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Human Total PON1 DuoSet IC ELISA

Catalog #: DYC5816-2
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
DYC5816-5
DYC5816-2
Ancillary Products Available
DY999B: Substrate Reagent Pack
DY999: Substrate Reagent Pack
Standard Curve
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Product Details
Citations (4)
FAQs
Supplemental Products
Reviews
Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Human Total PON1 DuoSet IC ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4 hours 40 mins (after plate preparation)
Sample Volume Required
Cell lysates (100 µL)
Assay Range
156.0 - 10,000 pg/mL
Sufficient Materials
Kits available for two, five, or fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure total PON1 in cell lysates. An immobilized capture antibody specific for PON1 binds both phosphorylated and unphosphorylated PON1. After washing away unbound material, a biotinylated detection antibody is used to detect both phosphorylated and unphosphorylated protein, utilizing a standard Streptavidin-HRP format.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Available in 2, 5, and 15- (96-well) plate pack sizes
  • Economical alternative to Western blot

Kit Content

  • Capture Antibody
  • Conjugated Detection Antibody
  • Calibrated Immunoassay Standard or Control
  • Streptavidin-HRP

Other Reagents Required


PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Lysis Buffer*

IC Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: From Costar EIA Plate (Costar Catalog # 2592) or R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product

Scientific Data

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: PON1

PON (paraoxonase/lactonase) family members PON1, PON2 and PON3 are calcium-dependent antioxidant enzymes. PON1 is secreted into the bloodstream associated with high-density lipoproteins (HDL). Serum PON1 concentrations vary widely among normal individuals, in part due to differential expression of some polymorphisms. The antioxidant activity of PON1 and other PON members may slow the initiation and progression of atherosclerosis.

Long Name:
Paraoxonase 1
Entrez Gene IDs:
5444 (Human); 18979 (Mouse); 84024 (Rat)
Alternate Names:
A-esterase 1; Aromatic esterase 1; arylesterase B-type; EC 3.1.1.2; EC 3.1.1.81; EC 3.1.8.1; ESA; ESAesterase A; K-45; MVCD5; paraoxonase 1; PON 1; PON1; PONparaoxonase B-type; serum aryldiakylphosphatase; Serum aryldialkylphosphatase 1; serum paraoxonase/arylesterase 1

Citations for Human Total PON1 DuoSet IC ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Increased miR-142 Levels in Plasma and Atherosclerotic Plaques from Peripheral Artery Disease Patients with Post-Surgery Cardiovascular Events
    Authors: T Barbalata, OE Moraru, CS Stancu, Y Devaux, M Simionescu, AV Sima, LS Niculescu
    International Journal of Molecular Sciences, 2020-12-16;21(24):.
    Species: Human
    Sample Types: Plasma
  2. Lipid metabolism impairment in patients with sepsis secondary to hospital acquired pneumonia, a proteomic analysis
    Authors: NK Sharma, BL Ferreira, AK Tashima, MKC Brunialti, RJS Torquato, A Bafi, M Assuncao, LCP Azevedo, R Salomao
    Clin Proteomics, 2019-07-16;16(0):29.
    Species: Human
    Sample Types: Plasma
  3. Circulating Lactonase Activity but Not Protein Level of PON-1 Predicts Adverse Outcomes in Subjects with Chronic Kidney Disease
    Authors: CJ Mohammed, Y Xie, PS Brewster, S Ghosh, P Dube, T Sarsour, AL Kleinhenz, EL Crawford, D Malhotra, RW James, PA Kalra, ST Haller, DJ Kennedy
    J Clin Med, 2019-07-15;8(7):.
    Species: Human
    Sample Types: Plasma
  4. Dysfunctional high-density lipoproteins have distinct composition, diminished anti-inflammatory potential and discriminate acute coronary syndrome from stable coronary artery disease patients
    Authors: MG Carnuta, CS Stancu, L Toma, GM Sanda, LS Niculescu, M Deleanu, AC Popescu, MR Popescu, A Vlad, DR Dimulescu, M Simionescu, AV Sima
    Sci Rep, 2017-08-04;7(1):7295.
    Species: Human
    Sample Types: Serum

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