Human Transferrin Antibody

Catalog #: MAB5746
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5746
MAB5746-SP

Detection of Human Transferrin by Western Blot.

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All Transferrin Antibodies

Product Details

Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Transferrin Antibody Summary

Species Reactivity

Human

Specificity

Detects human Transferrin in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG₁ Clone # 507506

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Transferrin
Val20-Pro698
Accession # AAH59367

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Transferrin by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line (negative control) and human liver. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Transferrin Monoclonal Antibody (Catalog # MAB5746) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Transferrin at approximately 67-70 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Western Blot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Transferrin

Transferrin, also known as serotransferrin and siderophilin, is an 80 kDa secreted glycoprotein that binds two Fe (3+) ions, transporting them from sites of absorption and heme degradation to those of storage and utilization. It is a member of the Transferrin family of proteins. Human Transferrin is synthesized as a 698 amino acid (aa) precursor that contains a 19 aa signal sequence and a 679 aa mature chain. The mature chain consists of two Transferrin-like domains, two high-affinity iron binding sites, one potential site for O-linked glycosylation, and two potential sites for N-linked glycosylation. Human Transferrin is 73% aa identical to mouse and rat Transferrin.

Entrez Gene IDs

7018 (Human)

Alternate Names

Beta-1 metal-binding globulin; DKFZp781D0156; EC 3.4.21; PRO1557; PRO2086; Serotransferrin; Siderophilin; TF; Transferrin

Product Datasheets

Product Datasheet

COA

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Citations for Human Transferrin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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