Human TRAILR4/TNFRSF10D Antibody

Catalog #: AF633
11 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF633
AF633-SP

Human TRAILR4/TNFRSF10D ELISA Standard Curve.

2 Images

Product Details

Citations (11)

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TRAILR4/TNFRSF10D Antibody Summary

Species Reactivity

Human

Specificity

Detects human TRAIL R4/TNFRSF10D in direct ELISAs and Western blots. In Western blots, less than 5% cross‑reactivity with recombinant human (rh) TRAIL R1, rhTRAIL R2, and rhTRAIL R3 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TRAIL R4/TNFRSF10D
Ala56-His211
Accession # Q9UBN6

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human TRAIL R4/TNFRSF10D Fc Chimera (Catalog # 633-TR)

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human TRAILR4/TNFRSF10D Monoclonal Antibody (Catalog # MAB633R).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human TRAIL R4/TNFRSF10D DuoSet ELISA Kit (Catalog # DY633) for convenient development of a sandwich ELISA.

Flow Cytometry

2.5 µg/10⁶ cells

Human whole blood granulocytes

Immunohistochemistry

5-15 µg/mL

Immersion fixed paraffin-embedded sections of human brain (occipital cortex) subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Neutralization

Measured by its ability to neutralize TRAIL R4/TNFRSF10D-mediated inhibition of cytotoxicity in the L‑929 mouse fibroblast cell line. The Neutralization Dose (ND₅₀) is typically 0.03-0.1 µg/mL in the presence of 10 ng/mL Recombinant Human TRAIL R4/TNFRSF10D Fc Chimera.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

View Larger

Human TRAILR4/TNFRSF10D ELISA Standard Curve. Recombinant Human TRAILR4/TNFRSF10D protein was serially diluted 2-fold and captured by Mouse Anti-Human TRAILR4/TNFRSF10D Monoclonal Antibody (MAB633R) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Human TRAILR4/TNFRSF10D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF633) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

Neutralization

View Larger

TRAIL R4/TNFRSF10D Inhibition of TRAIL/TNFSF10-induced Cytotoxicity and Neutralization by Human TRAIL R4/TNFRSF10D Antibody. In the presence of a cross-linking antibody, Mouse polyHistidine Monoclonal Antibody (5 µg/mL, Catalog # MAB050) and the metabolic inhibitor actinomycin D (1 µg/mL), Recombinant Human TRAIL R4/TNFRSF10D Fc Chimera (Catalog # 633-TR) inhibits Recombinant Human TRAIL/TNFSF10 (Catalog # 375-TL) induced cytotoxicity in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Under these conditions, inhibition of Recombinant Human TRAIL/TNFSF10 (12 ng/mL) activity elicited by Recombinant Human TRAIL R4/TNFRSF10D Fc Chimera (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human TRAIL R4/ TNFRSF10D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF633). The ND₅₀ is typically 0.03-0.1 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TRAILR4/TNFRSF10D

Human TRAIL R4, also called decoy receptor 2 (DcR2) and TRUNND (TRAIL receptor with a truncated death domain), is a type 1, TNF R family membrane protein, which is a receptor for TRAIL (APO2 ligand). In the new TNF superfamily nomenclature, TRAIL R4 is referred to as TNFRSF10D. TRAIL R4 is unique among the TRAIL receptors in that its cytoplasmic domain contains a truncated consensus death domain motif. Binding of TRAIL R4 does not result in an apoptotic signal. Overexpression of TRAIL R4 can protect cells bearing TRAIL R1 and/or TRAIL R2 from TRAIL-mediated apoptosis. The human soluble TRAIL R4/Fc chimera neutralizes the ability of TRAIL to induce apoptosis.

References

Griffith, T.S. et al. (1998) Curr. Opin. Immunol. 10:559.
Pan, G. et al. (1998) FEBS lett 424:41.
Marsters, S.A. et al. (1997) Cur. Biol. 7:1003.
Degli-Esposti, M.A. et al. (1997) Immunity 7:813.

Long Name

TRAIL Receptor 4

Entrez Gene IDs

8793 (Human)

Alternate Names

CD264 antigen; CD264; DcR2; DCR2decoy with truncated death domain; Decoy receptor 2; TNF receptor-related receptor for TRAIL; TNF-related apoptosis-inducing ligand receptor 4; TNFRSF10D; TRAIL R4; TRAILR4; TRAIL-R4; TRAILR4TRAIL receptor 4; TRUNDDTRAIL receptor with a truncated death domain; tumor necrosis factor receptor superfamily member 10D; tumor necrosis factor receptor superfamily, member 10d, decoy with truncateddeath domain

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human TRAILR4/TNFRSF10D Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
Filter your results:

Filter by:

Critical Role for Monocytes/Macrophages in Rapid Progression to AIDS in Pediatric Simian Immunodeficiency Virus-Infected Rhesus Macaques.
Authors: Sugimoto C, Merino KM, Hasegawa A, Wang X.
J. Virol.
Resistance to TRAIL in non-transformed cells is due to multiple redundant pathways
Authors: M van Dijk, A Halpin-McCormick, T Sessler, A Samali, E Szegezdi
Cell Death & Disease
Cytotoxic Efficacy and Resistance Mechanism of a TRAIL and VEGFA-Peptide Fusion Protein in Colorectal Cancer Models
Authors: M Kopczynski, M Statkiewic, M Cybulska, U Kuklinska, K Unrug-Biel, Z Sandowska-, A Grochowska, M Gajewska, M Kulecka, J Ostrowski, M Mikula
International Journal of Molecular Sciences, 2021-03-19;22(6):.
Species: Human
Sample Types: Whole Cells
Applications: ICC
Chemotherapeutic Agents Sensitize Resistant Cancer Cells to the DR5-Specific Variant DR5-B more Efficiently than to TRAIL by Modulating the Surface Expression of Death and Decoy Receptors
Authors: ?? ?rtykov, DA Belov, VO Shipunova, DB Trushina, SM Deyev, DA Dolgikh, MP Kirpichnik, ME Gasparian
Cancers (Basel), 2020-04-30;12(5):.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture
Dual role of DR5 in death and survival signaling leads to TRAIL resistance in cancer cells
Authors: Y Shlyakhtin, V Pavet, H Gronemeyer
Cell Death Dis, 2017-08-31;8(8):e3025.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation
Tumor necrosis factor-related apoptosis-inducing ligand promotes migration of human bone marrow multipotent stromal cells.
Authors: Secchiero P, Melloni E, Corallini F, Beltrami AP, Alviano F, Milani D, D'Aurizio F, di Iasio MG, Cesselli D, Bagnara GP, Zauli G
Stem Cells, 2008-09-04;26(11):2955-63.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
Differential response of p53 and p21 on HDAC inhibitor-mediated apoptosis in HCT116 colon cancer cells in vitro and in vivo.
Authors: Zopf S, Neureiter D, Bouralexis S, Abt T, Glaser KB, Okamoto K, Ganslmayer M, Hahn EG, Herold C, Ocker M
Int. J. Oncol., 2007-12-01;31(6):1391-402.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Acquired resistance to TRAIL-induced apoptosis in human ovarian cancer cells is conferred by increased turnover of mature caspase-3.
Authors: Lane D, Cote M, Grondin R, Couture MC, Piche A
Mol. Cancer Ther., 2006-03-01;5(3):509-21.
Species: Human
Sample Types: Whole Cells
Applications: Western Blot
Death receptors, Fas and TRAIL receptors, are involved in human osteoclast apoptosis.
Authors: Roux S, Lambert-Comeau P, Saint-Pierre C, Lepine M, Sawan B, Parent JL
Biochem. Biophys. Res. Commun., 2005-07-22;333(1):42-50.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P
Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) induces rheumatoid arthritis synovial fibroblast proliferation through mitogen-activated protein kinases and phosphatidylinositol 3-kinase/Akt.
Authors: Morel J, Audo R, Hahne M, Combe B
J. Biol. Chem., 2005-01-31;280(16):15709-18.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) sequentially upregulates nitric oxide and prostanoid production in primary human endothelial cells.
Authors: Zauli G, Pandolfi A, Gonelli A, Di Pietro R, Guarnieri S, Ciabattoni G, Rana R, Vitale M, Secchiero P
Circ. Res., 2003-03-20;92(7):732-40.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry