Human TNF RII/TNFRSF1B Fluorescein-conjugated Antibody

Name: Human TNF RII/TNFRSF1B Fluorescein-conjugated Antibody
Brand: R&D Systems
SKU: FAB226F
Price: 291 USD

Catalog #: FAB226F
6 Citations Datasheet / COA / SDS

Newer Version Available: FAB226G

NEW

Discontinued Product

FAB226F has been discontinued and is replaced by FAB226R. FAB2262N. FAB226S. FAB226T. FAB226G.

Detection of TNF RII/TNFRSF1B in Human Granulocytes by Flow Cytometry.

1 Image

All TNF RII/TNFRSF1B Antibodies

Product Details

Citations (6)

FAQs

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TNF RII/TNFRSF1B Fluorescein-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human TNF RII in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with recombinant human (rh) 4‑1BB, rhBAFF R, rhCD27, rhCD30, rhCD40, rhDR3, rhDR6, rhEDAR, rhFas, rhGITR, rhHVEM, rhLymophotactin beta R, rhNGF R, rhOPG, recombinant mouse (rm) OX40, rhRANK, rhTAJ, rhTNF RI, or rmTNF RII.

Source

Monoclonal Mouse IgG_2A Clone # 22235

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human TNF RII
Extracellular domain

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

View Larger

Detection of TNF RII/TNFRSF1B in Human Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Mouse Anti-Human TNF RII/TNFRSF1B Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB226F, filled histogram) or isotype control antibody (Catalog # IC003F, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: TNF RII/TNFRSF1B

TNF RII is a type I transmembrane glycoprotein primarily expressed on hematopoietic cells and is designated TNFRSF1B. TNF RII is a high-affinity receptor for TNF‑ alpha and TNF-beta. A soluble form of the receptor generated by proteolytic cleavage functions as a TNF antagonist.

Long Name

Tumor Necrosis Factor Receptor II

Entrez Gene IDs

7133 (Human); 21938 (Mouse); 156767 (Rat); 102144224 (Cynomolgus Monkey)

Alternate Names

CD120b antigen; CD120b; Etanercept; p75 TNF receptor; p75TBPII; p75TNFR; soluble TNFR1B variant 1; TNF RII; TNFBRp80 TNF-alpha receptor; TNF-R2; TNFR2TNFR1B; TNF-R75; TNFR80; TNFRII; TNF-RII; TNF-R-II; TNFR-II; TNFRSF1B; tumor necrosis factor beta receptor; tumor necrosis factor binding protein 2; Tumor necrosis factor receptor 2; tumor necrosis factor receptor superfamily member 1B; tumor necrosis factor receptor superfamily, member 1B; Tumor necrosis factor receptor type II

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human TNF RII/TNFRSF1B Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
Filter your results:

Filter by:

The lytic polysaccharide monooxygenase CbpD promotes Pseudomonas aeruginosa virulence in systemic infection
Authors: F Askarian, S Uchiyama, H Masson, HV Sørensen, O Golten, AC Bunæs, S Mekasha, ÅK Røhr, E Kommedal, JA Ludviksen, MØ Arntzen, B Schmidt, RH Zurich, NM van Sorge, VGH Eijsink, U Krengel, TE Mollnes, NE Lewis, V Nizet, G Vaaje-Kols
Nature Communications, 2021-02-23;12(1):1230.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
TNFR2 and IL-12 coactivation enables slanDCs to support NK-cell function via membrane-bound TNF-alpha.
Authors: Tufa D, Chatterjee D, Low H, Schmidt R, Jacobs R
Eur J Immunol, 2014-10-29;44(12):3717-28.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Early and nonreversible decrease of CD161++ /MAIT cells in HIV infection.
Authors: Cosgrove C, Ussher J, Rauch A, Gartner K, Kurioka A, Huhn M, Adelmann K, Kang Y, Fergusson J, Simmonds P, Goulder P, Hansen T, Fox J, Gunthard H, Khanna N, Powrie F, Steel A, Gazzard B, Phillips R, Frater J, Uhlig H, Klenerman P
Blood, 2012-12-18;121(6):951-61.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Staphylococcus aureus Staphopain A inhibits CXCR2-dependent neutrophil activation and chemotaxis.
EMBO J., 2012-07-31;31(17):3607-19.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Staphylococcal superantigen-like 5 binds PSGL-1 and inhibits P-selectin-mediated neutrophil rolling.
Authors: Bestebroer J, Poppelier MJ, Ulfman LH, Lenting PJ, Denis CV, van Kessel KP, van Strijp JA, de Haas CJ
Blood, 2007-04-01;109(7):2936-43.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Simultaneous induction of apoptotic and survival signaling pathways in macrophage-like THP-1 cells by Shiga toxin 1.
Authors: Lee SY, Cherla RP, Tesh VL
Infect. Immun., 2006-12-28;75(3):1291-302.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry