Human Total Survivin DuoSet IC ELISA

Catalog #: DYC647-2
12 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
DYC647E
DYC647-5
DYC647-2

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Citations (12)

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Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Human Total Survivin DuoSet IC ELISA Summary

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4 hours 40 mins (after plate preparation)

Sample Volume Required

Cell lysates (100 µL)

Assay Range

62.5 - 4,000 pg/mL

Sufficient Materials

Kits available for two, five, or fifteen 96-well plates*

Specificity

Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure total Survivin in cell lysates. An immobilized capture antibody specific for Survivin binds both phosphorylated and unphosphorylated Survivin. After washing away unbound material, a biotinylated detection antibody is used to detect both phosphorylated and unphosphorylated protein, utilizing a standard Streptavidin-HRP format.

Product Features

Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
Development protocols are provided to guide further assay optimization
Assay can be customized to your specific needs
Available in 2, 5, and 15- (96-well) plate pack sizes
Economical alternative to Western blot

Kit Content

Capture Antibody
Conjugated Detection Antibody
Calibrated Immunoassay Standard or Control
Streptavidin-HRP

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na₂HPO₄, 1.5 mM KH₂O₄, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Lysis Buffer*

IC Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H₂O₂) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H₂SO₄ (Catalog # DY994)

Microplates: From Costar EIA Plate (Costar Catalog # 2592) or R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product

Scientific Data

ELISA

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Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Survivin

Survivin is a member of the Inhibitor of Apoptosis (IAP) gene family and contains a single BIR domain. Increased survivin expression at the G2/M checkpoint contributes to tissue homeostasis and differentiation during embryonic and fetal development. Increased expression in differentiated adult tissues, however, frequently results in unchecked cell survival, resulting in cancer. Survivin can protect cells from caspase-induced apoptosis activated in mitosis presumably by binding to Caspase-3, -7, and -9.

Entrez Gene IDs:

332 (Human)

Alternate Names:

API4; apoptosis inhibitor 4; Apoptosis inhibitor survivin; baculoviral IAP repeat-containing 5; Baculoviral IAP Repeat-Containing Protein 5; BIRC5; EPR-1; IAP4; survivin variant 3 alpha; Survivin

Citations for Human Total Survivin DuoSet IC ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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Prognostic Significance of BIRC5/Survivin in Breast Cancer: Results from Three Independent Cohorts
Authors: N Oparina, MC Erlandsson, A Fäldt Bedi, T Parris, K Helou, P Karlsson, Z Einbeigi, MI Bokarewa
Cancers, 2021-05-04;13(9):.
Species: Human
Sample Types: Tissue Homogenates
Serum survivin predicts responses to treatment in active rheumatoid arthritis: a post hoc analysis from the SWEFOT trial.
Authors: Levitsky A, Erlandsson M, van Vollenhoven R, Bokarewa M
BMC Med, 2015-09-30;13(0):247.
Species: Human
Sample Types: Serum
Activation of Fms-like tyrosine kinase 3 signaling enhances survivin expression in a mouse model of rheumatoid arthritis.
Authors: Andersson S, Svensson M, Erlandsson M, Dehlin M, Andersson K, Bokarewa M
PLoS ONE, 2012-10-17;7(10):e47668.
Species: Human, Mouse
Sample Types: Cell Culture Supernates, Plasma
Serum levels of inhibitors of apoptotic proteins (IAPs) change with IVIg therapy in pemphigus.
Authors: Toosi, Siavash, Habib, Nancy, Torres, Geneviev, Reynolds, Sandra R, Bystryn, Jean-Cla
J Invest Dermatol, 2011-06-30;131(11):2327-9.
Species: Human
Sample Types: Serum
TEL-AML1 regulation of survivin and apoptosis via miRNA-494 and miRNA-320a.
Authors: Diakos C, Zhong S, Xiao Y, Zhou M, Vasconcelos GM, Krapf G, Yeh RF, Zheng S, Kang M, Wiencke JK, Pombo-de-Oliveira MS, Panzer-Grumayer R, Wiemels JL
Blood, 2010-08-31;116(23):4885-93.
Species: Human
Sample Types: Cell Lysates
SPC3042: a proapoptotic survivin inhibitor.
Authors: Hansen JB, Fisker N, Westergaard M, Kjaerulff LS, Hansen HF, Thrue CA, Rosenbohm C, Wissenbach M, Orum H, Koch T
Mol. Cancer Ther., 2008-09-01;7(9):2736-45.
Species: Human
Sample Types: Cell Lysates
Extracellular survivin up-regulates adhesion molecules on the surface of leukocytes changing their reactivity pattern.
Authors: Mera S, Magnusson M, Tarkowski A, Bokarewa M
J. Leukoc. Biol., 2007-10-15;83(1):149-55.
Species: Human
Sample Types: Plasma
Balance between survivin, a key member of the apoptosis inhibitor family, and its specific antibodies determines erosivity in rheumatoid arthritis.
Authors: Bokarewa M, Lindblad S, Bokarew D, Tarkowski A
Arthritis Res. Ther., 2005-01-21;7(2):R349-58.
Species: Human
Sample Types: Plasma
siRNA-mediated down-regulation of survivin inhibits bladder cancer cell growth.
Authors: Ning S, Fuessel S, Kotzsch M, Kraemer K, Kappler M, Schmidt U, Taubert H, Wirth MP, Meye A
Int. J. Oncol., 2004-10-01;25(4):1065-71.
Species: Human
Sample Types: Cell Lysates
Cyclooxygenase 2-dependent expression of survivin is critical for apoptosis resistance in non-small cell lung cancer.
Authors: Krysan K, Dalwadi H, Sharma S, Pold M, Dubinett S
Cancer Res., 2004-09-15;64(18):6359-62.
Species: Human
Sample Types: Cell Lysates

Systematic in vitro evaluation of survivin directed antisense oligodeoxynucleotides in bladder cancer cells.
Authors: Fuessel S, Kueppers B, Ning S, Kotzsch M, Kraemer K, Schmidt U, Meye A, Wirth MP
J. Urol., 2004-06-01;171(6):2471-6.
Species: Human
Sample Types: Cell Lysates

Elevated expression level of survivin protein in soft-tissue sarcomas is a strong independent predictor of survival.
Authors: Kappler M, Kotzsch M, Bartel F, Fussel S, Lautenschlager C, Schmidt U, Wurl P, Bache M, Schmidt H, Taubert H, Meye A
Clin. Cancer Res., 2003-03-01;9(3):1098-104.