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Human Total IL-18/IL-1F4 Quantikine ELISA Kit

Catalog #: DL180
25 Citations 4 Reviews Datasheet / COA / SDS
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Human Total IL-18 ELISA Cell Culture Supernate/Urine Standard Curve
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Summary Sample Values Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human Total IL-18/IL-1F4 Quantikine ELISA Kit Summary

Assay Length
4.0 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (17 uL), EDTA Plasma (17 uL), Heparin Plasma (17 uL), Saliva (25 uL), Urine (50 uL)
Sensitivity
5.15 pg/mL
Assay Range
15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine)
Specificity
Natural and recombinant human Total IL-18.
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules.Cross-species reactivity observed with 1 or more species tested.
Interference
No significant interference observed with available related molecules.

Sample Values

Serum/Plasma/Saliva/Urine - Samples from apparently healthy volunteers were evaluated for the presence of human Total IL-18 in this assay. No medical histories were available for the donors used in this study.

Sample TypeMean (pg/mL)Range (pg/mL)Standard Devitation (pg/mL)
Serum (n=30)18384.8-35869.7
EDTA plasma (n=30)18381.5-34468.1
Heparin plasma18287.5-33365.8

Sample TypeMean of Detectable (pg/mL)% DetectableRange (pg/mL)
Saliva (n=10)57.170ND-69.8
Urine (n=10)44.030ND-74.0
ND=Non-detectable

Cell Culture Supernates - THP-1 human acute monocytic leukemia cells were cultured in RPMI supplemented with 10% fetal bovine serum, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. Cells were cultured unstimulated or stimulated with 1 μg/mL LPS for 5 hours followed by 20 μM Nigericin for 1 hour. Aliquots of the cell culture supernates were removed and assayed for levels of human Total IL-18.

Condition(pg/mL)
Unstimulated19.6
Stimulated562





Product Summary

The Quantikine® Human Total IL-18/IL-1F4 Immunoassay is a 4.0 hour solid-phase ELISA designed to measure human IL-18 in cell culture supernates, serum, plasma, saliva, and urine. It contains E. coli-expressed recombinant human IL-18 and has been shown to accurately quantitate the recombinant factor free and in complex with IL-18 BPa. Results obtained using natural human IL-18 showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-18.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Cell Culture Supernates, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 175 301 636 197 381 739
Standard Deviation 5.49 8.06 24.6 27.4 46.7 81.1
CV% 3.1 2.7 3.9 13.9 12.3 11

Serum, EDTA Plasma, Heparin Plasma, Saliva

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 126 251 495 126 248 494
Standard Deviation 3.85 6.33 14.6 10.9 19.7 41.6
CV% 3.1 2.5 2.9 8.7 7.9 8.4

Recovery

The recovery of human Total IL-18 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 96 90-102
EDTA Plasma (n=4) 104 93-113
Heparin Plasma (n=4) 102 88-110
Saliva (n=4) 108 94-120
Serum (n=4) 100 96-105
Urine (n=4) 91 84-100

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human Total IL-18 were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Human Total IL-18 ELISA Linearity

Scientific Data

N/A Human Total IL-18 ELISA Cell Culture Supernate/Urine Standard Curve View Larger

Human Total IL-18 ELISA Cell Culture Supernate/Urine Standard Curve

N/A Human Total IL-18 ELISA Serum/Plasma/Saliva Standard Curve View Larger

Human Total IL-18 ELISA Serum/Plasma/Saliva Standard Curve

Product Datasheets

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Product Datasheet
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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-18/IL-1F4

Interleukin-18 (IL-18) is a proinflammatory cytokine in the IL-1 family that exerts distinct immune effects depending on the local cytokine environment. It is expressed as a 24 kDa precursor by endothelial and epithelial cells, keratinocytes, gamma δT cells, and phagocytes. The precursor is activated intracellularly by Caspase-1 mediated proteolysis to release the 17 kDa mature cytokine. The precursor can also be released by necrotic cells for extracellular cleavage by multiple proteases. IL-18 activation is induced by infection or tissue damage and contributes to disease pathology in chronic inflammation (1-3). IL-18 binds to the widely expressed IL-18 R alpha which recruits IL-18 R beta to form the signaling receptor complex (4, 5). Its bioactivity is negatively regulated by interactions with IL-18 binding proteins and virally encoded IL-18BP homologs (6). In the presence of IL-12 or IL-15, IL-18 enhances anti-viral Th1 immune responses by inducing IFN-gamma production and the cytolytic activity of CD8+ T cells and NK cells (7, 8). In the absence of IL-12 or IL-15, however, IL-18 promotes production of the Th2 cytokines IL-4 and IL-13 by CD4+ T cells and basophils (9, 10). In the presence of IL-1 beta or IL-23, IL-18 induces the antigen independent production of IL-17 by gamma δ T cells and CD4+ T cells (11). IL-18 also promotes myeloid dendritic cell maturation and triggers neutrophil respiratory burst (12, 13). In cancer, IL-18 exhibits diverse activities including enhancing anti-tumor immunity, inhibiting or promoting angiogenesis, and promoting tumor cell metastasis (14). Mature human IL-18 shares approximately 63% amino acid sequence identity with mouse and rat IL-18 (15). Alternative splicing in human ovarian cancer generates an isoform that is resistant to Caspase-1 activation (16). A cell surface form can be expressed on M-CSF induced macrophages and released in response to bacterial endotoxin (17).

Long Name:
Interleukin 18
Entrez Gene IDs:
3606 (Human); 16173 (Mouse); 29197 (Rat); 397057 (Porcine); 574151 (Primate)
Alternate Names:
Iboctadekin; IFN-gamma-inducing factor; IGIF; IGIFIL-1 gamma; IL18; IL-18; IL-18MGC12320; IL-1F4; IL1F4iboctadekin; IL-1g; Interferon gamma-inducing factor; interleukin 18 (interferon-gamma-inducing factor); Interleukin-1 gamma; interleukin-18
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human Total IL-18/IL-1F4 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

25 Citations: Showing 1 - 10
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  1. Evaluating Single-Nucleotide Polymorphisms in Inflammasome Proteins and Serum Levels of IL-18 and IL-1? in Kidney Interstitial Damage in Anti-Neutrophilic Cytoplasmic Antibody-Associated Vasculitis
    Authors: Martinez Valenzuela, L;Vidal-Alabró, A;Rubio, B;Antón-Pàmpols, P;Gómez-Preciado, F;Fulladosa, X;Cruzado, JM;Torras, J;Lloberas, N;Draibe, J;
    International journal of molecular sciences
    Species: Human
    Sample Types: Urine
  2. The Impact of Metabolic Memory on Immune Profile in Young Patients with Uncomplicated Type 1 Diabetes
    Authors: Neubauer-Geryk, J;Wielicka, M;My?liwiec, M;Zorena, K;Bieniaszewski, L;
    International journal of molecular sciences
    Species: Human
    Sample Types: Serum
  3. VEXAS syndrome is characterized by inflammasome activation and monocyte dysregulation
    Authors: Kosmider, O;Possémé, C;Templé, M;Corneau, A;Carbone, F;Duroyon, E;Breillat, P;Chirayath, TW;Oules, B;Sohier, P;Luka, M;Gobeaux, C;Lazaro, E;Outh, R;Le Guenno, G;Lifermann, F;Berleur, M;Le Mene, M;Friedrich, C;Lenormand, C;Weitten, T;Guillotin, V;Burroni, B;Boussier, J;Willems, L;Aractingi, S;Dionet, L;Tharaux, PL;Vergier, B;Raynaud, P;Ea, HK;Ménager, M;Duffy, D;Terrier, B;
    Nature communications
    Species: Human
    Sample Types: Plasma
  4. The tetrapeptide sequence of IL-18 and IL-1? regulates their recruitment and activation by inflammatory caspases
    Authors: Exconde, PM;Hernandez-Chavez, C;Bourne, CM;Richards, RM;Bray, MB;Lopez, JL;Srivastava, T;Egan, MS;Zhang, J;Yoo, W;Shin, S;Discher, BM;Taabazuing, CY;
    Cell reports
    Species: Human
    Sample Types: Cell Culture Supernates
  5. Increased Levels of Caspase-1 and IL-1? Among Adults With Persistent Immune Activation After 12 Years of Suppressive Antiretroviral Therapy in the Infectious Diseases Institute HIV Treatment Cohort
    Authors: Nabatanzi, R;Ssekamatte, P;Castelnuovo, B;Kambugu, A;Nakanjako, D;
    Open forum infectious diseases
    Species: Human
    Sample Types: Plasma
  6. KIM-1, IL-18, and NGAL, in the Machine Learning Prediction of Kidney Injury among Children Undergoing Hematopoietic Stem Cell Transplantation-A Pilot Study
    Authors: Musia?, K;Stojanowski, J;Mi?kiewicz-Bujna, J;Ka?wak, K;Ussowicz, M;
    International journal of molecular sciences
    Species: Human
    Sample Types: Serum
  7. Coronary Artery Plaque Composition and Severity Relate to the Inflammasome in People With Treated Human Immunodeficiency Virus
    Authors: SR Schnittman, DW Kitch, TH Swartz, TH Burdo, KV Fitch, S McCallum, JM Flynn, ES Fulda, MR Diggs, JT Stapleton, JL Casado, J Taron, JS Currier, MV Zanni, C Malvestutt, CJ Fichtenbau, JA Aberg, HJ Ribaudo, MT Lu, PS Douglas, SK Grinspoon
    Open forum infectious diseases, 2023-03-01;10(3):ofad106.
    Species: Human
    Sample Types: Plasma
  8. Vascular Function, Systemic Inflammation, and Coagulation Activation 18 Months after COVID-19 Infection: An Observational Cohort Study
    Authors: LH Willems, LMC Jacobs, LA Groh, H Ten Cate, HMH Spronk, B Wilson-Sto, G Hannink, SMJ van Kuijk, C Ghossein-D, M Nagy, DHJ Thijssen, AS van Peters, MC Warlé
    Journal of Clinical Medicine, 2023-02-10;12(4):.
    Species: Human
    Sample Types: Plasma
  9. N-acetylcysteine Reduces Inflammasome Activation Induced by SARS-CoV-2 Proteins In Vitro
    Authors: J Milara, F Martínez-E, P Montero, I Roger, MA Bayarri, P Ribera, MN Oishi-Kona, JR Alba-Garcí, E Zapater, J Cortijo
    International Journal of Molecular Sciences, 2022-11-22;23(23):.
    Species: Human
    Sample Types: Cell Culture Supernates
  10. Serum Levels of Selected IL-1 Family Cytokines in Patients with Morphea
    Authors: P Szczepanik, M Michalska-, M Kowal, D Krasowska
    Journal of Clinical Medicine, 2022-10-28;11(21):.
    Species: Human
    Sample Types: Serum
  11. Resveratrol Mitigates Oxygen and Glucose Deprivation-Induced Inflammation, NLRP3 Inflammasome, and Oxidative Stress in 3D Neuronal Culture
    Authors: MC Chiang, CJB Nicol, SS Lo, SW Hung, CJ Wang, CH Lin
    International Journal of Molecular Sciences, 2022-10-02;23(19):.
    Species: Human
    Sample Types: Cell Culture Supernates
  12. Modulation of Neuroendocrine and Immunological Biomarkers Following Rehabilitation in Sarcopenic Patients
    Authors: F Piancone, F La Rosa, I Marventano, A Hernis, R Miglioli, F Trecate, M Saresella, M Clerici
    Cells, 2022-08-10;11(16):.
    Species: Human
    Sample Types: Plasma
  13. Modulation of MAPK- and PI3/AKT-Dependent Autophagy Signaling by Stavudine (D4T) in PBMC of Alzheimer's Disease Patients
    Authors: F La Rosa, CP Zoia, C Bazzini, A Bolognini, M Saresella, E Conti, C Ferrarese, F Piancone, I Marventano, D Galimberti, C Fenoglio, E Scarpini, M Clerici
    Cells, 2022-07-12;11(14):.
    Species: Human
    Sample Types: Cell Culture Supernates
  14. Cytokine response over the course of COVID-19 infection in pregnant women
    Authors: DB Rosen, EA Murphy, RS Gejman, A Capili, RL Friedlande, S Rand, KA Cagino, SM Glynn, KC Matthews, JM Kubiak, J Yee, M Prabhu, LE Riley, YJ Yang
    Cytokine, 2022-04-25;154(0):155894.
    Species: Human
    Sample Types: Serum
  15. The Effects of Beverage Intake after Exhaustive Exercise on Organ Damage, Inflammation and Oxidative Stress in Healthy Males.
    Authors: Tominaga T, Ikemura T, Yada K, Kanda K, Sugama K, Ma S, Choi W, Araya M, Huang J, Nakamura N, Suzuki K
    Antioxidants (Basel), 2021-05-28;10(6):.
    Species: Human
    Sample Types: Urine
  16. Deregulation of IL-37 and its miRNAs modulators in sarcopenic patients after rehabilitation
    Authors: F La Rosa, S Agostini, M Saresella, AS Costa, F Piancone, R Miglioli, F Trecate, M Clerici
    Journal of Translational Medicine, 2021-04-26;19(1):172.
    Species: Human
    Sample Types: Plasma
  17. Progesterone and vitamin D downregulate the activation of the NLRP1/NLRP3 inflammasomes and TLR4-MyD88-NF-&kappaB pathway in monocytes from pregnant women with preeclampsia
    Authors: ML Matias, M Romao-Veig, VR Ribeiro, PR Nunes, VJ Gomes, AC Devides, VT Borges, GG Romagnoli, JC Peracoli, MT Peracoli
    Journal of reproductive immunology, 2021-02-05;144(0):103286.
    Species: Human
    Sample Types: Cell Culture Supernates
  18. Reduced concentrations of the B cell cytokine Interleukin 38 are associated with cardiovascular disease risk in overweight subjects
    Authors: DM de Graaf, M Jaeger, ICL van den Mu, RT Horst, K Schraa, J Zwaag, M Kox, M Fujita, T Yamauchi, L Mercurio, S Madonna, JHW Rutten, J de Graaf, NP Riksen, FL van de Vee, MG Netea, LAB Joosten, CA Dinarello
    Eur J Immunol, 2020-11-19;0(0):.
    Species: Human
    Sample Types: Plasma
  19. Short-chain fatty acids bind to apoptosis-associated speck-like protein to activate inflammasome complex to prevent Salmonella infection
    Authors: H Tsugawa, Y Kabe, A Kanai, Y Sugiura, S Hida, S Taniguchi, T Takahashi, H Matsui, Z Yasukawa, H Itou, K Takubo, H Suzuki, K Honda, H Handa, M Suematsu
    PLoS Biol, 2020-09-29;18(9):e3000813.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  20. SGLT2 inhibition modulates NLRP3 inflammasome activity via ketones and insulin in diabetes with cardiovascular disease
    Authors: SR Kim, SG Lee, SH Kim, JH Kim, E Choi, W Cho, JH Rim, I Hwang, CJ Lee, M Lee, CM Oh, JY Jeon, HY Gee, JH Kim, BW Lee, ES Kang, BS Cha, MS Lee, JW Yu, JW Cho, JS Kim, YH Lee
    Nat Commun, 2020-05-01;11(1):2127.
    Species: Human
    Sample Types: Serum
  21. Possible link of genetic variants to autoimmunity in GAD-antibody-associated neurological disorders
    Authors: FS Thaler, B Bangol, M Biljecki, J Havla, AM Schumacher, T Kümpfel
    J. Neurol. Sci., 2020-04-29;413(0):116860.
    Species: Human
    Sample Types: Serum
  22. A central role for P2X7 receptors in human microglia
    Authors: L Janks, CVR Sharma, TM Egan
    J Neuroinflammation, 2018-11-21;15(1):325.
    Species: Human
    Sample Types: Cell Culture Supernates
  23. Decreased interleukin-11 levels in the semen of infertile males
    Authors: L Qian
    Cytokine, 2018-03-22;108(0):57-59.
    Species: Human
    Sample Types: Semen
  24. Inflammation and Trajectory of Renal Function in Community-Dwelling Older Adults
    Authors: S Salimi, MD Shardell, SL Seliger, S Bandinelli, JM Guralnik, L Ferrucci
    J Am Geriatr Soc, 2018-01-23;0(0):.
    Species: Human
    Sample Types: Serum
  25. Aberrant monocyte responses predict and characterize dengue virus infection in individuals with severe disease
    Authors: YK Yong, HY Tan, SH Jen, EM Shankar, SK Natkunam, J Sathar, R Manikam, SD Sekaran
    J Transl Med, 2017-05-31;15(1):121.
    Species: Human
    Sample Types: Plasma

