What is the light chain of Human TRIM21 Antibody, Catalog # MAB6219?

Name: Human TRIM21 Antibody
Brand: R&D Systems
SKU: MAB6219
Price: 429 USD

Human TRIM21 Antibody

Catalog #: MAB6219 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6219
MAB6219-SP

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Human TRIM21 Antibody Summary

Species Reactivity

Human

Specificity

Detects human TRIM21 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) TRIM, rhTRIM5, rhTRIM5a, rhTRIM32, rhTRIF (aa 29-204), or rhTRIF (aa 474-618) is observed

Source

Monoclonal Mouse IgG₁ Clone # 672722

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human TRIM21
Arg195-Pro293
Accession # P19474

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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TRIM21 in Human PBMCs. TRIM21 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated for 8 hours with 20 ng/mL of Recombinant Human IFN-gamma (Catalog # 285-IF) using Mouse Anti-Human TRIM21 Monoclonal Antibody (Catalog # MAB6219) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # NL009) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TRIM21

TRIM21 (Tripartite motif-containing protein 21; also Ro(SS-A), 52 kDa Ro Protein/Ro52, and RING finger protein 81) is a 52-56 kDa member of the RING finger-B-box-coiled-coil family of proteins. It is an E3 ligase that is found in both nucleus and cytoplasm, where it is often associated with microtubules. TRIM21 ubiquitinates select proteins. In B cells, it targets the Fc fragment of misfolded IgG, providing QC on its production. In macrophages, it acts in a nondegradative manner on IRF8, promoting innate immunity. Human TRIM21 is 475 amino acids (aa) in length. It contains one E3 ligase RING finger domain (aa 16-55), a B-Box type zinc-finger region (aa 92-123), a coiled-coil region (aa 128-238) and a C-terminal SPRY/B30.2 Ig binding domain (aa 268-465). TRIM21 is reported to form trimers. Over aa 195‑293, human TRIM21 exhibits 72% aa identity with mouse TRIM21.

Long Name

Tripartite Motif-containing Protein 21

Entrez Gene IDs

6737 (Human); 20821 (Mouse); 308901 (Rat)

Alternate Names

52 kDa ribonucleoprotein autoantigen Ro/SS-A; 52 kDa Ro/SSA; E3 ubiquitin-protein ligase TRIM21; EC 6.3.2.-; rhTRIM21; RNF81; RNF81RING finger protein 81; ro(SS-A); Ro52; RO52SS-A; Sicca syndrome antigen A; Sjoegren syndrome type A antigen; Sjogren syndrome antigen A1 (52kDa, ribonucleoprotein autoantigen SS-A/Ro); SSA1; SSA1SSA; TRIM21; tripartite motif containing 21,52 kDa Ro protein; tripartite motif-containing 21; Tripartite motif-containing protein 21