CD120a; FPF; p55; p55-R; p60; TNF RI; TNFARMGC19588; TNF-R; TNF-R1; TNFR1TBP1; TNFR55; TNF-R55; TNFR60; TNFRI; TNF-RI; TNF-R-I; TNFR-I; TNFRSF1A; tumor necrosis factor binding protein 1; Tumor necrosis factor receptor 1; tumor necrosis factor receptor 1A isoform beta; tumor necrosis factor receptor superfamily member 1A; tumor necrosis factor receptor superfamily, member 1A; tumor necrosis factor receptor type 1; Tumor necrosis factor receptor type I; tumor necrosis factor-alpha receptor
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 50 µL Assay Diluent
  3.   Add 50 µL of Assay Diluent to each well.
    4. 200 µL Standard, Control, or Sample
  4.   Add 200 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  5.   Aspirate each well and wash, repeating the process twice for a total of 3 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate and wash 3 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 20 minutes. PROTECT FROM LIGHT.
    9. 50 µL Stop Solution
  9. Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human TNF RI/TNFRSF1A Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

86 Citations: Showing 1 - 10
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  1. Intestinal microbiota composition is predictive of radiotherapy-induced acute gastrointestinal toxicity in prostate cancer patients
    Authors: Iacovacci, J;Serafini, MS;Avuzzi, B;Badenchini, F;Cicchetti, A;Devecchi, A;Dispinzieri, M;Doldi, V;Giandini, T;Gioscio, E;Mancinelli, E;Noris Chiorda, B;Orlandi, E;Palorini, F;Possenti, L;Reis Ferreira, M;Villa, S;Zaffaroni, N;De Cecco, L;Valdagni, R;Rancati, T;
    EBioMedicine
    Species: Human
    Sample Types: Plasma
  2. Correlation of Increased Soluble Tumor Necrosis Factor Receptor 1 with Mortality and Dependence on Treatment in Non-Small-Cell Lung Cancer Patients: A Longitudinal Cohort Study
    Authors: Hassan, L;Bedir, A;Kraus, FB;Ostheimer, C;Vordermark, D;Mikolajczyk, R;Seliger, B;Medenwald, D;
    Cancers
    Species: Human
    Sample Types: Serum
  3. Hemophagocytic Lymphohistiocytosis With Elevated Cytokines Related to Macrophage Activation After Liver Transplantation for Autoimmune Hepatitis: A Case Report
    Authors: Nakanuma, S;Gabata, R;Okazaki, M;Seki, A;Hosokawa, K;Yokoyama, T;Katano, K;Sugita, H;Tokoro, T;Takada, S;Makino, I;Taniguchi, T;Harada, K;Yagi, S;
    Transplantation proceedings
    Species: Human
    Sample Types: Serum
  4. High molar ratios of tumor necrosis factor ? (TNF ?) soluble receptors I and II to the TNF ligand in human plasma from male veterans with comorbid posttraumatic stress disorder (PTSD) and mild traumatic brain injury (mTBI)
    Authors: Morales, TI;Stamper, CE;Brenner, LA;
    The European journal of psychiatry
    Species: Human
    Sample Types: Plasma
  5. Elevated Soluble TNF-Receptor 1 in the Serum of Predementia Subjects with Cerebral Small Vessel Disease
    Authors: KHT Salai, LY Wu, JR Chong, YL Chai, B Gyanwali, C Robert, S Hilal, N Venketasub, GS Dawe, CP Chen, MKP Lai
    Biomolecules, 2023-03-13;13(3):.
    Species: Human
    Sample Types: Serum
  6. Circulating tumor necrosis factor receptors are associated with mortality and disease severity in COVID-19 patients
    Authors: T Gohda, M Murakoshi, Y Suzuki, M Hiki, T Naito, K Takahashi, Y Tabe
    PLoS ONE, 2022-10-11;17(10):e0275745.
    Species: Human
    Sample Types: Serum
  7. Improved prediction of radiation pneumonitis by combining biological and radiobiological parameters using a data-driven Bayesian network analysis
    Authors: T Hinton, D Karnak, M Tang, R Jiang, Y Luo, P Boonstra, Y Sun, DJ Nancarrow, E Sandford, P Ray, C Maurino, M Matuszak, MJ Schipper, MD Green, GA Yanik, M Tewari, IE Naqa, CA Schonewolf, RT Haken, S Jolly, TS Lawrence, D Ray
    Translational Oncology, 2022-04-20;21(0):101428.
    Species: Human
    Sample Types: Plasma
  8. Inflammatory Markers and Risk of Heart Failure With Reduced to Preserved Ejection Fraction
    Authors: Z Albar, M Albakri, J Hajjari, M Karnib, SE Janus, SG Al-Kindi
    The American journal of cardiology, 2022-01-03;0(0):.
    Species: Human
    Sample Types: Serum
  9. Synergistic association of the copper/zinc ratio under inflammatory conditions with diabetic kidney disease in patients with type 2 diabetes: The Asahi Diabetes Complications Study
    Authors: T Takao, H Yanagisawa, M Suka, Y Yoshida, Y Onishi, T Tahara, T Kikuchi, A Kushiyama, M Anai, K Takahashi, S Wakabayash, H Yamazaki, S Kawazu, Y Iwamoto, M Noda, M Kasuga
    Journal of diabetes investigation, 2021-10-07;0(0):.
    Species: Human
    Sample Types: Serum
  10. Circulating miRNA expression in asthmatics is age-related and associated with clinical asthma parameters, respiratory function and systemic inflammation
    Authors: A Wardzy?ska, M Pawe?czyk, J Rywaniak, J Makowska, J Jamroz-Brz, ML Kowalski
    Respiratory Research, 2021-06-10;22(1):177.
    Species: Human
    Sample Types: Serum
  11. TNFR1 and the TNF&alpha axis as a targetable mediator of liver injury from stereotactic body radiation therapy
    Authors: MM Cousins, E Morris, C Maurino, TP Devasia, D Karnak, D Ray, ND Parikh, D Owen, RK Ten Haken, MJ Schipper, TS Lawrence, KC Cuneo
    Translational Oncology, 2020-12-13;14(1):100950.
    Species: Human
    Sample Types: Plasma
  12. Identification of distinct immune activation profiles in adult humans
    Authors: R Cezar, A Winter, D Desigaud, M Pastore, L Kundura, AM Dupuy, C Cognot, T Vincent, C Reynes, C Dunyach-Re, JP Lavigne, R Sabatier, P Le Merre, E Maggia, P Corbeau
    Scientific Reports, 2020-11-30;10(1):20824.
    Species: Human
    Sample Types: Plasma
  13. Comparison of Circulating Biomarkers in Predicting Diabetic Kidney Disease Progression With Autoantibodies to Erythropoietin Receptor
    Authors: M Oshima, A Hara, T Toyama, M Jun, C Pollock, M Jardine, S Harrap, N Poulter, ME Cooper, M Woodward, J Chalmers, V Perkovic, MG Wong, T Wada
    Kidney international reports, 2020-11-10;6(2):284-295.
    Species: Human
    Sample Types: Plasma
  14. The association between change of soluble tumor necrosis factor receptor R1 (sTNF-R1) measurements and cardiovascular and all-cause mortality-Results from the population-based (Cardiovascular Disease, Living and Ageing in Halle) CARLA study 2002-2016
    Authors: L Hassan, D Medenwald, D Tiller, A Kluttig, B Ludwig-Kra, FB Kraus, KH Greiser, R Mikolajczy
    PLoS ONE, 2020-10-26;15(10):e0241213.
    Species: Human
    Sample Types: Serum
  15. The transition to family caregiving and its effect on biomarkers of inflammation
    Authors: DL Roth, WE Haley, OC Sheehan, J Huang, JD Rhodes, P Durda, VJ Howard, JD Walston, M Cushman
    Proc. Natl. Acad. Sci. U.S.A., 2020-06-24;0(0):.
    Species: Human
    Sample Types: Serum
  16. Circulating Tumor Necrosis Factor Receptors: A Potential Biomarker for the Progression of Diabetic Kidney Disease
    Authors: M Murakoshi, T Gohda, Y Suzuki
    Int J Mol Sci, 2020-03-13;21(6):.
    Species: Human
    Sample Types: Plasma
  17. Factor Xa Inhibition Reduces Coagulation Activity but Not Inflammation Among People With HIV: A Randomized Clinical Trial
    Authors: JV Baker, J Wolfson, T Peterson, M Mooberry, M Gissel, H Mystakelis, MW Henderson, K Garcia-Mye, FS Rhame, TW Schacker, KE Brummel-Zi, I Sereti, NS Key, RP Tracy
    Open Forum Infect Dis, 2020-02-01;7(2):ofaa026.
    Species: Human
    Sample Types: Serum
  18. Pretransplant Levels of C-Reactive Protein,�Soluble TNF Receptor-1, and�CD38+HLADR+ CD8 T Cells Predict Risk of Allograft Rejection in HIV+ Kidney Transplant Recipients
    Authors: JF Camargo, S Pallikkuth, I Moroz, Y Natori, ML Alcaide, A Rodriguez, G Guerra, GW Burke, S Pahwa
    Kidney Int Rep, 2019-08-19;4(12):1705-1716.
    Species: Human
    Sample Types: Plasma
  19. Store-operated calcium entry (SOCE) contributes to phosphorylation of p38 MAPK and suppression of TNF-α signalling in the intestinal epithelial cells
    Authors: J Uwada, T Yazawa, H Nakazawa, D Mikami, SM Krug, M Fromm, K Sada, I Muramatsu, T Taniguchi
    Cell. Signal., 2019-07-08;63(0):109358.
    Species: Human
    Sample Types: Cell Culture Supernates
  20. Systemic Inflammation Precedes Microalbuminuria in Diabetes
    Authors: FG Scurt, J Menne, S Brandt, A Bernhardt, PR Mertens, H Haller, C Chatzikyrk
    Kidney Int Rep, 2019-06-21;4(10):1373-1386.
    Species: Human
    Sample Types: Serum
  21. Serum soluble ST2 as a marker of renal scar in pediatric upper urinary tract infection
    Authors: N Ohta, H Yasudo, M Mizutani, T Matsushige, R Fukano, S Kittaka, K Maehara, K Ichihara, S Ohga, S Hasegawa
    Cytokine, 2019-05-29;120(0):258-263.
    Species: Human
    Sample Types: Serum
  22. Inflammatory biomarkers, geriatric assessment, and treatment outcomes in acute myeloid leukemia
    Authors: KP Loh, JA Tooze, BJ Nicklas, SB Kritchevsk, JD Williamson, LR Ellis, BL Powell, TS Pardee, NG Goyal, HD Klepin
    J Geriatr Oncol, 2019-04-05;0(0):.
    Species: Human
    Sample Types: Plasma
  23. Tumor necrosis factor-alpha and Fas/Fas ligand signaling pathways in chronic spontaneous urticaria
    Authors: R Grzanka, A Damasiewic, A Kasperska-
    Allergy Asthma Clin Immunol, 2019-03-14;15(0):15.
    Species: Human
    Sample Types: Serum
  24. Infliximab Pharmacokinetics are Influenced by Intravenous Immunoglobulin Administration in Patients with Kawasaki Disease
    Authors: N Vande Cast, J Oyamada, C Shimizu, BM Best, EV Capparelli, AH Tremoulet, JC Burns
    Clin Pharmacokinet, 2018-12-01;0(0):.
    Species: Human
    Sample Types: Plasma
  25. Elevated Inflammatory Plasma Biomarkers in Patients With Fabry Disease: A Critical Link to Heart Failure With Preserved Ejection Fraction
    Authors: H Yogasundar, A Nikhanj, BN Putko, M Boutin, S Jain-Ghai, A Khan, C Auray-Blai, ML West, GY Oudit
    J Am Heart Assoc, 2018-11-06;7(21):e009098.
    Species: Human
    Sample Types: Plasma
  26. Biomarkers and immunoprofiles associated with fetal abnormalities of ZIKV-positive pregnancies
    Authors: SS Foo, W Chen, Y Chan, WS Lee, SA Lee, G Cheng, K Nielsen-Sa, P Brasil, JU Jung
    JCI Insight, 2018-11-02;3(21):.
    Species: Human
    Sample Types: Serum
  27. HIV-1 proviral landscapes distinguish posttreatment controllers from noncontrollers
    Authors: R Sharaf, GQ Lee, X Sun, B Etemad, LM Aboukhater, Z Hu, ZL Brumme, E Aga, RJ Bosch, Y Wen, G Namazi, C Gao, EP Acosta, RT Gandhi, JM Jacobson, D Skiest, DM Margolis, R Mitsuyasu, P Volberding, E Connick, DR Kuritzkes, MM Lederman, XG Yu, M Lichterfel, JZ Li
    J. Clin. Invest., 2018-08-20;0(0):.
    Species: Human
    Sample Types: Plasma
  28. Host Immunological Effects of Partial Splenic Embolization in Patients with Liver Cirrhosis
    Authors: Y Matsukiyo, H Nagai, T Matsui, Y Igarashi
    J Immunol Res, 2018-07-15;2018(0):1746391.
    Species: Human
    Sample Types: Serum
  29. ST6Gal-I sialyltransferase promotes tumor necrosis factor (TNF)-mediated cancer cell survival via sialylation of the TNF receptor 1 (TNFR1) death receptor
    Authors: AT Holdbrooks, CM Britain, SL Bellis
    J. Biol. Chem., 2017-12-12;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  30. Reevaluation of immune activation in the era of cART and an aging HIV-infected population
    Authors: LR de Armas, S Pallikkuth, V George, S Rinaldi, R Pahwa, KL Arheart, S Pahwa
    JCI Insight, 2017-10-19;2(20):.
    Species: Human
    Sample Types: Plasma
  31. Prospective Analysis of Lipid Composition Changes with Antiretroviral Therapy and Immune Activation in Persons Living with HIV
    Authors: MA Belury, E Bowman, J Gabriel, B Snyder, M Kulkarni, M Palettas, X Mo, JE Lake, D Zidar, SF Sieg, B Rodriguez, MP Playford, A Andrade, DR Kuritzkes, NN Mehta, MM Lederman, NT Funderburg
    Pathog Immun, 2017-10-06;2(3):376-403.
    Species: Human
    Sample Types: Plasma
  32. Distinctive inflammatory profile between acute focal bacterial nephritis and acute pyelonephritis in children
    Authors: M Mizutani, S Hasegawa, T Matsushige, N Ohta, S Kittaka, M Hoshide, T Kusuda, K Takahashi, K Ichihara, S Ohga
    Cytokine, 2017-07-03;99(0):24-29.
    Species: Human
    Sample Types: Serum
  33. Activation of muscarinic receptors prevents TNF-?-mediated intestinal epithelial barrier disruption through p38 MAPK
    Authors: J Uwada, T Yazawa, MT Islam, MRI Khan, SM Krug, M Fromm, SI Karaki, Y Suzuki, A Kuwahara, H Yoshiki, K Sada, Muramatsu, T Taniguchi
    Cell. Signal., 2017-04-12;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  34. Circulating TNF Receptors 1 and 2 Predict Mortality in Patients with End-stage Renal Disease Undergoing Dialysis
    Authors: T Gohda, S Maruyama, N Kamei, S Yamaguchi, T Shibata, M Murakoshi, S Horikoshi, Y Tomino, I Ohsawa, H Gotoh, S Nojiri, Y Suzuki
    Sci Rep, 2017-03-03;7(0):43520.
    Species: Human
    Sample Types: Serum
  35. Soluble Tumor Necrosis Factor Receptors and Arterial Stiffness in Patients With Coronary Atherosclerosis
    Authors: Hack-Lyoung Kim
    Am. J. Hypertens, 2017-03-01;0(0):.
    Species: Human
    Sample Types: Plasma
  36. Effects of an Encapsulated Fruit and Vegetable Juice Concentrate on Obesity-Induced Systemic Inflammation: A Randomised Controlled Trial
    Authors: EJ Williams, KJ Baines, BS Berthon, LG Wood
    Nutrients, 2017-02-08;9(2):.
    Species: Human
    Sample Types: Plasma
  37. Prevalent peripheral arterial disease and inflammatory burden
    BMC Geriatr, 2016-12-09;16(1):213.
    Species: Human
    Sample Types: Serum
  38. Anti-TNF-? Drugs Differently Affect the TNF?-sTNFR System and Monocyte Subsets in Patients with Psoriasis
    PLoS ONE, 2016-12-09;11(12):e0167757.
    Species: Human
    Sample Types: Plasma
  39. Altered Monocyte and Endothelial Cell Adhesion Molecule Expression Is Linked to Vascular Inflammation in Human Immunodeficiency Virus Infection
    Authors: M Kulkarni, E Bowman, J Gabriel, T Amburgy, E Mayne, DA Zidar, C Maierhofer, AN Turner, JA Bazan, SL Koletar, MM Lederman, SF Sieg, NT Funderburg
    Open Forum Infect Dis, 2016-10-15;3(4):ofw224.
    Species: Human
    Sample Types: Plasma
  40. Anti-Drug Antibodies, Drug Levels, Interleukin-6 and Soluble TNF Receptors in Rheumatoid Arthritis Patients during the First 6 Months of Treatment with Adalimumab or Infliximab: A Descriptive Cohort Study
    PLoS ONE, 2016-09-08;11(9):e0162316.
    Species: Human
    Sample Types: Serum
  41. Local TNF causes NFATc1-dependent cholesterol-mediated podocyte injury
    J Clin Invest, 2016-08-02;0(0):.
    Species: Human
    Sample Types: Serum
  42. Inflammatory Markers and the Risk of Hip and Vertebral Fractures in Men: the Osteoporotic Fractures in Men (MrOS)
    J Bone Miner Res, 2016-07-25;0(0):.
    Species: Human
    Sample Types: Serum
  43. Association between serum soluble tumor necrosis factor-? receptors and early childhood obesity
    Authors: S Ouyang, W Li, Z Liu, Y Li, S Li, J Wu
    Endocr J, 2016-03-31;0(0):.
    Species: Human
    Sample Types: Serum
  44. Late Antiretroviral Therapy (ART) Initiation Is Associated with Long-Term Persistence of Systemic Inflammation and Metabolic Abnormalities.
    Authors: Ghislain M, Bastard J, Meyer L, Capeau J, Fellahi S, Gerard L, May T, Simon A, Vigouroux C, Goujard C
    PLoS ONE, 2015-12-04;10(12):e0144317.
    Species: Human
    Sample Types: Serum
  45. T. vaginalis Infection Is Associated with Increased IL-8 and TNFr1 Levels but with the Absence of CD38 and HLADR Activation in the Cervix of ESN.
    Authors: Jarrett O, Brady K, Modur S, Plants J, Landay A, Ghassemi M, Golub E, Spear G, Novak R
    PLoS ONE, 2015-06-17;10(6):e0130146.
    Species: Human
    Sample Types: Cervical Fluid
  46. Plasma TNF-alpha and Soluble TNF Receptor Levels after Doxorubicin with or without Co-Administration of Mesna-A Randomized, Cross-Over Clinical Study.
    Authors: Hayslip J, Dressler E, Weiss H, Taylor T, Chambers M, Noel T, Miriyala S, Keeney J, Ren X, Sultana R, Vore M, Butterfield D, St Clair D, Moscow J
    PLoS ONE, 2015-04-24;10(4):e0124988.
    Species: Human
    Sample Types: Plasma
  47. Circulating TNF receptors 1 and 2 are associated with the severity of renal interstitial fibrosis in IgA nephropathy.
    Authors: Sonoda Y, Gohda T, Suzuki Y, Omote K, Ishizaka M, Matsuoka J, Tomino Y
    PLoS ONE, 2015-04-10;10(4):e0122212.
    Species: Human
    Sample Types: Serum
  48. A case-control study of pre-operative levels of serum neutrophil gelatinase-associated lipocalin and other potential inflammatory markers in colorectal cancer.
    Authors: Duvillard L, Ortega-Deballon P, Bourredjem A, Scherrer M, Mantion G, Delhorme J, Deguelte-Lardiere S, Petit J, Bonithon-Kopp C
    BMC Cancer, 2014-12-03;14(0):912.
    Species: Human
    Sample Types: Serum
  49. The expression of cytokines and chemokines in the blood of patients with severe weight loss from anorexia nervosa: an exploratory study.
    Authors: Pisetsky D, Trace S, Brownley K, Hamer R, Zucker N, Roux-Lombard P, Dayer J, Bulik C
    Cytokine, 2014-06-14;69(1):110-5.
    Species: Human
    Sample Types: Cell Culture Supernates
  50. Circulating levels of tumor necrosis factor-alpha receptor 2 are increased in heart failure with preserved ejection fraction relative to heart failure with reduced ejection fraction: evidence for a divergence in pathophysiology.
    Authors: Putko B, Wang Z, Lo J, Anderson T, Becher H, Dyck J, Kassiri Z, Oudit G
    PLoS ONE, 2014-06-12;9(6):e99495.
    Species: Human
    Sample Types: Plasma
  51. The effect of rosuvastatin on inflammation, matrix turnover and left ventricular remodeling in dilated cardiomyopathy: a randomized, controlled trial.
    Authors: Broch K, Askevold E, Gjertsen E, Ueland T, Yndestad A, Godang K, Stueflotten W, Andreassen J, Svendsmark R, Smith H, Aakhus S, Aukrust P, Gullestad L
    PLoS ONE, 2014-02-25;9(2):e89732.
    Species: Human
    Sample Types: Whole Blood
  52. Dynamics of immune reconstitution and activation markers in HIV+ treatment-naive patients treated with raltegravir, tenofovir disoproxil fumarate and emtricitabine.
    Authors: Funderburg N, Andrade A, Chan E, Rosenkranz S, Lu D, Clagett B, Pilch-Cooper H, Rodriguez B, Feinberg J, Daar E, Mellors J, Kuritzkes D, Jacobson J, Lederman M
    PLoS ONE, 2013-12-18;8(12):e83514.
    Species: Human
    Sample Types: Plasma
  53. IL-18-based combinatorial adjuvants promote the intranodal production of CCL19 by NK cells and dendritic cells of cancer patients.
    Authors: Wong J, Muthuswamy R, Bartlett D, Kalinski P
    Oncoimmunology, 2013-09-12;2(9):e26245.
    Species: Human
    Sample Types: Whole Cells
  54. Risk of ESRD and all cause mortality in type 2 diabetes according to circulating levels of FGF-23 and TNFR1.
    Authors: Lee J, Gohda T, Walker W, Skupien J, Smiles A, Holak R, Jeong J, McDonnell K, Krolewski A, Niewczas M
    PLoS ONE, 2013-03-20;8(3):e58007.
    Species: Human
    Sample Types: Plasma
  55. Compensated inflammation in systemic juvenile idiopathic arthritis: role of alternatively activated macrophages.
    Authors: Shimizu M, Yachie A
    Cytokine, 2012-06-08;60(1):226-32.
    Species: Human
    Sample Types: Serum
  56. Inflammatory markers and the risk of hip fracture: the Women's Health Initiative.
    Authors: Barbour KE, Boudreau R, Danielson ME, Youk AO, Wactawski-Wende J, Greep NC, LaCroix AZ, Jackson RD, Wallace RB, Bauer DC, Allison MA, Cauley JA
    J. Bone Miner. Res., 2012-05-01;27(5):1167-76.
    Species: Human
    Sample Types: Serum
  57. Inflammation marker and risk of pancreatic cancer: a nested case-control study within the EPIC cohort.
    Authors: Grote VA, Kaaks R, Nieters A, Tjonneland A, Halkjaer J, Overvad K, Skjelbo Nielsen MR, Boutron-Ruault MC, Clavel-Chapelon F, Racine A, Teucher B, Becker S, Pischon T, Boeing H, Trichopoulou A, Cassapa C, Stratigakou V, Palli D, Krogh V, Tumino R, Vineis P, Panico S, Rodriguez L, Duell EJ, Sanchez MJ, Dorronsoro M, Navarro C, Gurrea AB, Siersema PD, Peeters PH, Ye W, Sund M, Lindkvist B, Johansen D, Khaw KT, Wareham N, Allen NE, Travis RC, Fedirko V, Jenab M, Michaud DS, Chuang SC, Romaguera D, Bueno-de-Mesquita HB, Rohrmann S
    Br. J. Cancer, 2012-04-26;106(11):1866-74.
    Species: Human
    Sample Types: Serum
  58. Cytokine Response Patterns in Severe Pandemic 2009 H1N1 and Seasonal Influenza among Hospitalized Adults.
    Authors: Lee N, Wong CK, Chan PK, Chan MC, Wong RY, Lun SW, Ngai KL, Lui GC, Wong BC, Lee SK, Choi KW, Hui DS
    PLoS ONE, 2011-10-13;6(10):e26050.
    Species: Human
    Sample Types: Plasma
  59. Elevated CSF levels of TACE activity and soluble TNF receptors in subjects with mild cognitive impairment and patients with Alzheimer's disease.
    Authors: Jiang H, Hampel H, Prvulovic D, Wallin A, Blennow K, Li R, Shen Y
    Mol Neurodegener, 2011-10-06;6(0):69.
    Species: Human
    Sample Types: CSF
  60. Matrix metalloproteinase-9 and plasminogen activator inhibitor-1 are associated with right ventricular structure and function: the MESA-RV Study.
    Authors: Kawut SM, Barr RG, Johnson WC
    Biomarkers, 2010-10-05;15(8):731-8.
    Species: Human
    Sample Types: Plasma
  61. Adrenomedullin and endothelin-1 are related to inflammation in chronic heart failure.
    Authors: Gombos T, Forhecz Z, Pozsonyi Z, Wallentin S, Papassotiriou J, Kunde J, Morgenthaler NG, Janoskuti L, Prohaszka Z
    Inflamm. Res., 2009-06-01;58(6):298-305.
    Species: Human
    Sample Types: Serum
  62. Secretory lysosome targeting and induced secretion of human soluble TNF-alpha receptor in murine hematopoietic cells in vivo as a principle for immunoregulation in inflammation and malignancy.
    Authors: Johansson AC, Nandakumar KS, Persson AM, Olsson I, Hansson M
    Exp. Hematol., 2009-05-30;37(8):969-78.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  63. Selective effects of CPAP on sleep apnoea-associated manifestations.
    Authors: Vgontzas AN, Zoumakis E, Bixler EO, Lin HM, Collins B, Basta M, Pejovic S, Chrousos GP
    Eur. J. Clin. Invest., 2008-08-01;38(8):585-95.
    Species: Human
    Sample Types: Plasma
  64. Change in plasma tumor necrosis factor receptor 1 levels in the first week after myeloablative allogeneic transplantation correlates with severity and incidence of GVHD and survival.
    Authors: Choi SW, Kitko CL, Braun T, Paczesny S, Yanik G, Mineishi S, Krijanovski O, Jones D, Whitfield J, Cooke K, Hutchinson RJ, Ferrara JL, Levine JE
    Blood, 2008-05-23;112(4):1539-42.
    Species: Human
    Sample Types: Plasma
  65. Comparing Type D personality and older age as correlates of tumor necrosis factor-alpha dysregulation in chronic heart failure.
    Authors: Denollet J, Vrints CJ, Conraads VM
    Brain Behav. Immun., 2008-02-20;22(5):736-43.
    Species: Human
    Sample Types: Plasma
  66. Microparticles of human atherosclerotic plaques enhance the shedding of the tumor necrosis factor-alpha converting enzyme/ADAM17 substrates, tumor necrosis factor and tumor necrosis factor receptor-1.
    Authors: Canault M, Leroyer AS, Peiretti F, Leseche G, Tedgui A, Bonardo B, Alessi MC, Boulanger CM, Nalbone G
    Am. J. Pathol., 2007-09-14;171(5):1713-23.
    Species: Human
    Sample Types: Cell Culture Supernates
  67. Inflammatory markers and incident fracture risk in older men and women: the Health Aging and Body Composition Study.
    Authors: Cauley JA, Danielson ME, Boudreau RM, Forrest KY, Zmuda JM, Pahor M, Tylavsky FA, Cummings SR, Harris TB, Newman AB
    J. Bone Miner. Res., 2007-07-01;22(7):1088-95.
    Species: Human
    Sample Types: Serum
  68. Selective functional exhaustion of hematopoietic progenitor cells in the bone marrow of patients with postinfarction heart failure.
    Authors: Kissel CK, Lehmann R, Assmus B, Aicher A, Honold J, Fischer-Rasokat U, Heeschen C, Spyridopoulos I, Dimmeler S, Zeiher AM
    J. Am. Coll. Cardiol., 2007-06-04;49(24):2341-9.
    Species: Human
    Sample Types: Serum
  69. Muscle atrophy and hypertrophy signaling in patients with chronic obstructive pulmonary disease.
    Authors: Doucet M, Russell AP, Leger B, Debigare R, Joanisse DR, Caron MA, LeBlanc P, Maltais F
    Am. J. Respir. Crit. Care Med., 2007-05-03;176(3):261-9.
    Species: Human
    Sample Types: Plasma
  70. Serum interleukin-18 and soluble tumour necrosis factor receptor 2 are associated with disease severity in patients with paracoccidioidomycosis.
    Authors: Corvino CL, Mamoni RL, Fagundes GZ, Blotta MH
    Clin. Exp. Immunol., 2007-03-01;147(3):483-90.
    Species: Human
    Sample Types: Serum
  71. The shedding activity of ADAM17 is sequestered in lipid rafts.
    Authors: Tellier E, Rebsomen L
    Exp. Cell Res., 2006-09-05;312(20):3969-80.
    Species: Primate - Cholrocebus pygerythrus (Vervet Monkey)
    Sample Types: Cell Lysates
  72. Proinflammatory cytokines, insulin resistance, and insulin secretion in chronic hepatitis C patients: A case-control study.
    Authors: Lecube A, Hernandez C, Genesca J, Simo R
    Diabetes Care, 2006-05-01;29(5):1096-101.
    Species: Human
    Sample Types: Serum
  73. Neutrophils from patients with TNFRSF1A mutations display resistance to tumor necrosis factor-induced apoptosis: pathogenetic and clinical implications.
    Authors: D&amp;apos;Osualdo A
    Arthritis Rheum., 2006-03-01;54(3):998-1008.
    Species: Human
    Sample Types: Cell Culture Supernates
  74. Experimental heatstroke in baboon: analysis of the systemic inflammatory response.
    Authors: Bouchama A, Ollivier V, Roberts G, Al Mohanna F, de Prost D, Eldali A, Saussereau E, El-Sayed R, Chollet-Martin S
    Shock, 2005-10-01;24(4):332-5.
    Species: Primate - Papio hamadryas (Hamadyras Baboon)
    Sample Types: Plasma
  75. Effects of the active metabolite of leflunomide, A77 1726, on cytokine release and the MAPK signalling pathway in human rheumatoid arthritis synoviocytes.
    Authors: Vergne-Salle</LastName><F P</Initial, Vergne-Salle P, Leger DY, Bertin P, Treves R, Beneytout JL, Liagre B
    Cytokine, 2005-09-07;31(5):335-48.
    Species: Human
    Sample Types: Cell Culture Supernates
  76. Regulation of peritoneal and systemic neutrophil-derived tumor necrosis factor-alpha release in patients with severe peritonitis: role of tumor necrosis factor-alpha converting enzyme cleavage.
    Authors: Kermarrec N, Selloum S, Plantefeve G, Chosidow D, Paoletti X, Lopez A, Mantz J, Desmonts JM, Gougerot-Pocidalo MA, Chollet-Martin S
    Crit. Care Med., 2005-06-01;33(6):1359-64.
    Species: Human
    Sample Types: Cell Culture Supernates
  77. Levels of soluble cytokine factors in temporomandibular joint effusions seen on magnetic resonance images.
    Authors: Kaneyama K, Segami N, Sun W, Sato J, Fujimura K
    Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 2005-04-01;99(4):411-8.
    Species: Human
    Sample Types: Synovial Fluid
  78. Growth hormone-induced reduction of soluble apoptosis mediators is associated with reverse cardiac remodelling and improvement of exercise capacity in patients with idiopathic dilated cardiomyopathy.
    Authors: Parissis JT, Adamopoulos S, Karatzas D, Paraskevaidis J, Livanis E, Kremastinos D
    Eur J Cardiovasc Prev Rehabil, 2005-04-01;12(2):164-8.
    Species: Human
    Sample Types: Plasma
  79. Analysis of tumor necrosis factor-alpha, interleukin-6, interleukin-1beta, soluble tumor necrosis factor receptors I and II, interleukin-6 soluble receptor, interleukin-1 soluble receptor type II, interleukin-1 receptor antagonist, and protein in the synovial fluid of patients with temporomandibular joint disorders.
    Authors: Kaneyama K, Segami N, Sun W, Sato J, Fujimura K
    Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 2005-03-01;99(3):276-84.
    Species: Human
    Sample Types: Synovial Fluid
  80. Gene transfer of tumor necrosis factor inhibitor improves the function of lung allografts.
    Authors: Tagawa T, Kozower BD, Kanaan SA, Daddi N, Muraoka M, Oka T, Ritter JH, Patterson GA
    J. Thorac. Cardiovasc. Surg., 2004-06-01;127(6):1558-63.
    Species: Human
    Sample Types: Tissue Homogenates
  81. Pro- and anti-inflammatory responses in respiratory syncytial virus bronchiolitis.
    Authors: McNamara PS, Flanagan BF, Selby AM, Hart CA, Smyth RL
    Eur. Respir. J., 2004-01-01;23(1):106-12.
    Species: Human
    Sample Types: BALF
  82. Arenavirus-mediated liver pathology: acute lymphocytic choriomeningitis virus infection of rhesus macaques is characterized by high-level interleukin-6 expression and hepatocyte proliferation.
    Authors: Lukashevich IS, Tikhonov I, Rodas JD, Zapata JC, Yang Y, Djavani M, Salvato MS
    J. Virol., 2003-02-01;77(3):1727-37.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Serum
  83. Age-related autoantibody production in a nonhuman primate model.
    Authors: Attanasio R, Brasky KM, Robbins SH, Jayashankar L, Nash RJ, Butler TM
    Clin. Exp. Immunol., 2001-03-01;123(3):361-5.
    Species: Primate - Papio cynocephalus (Yellow Baboon)
    Sample Types: Serum
  84. An analysis of 14 molecular markers for monitoring osteoarthritis: segregation of the markers into clusters and distinguishing osteoarthritis at baseline.
    Authors: Otterness IG, Swindell AC, Zimmerer RO, Poole AR, Ionescu M, Weiner E
    Osteoarthr. Cartil., 2000-05-01;8(3):180-5.
    Species: Human
    Sample Types: Serum
  85. Proinflammatory cytokines increase in sepsis after anti-adhesion molecule therapy.
    Authors: Welty-Wolf KE, 2016, Carraway MS, Ghio A, Kantrow SP, Huang YC, Piantadosi CA
    1-23, 2000-05-01;13(5):404-9.
    Species: Primate - Papio cynocephalus (Yellow Baboon)
    Sample Types: Serum
  86. Tumour necrosis factor alpha and its soluble receptors in juvenile chronic arthritis.
    Authors: Rooney M, Varsani H, Martin K, Lombard PR, Dayer JM, Woo P
    Rheumatology (Oxford), 2000-04-01;39(4):432-8.
    Species: Human
    Sample Types: Serum

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Human TNF RI/TNFRSF1A Quantikine ELISA Kit
By Anonymous on 07/06/2023
Sample Tested: Plasma
Human TNF RI/TNFRSF1A Quantikine ELISA Kit DRT100
Human TNF RI/TNFRSF1A Quantikine ELISA Kit
By Anonymous on 09/21/2022
Sample Tested: Serum
Human TNF RI/TNFRSF1A Quantikine ELISA Kit DRT100
Human TNF RI/TNFRSF1A Quantikine ELISA Kit
By James Williams on 10/13/2021
Sample Tested: EDTA Plasma
Human TNF RI/TNFRSF1A Quantikine ELISA Kit DRT100
Human TNF RI/TNFRSF1A Quantikine ELISA Kit
By Anonymous on 12/06/2019
Sample Tested: Jurkat human acute T cell leukemia cell line