FAQs

  1. Does the Human Total IL-18/IL-1F4 Quantikine ELISA Kit recognize both free and bound IL-18?

    • The Human Total IL-18/IL-1F4 Quantikine ELISA Kit detects free and complexed Human IL-18 in validated sample types.

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Reviews for Human Total IL-18/IL-1F4 Quantikine ELISA Kit

Average Rating: 5 (Based on 4 Reviews)

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Human Total IL-18/IL-1F4 Quantikine ELISA Kit
By Anonymous on 02/25/2023
Sample Tested: Serum and Plasma
Human Total IL-18/IL-1F4 Quantikine ELISA Kit
By Anonymous on 01/27/2021
Sample Tested: Serum

Curve developed beautifully, kit is easy to use.

Human Total IL-18/IL-1F4 Quantikine ELISA Kit DL180
Human Total IL-18/IL-1F4 Quantikine ELISA Kit
By Anonymous on 01/27/2021
Sample Tested: Serum

Picture of the plate before adding the stop solution.
Standard curve in duplicate in columns 1 and 2.
Assay ran in duplicate.
Clear, precise and easy to follow directions.

Human Total IL-18/IL-1F4 Quantikine ELISA Kit DL180
Human Total IL-18/IL-1F4 Quantikine ELISA Kit
By Anonymous on 01/25/2020
Sample Tested: Serum and Plasma