Human TRAIL/TNFSF10 Alexa Fluor® 700-conjugated Antibody

Catalog #: FAB687N Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB687N-100UG

Detection of TRAIL/TNFSF10 in Human PBMCs by Flow Cytometry.

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Human TRAIL/TNFSF10 Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human TRAIL in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 75402

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TRAIL
Thr95-Gly281
Accession # P50591

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Label

Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25-1 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of TRAIL/TNFSF10 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated with 150 ng/mL IFN-a for 48 hours were stained with Mouse Anti-Human TRAIL/TNFSF10 Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB687N) and Mouse Anti-Human CD14 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB3832F). Quadrant markers were set based on control antibody staining (Catalog # IC002N). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: TRAIL/TNFSF10

TRAIL is a type II transmembrane protein belonging to the TNF superfamily and is now designated TNFSF10. TRAIL is active as a homotrimer and is produced by a variety of cell types in both the membrane bound form and also as a soluble molecule. It binds to any of the four TRAIL receptors as well as to Osteoprotegerin.

Long Name

TNF-related Apoptosis-inducing Ligand

Entrez Gene IDs

8743 (Human); 22035 (Mouse); 246775 (Rat)

Alternate Names

Apo-2 ligand; APO2L; Apo-2Ltumor necrosis factor (ligand) family, member 10; APO2Ltumor necrosis factor apoptosis-inducing ligand splice variant delta; CD253 antigen; CD253; Protein TRAIL; TL2; TNF-related apoptosis-inducing ligand; TNFSF10; TRAIL; TRAILTNF-related apoptosis inducing ligand TRAIL; tumor necrosis factor (ligand) superfamily, member 10; tumor necrosis factor ligand superfamily member 10

Product Datasheets

Product Datasheet

COA

SDS

Product Specific Notices

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.