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Human Total HSP27 DuoSet IC ELISA

Catalog #: DYC1580-2
4 Citations 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
DYC1580E
DYC1580-5
DYC1580-2
Ancillary Products Available
DY999B: Substrate Reagent Pack
DY999: Substrate Reagent Pack
Standard Curve
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Product Details
Citations (4)
FAQs
Supplemental Products
Reviews (2)
Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Human Total HSP27 DuoSet IC ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4 hours 40 mins (after plate preparation)
Sample Volume Required
Cell lysates (100 µL)
Assay Range
31.2 - 2,000 pg/mL
Sufficient Materials
Kits available for two, five, or fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure total HSP27 in cell lysates. An immobilized capture antibody specific for HSP27 binds both phosphorylated and unphosphorylated HSP27. After washing away unbound material, a biotinylated detection antibody is used to detect both phosphorylated and unphosphorylated protein, utilizing a standard Streptavidin-HRP format.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Available in 2, 5, and 15- (96-well) plate pack sizes
  • Economical alternative to Western blot

Kit Content

  • Capture Antibody
  • Conjugated Detection Antibody
  • Calibrated Immunoassay Standard or Control
  • Streptavidin-HRP

Other Reagents Required


PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Lysis Buffer*

IC Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: From Costar EIA Plate (Costar Catalog # 2592) or R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product

Scientific Data

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Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: HSP27

The heat shock proteins are a highly conserved family of stress response proteins. HSPs function primarily as molecular chaperones, facilitating the folding of other cellular proteins, preventing protein aggregation, or targeting improperly folded proteins to specific degradative pathways. Some HSPs are expressed at low levels under normal physiological conditions but show dramatically increased expression in response to cellular stress, others are constitutively expressed. Specific HSPs play a role in regulating apoptosis by interacting directly with key components of the apoptotic pathway.

Long Name:
Heat Shock Protein 27
Entrez Gene IDs:
3315 (Human); 15510 (Mouse); 24471 (Rat)
Alternate Names:
28 kDa heat shock protein; DKFZp586P1322; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; heat shock 27kD protein 1; heat shock 27kDa protein 1; heat shock protein beta-1; HMN2B; HS.76067; HSP25; HSP27; HSP27HSP 27; HSP28CMT2F; HSPB1; SRP27; Stress-responsive protein 27

Citations for Human Total HSP27 DuoSet IC ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Systemic release of heat-shock protein 27 and 70 following severe trauma
    Authors: T Haider, E Simader, O Glück, HJ Ankersmit, T Heinz, S Hajdu, LL Negrin
    Sci Rep, 2019-07-03;9(1):9595.
    Species: Human
    Sample Types: Serum
  2. Heat Shock Protein 27 Plays a Pivotal Role in Myofibroblast Differentiation and in the Development of Bleomycin-Induced Pulmonary Fibrosis.
    Authors: Park A, Kanai K, Itoh T, Sato T, Tsukui T, Inagaki Y, Selman M, Matsushima K, Yoshie O
    PLoS ONE, 2016-02-09;11(2):e0148998.
    Species: Human
    Sample Types: BALF
  3. Stromal expression of heat-shock protein 27 is associated with worse clinical outcome in patients with colorectal cancer lung metastases.
    Authors: Schweiger T, Nikolowsky C, Starlinger P, Traxler D, Zimmermann M, Birner P, Hegedus B, Dome B, Bergmann M, Mildner M, Klepetko W, Hoetzenecker K, Ankersmit H
    PLoS ONE, 2015-03-20;10(3):e0120724.
    Species: Human
    Sample Types: Serum
  4. Proteomic alterations in heat shock protein 27 and identification of phosphoproteins in ascending aortic aneurysm associated with bicuspid and tricuspid aortic valve.
    Authors: Matt P, Fu Z, Carrel T, Huso D, Dirnhofer S, Lefkovits I, Zerkowski H, Van Eyk J
    J. Mol. Cell. Cardiol., 2007-08-28;43(6):792-801.
    Species: Human
    Sample Types: Tissue Homogenates

FAQs

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Reviews for Human Total HSP27 DuoSet IC ELISA

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Human Total HSP27 DuoSet IC ELISA
By Anonymous on 12/24/2018
Sample Tested: Human Renal Epithelial Cell Lysis
Human Total HSP27 DuoSet IC ELISA DYC1580-2
Human Total HSP27 DuoSet IC ELISA
By Neda Ghousifam on 08/17/2018
Sample Tested: Human Renal Epithelial Cell Lysates,Human Renal Epithelial Cells

I have used this kit to detect HSP27 expression in Human Renal Epithelial Cell lysates. Attached is the standard curve that I got from one plate.

Human Total HSP27 DuoSet IC ELISA DYC1580-2