Human TRAIL/TNFSF10 Quantikine ELISA Kit

R&D Systems | Catalog # DTRL00

R&D Systems
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Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL), Cell Lysates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL), Saliva (25 µL)

Sensitivity

7.87 pg/mL

Assay Range

15.6-1000 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Saliva)
View all TRAIL/TNFSF10 ELISA Kits »

Product Summary for Human TRAIL/TNFSF10 Quantikine ELISA Kit

The Quantikine Human TRAIL Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human TRAIL in cell culture supernates, serum, plasma, cell lysates, and saliva. It contains NS0-expressed recombinant human TRAIL and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human TRAIL showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human TRAIL

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Human

Specificity

Natural and recombinant human TRAIL

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity not tested.

Interference

Interference observed with 1 or more available related molecules.

精度

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, Cell Lysates, Saliva

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 95.0 193 391 96.0 191 388
Standard Deviation 4.26 8.81 24.3 8.30 14.8 22.2
CV% 4.5 4.6 6.2 8.6 7.7 5.7

EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 102 203 408 102 202 407
Standard Deviation 3.45 5.73 23.0 7.59 12.2 17.8
CV% 3.4 2.8 5.6 7.5 6.1 4.4

Recovery for Human TRAIL/TNFSF10 Quantikine ELISA Kit

The recovery of TRAIL spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 101 92-105
EDTA Plasma (n=4) 103 96-112
Heparin Plasma (n=4) 103 98-115
Serum (n=4) 96 91-102

Linearity

To assess the linearity of the assay, samples containing or spiked with high concentrations of TRAIL were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Human TRAIL/TNFSF10 ELISA Linearity

Scientific Data Images for Human TRAIL/TNFSF10 Quantikine ELISA Kit

Human TRAIL/TNFSF10 ELISA Cell Culture Supernate/Cell Lysate/Saliva Standard Curve

Human TRAIL/TNFSF10 ELISA Cell Culture Supernate/Cell Lysate/Saliva Standard Curve

Human TRAIL/TNFSF10 ELISA Serum/Plasma Standard Curve

Human TRAIL/TNFSF10 ELISA Serum/Plasma Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TRAIL/TNFSF10

TNF-related apoptosis-inducing ligand (TRAIL), also known as Apo-2 ligand and TNFSF10, is a type II transmembrane protein with a carboxy-terminal extracellular domain that exhibits homology to other TNF superfamily members. Among TNF superfamily members, TRAIL is the most homologous to Fas Ligand, sharing 28% amino acid sequence identity in their extracellular domains. Human TRAIL shares 65% amino acid sequence identity with mouse TRAIL and is active on mouse cells.

Long Name

TNF-related Apoptosis-inducing Ligand

Alternate Names

CD253, TNFSF10

Entrez Gene IDs

8743 (Human); 22035 (Mouse); 246775 (Rat)

Gene Symbol

TNFSF10

Additional TRAIL/TNFSF10 Products

Product Documents for Human TRAIL/TNFSF10 Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human TRAIL/TNFSF10 Quantikine ELISA Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human TRAIL/TNFSF10 Quantikine ELISA Kit

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  • Human TRAIL/TNFSF10 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Serum
    Verified Customer | Posted 11/15/2022
    Human TRAIL/TNFSF10 Quantikine ELISA Kit DTRL00
  • Human TRAIL/TNFSF10 Quantikine ELISA Kit
    Name: Adam Guess
    Sample Tested: Cell Culture Supernates
    Verified Customer | Posted 10/04/2017
    Human TRAIL/TNFSF10 Quantikine ELISA Kit DTRL00

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Protocols

View specific protocols for Human TRAIL/TNFSF10 Quantikine ELISA Kit (DTRL00):

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  7.   Aspirate and wash 4 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
    9. 50 µL Stop Solution
  9.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

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