Human TRIM Antibody

Catalog #: MAB4708 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4708
MAB4708-SP

Detection of TRIM in Human CD3+Lymphocytes by Flow Cytometry.

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All TRIM Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TRIM Antibody Summary

Species Reactivity

Human

Specificity

Detects human TRIM in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG₁ Clone # 488806

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human TRIM
Thr50-Asn186
Accession # Q6PIZ9

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human TRIM

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/10⁶ cells

Human blood‑derived CD3⁺ lymphocytes fixed with paraformaldehyde and permeabilized with saponin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of TRIM antibody in Human CD3<SUP>+</SUP>Lymphocytes antibody by Flow Cytometry.

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Detection of TRIM in Human CD3⁺Lymphocytes by Flow Cytometry. Human blood-derived CD3⁺lymphocytes were stained with Mouse Anti-Human TRIM Monoclonal Antibody (Catalog # MAB4708, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0101B). To facilitate intra-cellular staining, cells were fixed with paraformaldehyde and permea-bilized with saponin.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TRIM

Human TRIM (T cell receptor-interacting molecule) (also trat1, T cell receptor-associated transmembrane adaptor 1, and pp29/30) is a 30 kDa, type III transmembrane protein, that is a member of the transmembrane adaptor protein (TRAP) family. It contains a short, 8 aa extracellular region, a 19 aa transmembrane region, and a 159 aa cytosolic tail. Its cytoplasmic tail contains several tyrosine motifs with the potential to bind to Src-homology 2 (SH2) domains of signaling proteins. TRIM is present in T cells and NK cells. Human TRIM shares 66% aa sequence identity with mouse TRIM.

Long Name

T Cell Receptor Interacting Molecule

Entrez Gene IDs

50852 (Human); 77647 (Mouse); 498075 (Rat)

Alternate Names

pp29/30; T cell receptor associated transmembrane adaptor 1; T cell receptor interacting molecule; T-cell receptor interacting molecule; T-cell receptor-associated transmembrane adapter 1; T-cell receptor-interacting molecule; TCRIM; TRAT1; TRIM; TRIMHSPC062