Human TRIM32 Antibody

Catalog #: MAB6515 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6515
MAB6515-SP

Detection of Human TRIM32 by Western Blot.

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Human TRIM32 Antibody Summary

Species Reactivity

Human

Specificity

Detects human TRIM32 in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG₁ Clone # 717801

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human TRIM32
Arg105-Lys204
Accession # Q13049

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human TRIM32 by Western Blot. Western blot shows lysates of A172 human glioblastoma cell line, DU145 human prostate carcinoma cell line, and PC-3 human prostate cancer cell line. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human TRIM32 Monoclonal Antibody (Catalog # MAB6515) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for TRIM32 at approximately 72 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TRIM32

TRIM32 (Tripartite motif-containing protein 32; also 72 kDa TAT-interacting protein, and zinc finger protein HT2A) is a 72-82 kDa member of the TRIM/RBCC family of proteins. It is an E3 ligase that is found in structures termed nuclear and cytoplasmic bodies. Cells known to express TRIM32 are diverse and include fibroblasts, keratinocytes, skeletal muscle cells and neurons. TRIM32 ubiquitinates select proteins such as c-myc, Abi2, actin and dysbindin. Human TRIM32 is 653 amino acids (aa) in length. It contains one E3 ligase RING finger domain (aa 20-65), a B-Box type zinc-finger region (aa 103-133), a coiled-coil region (aa 138-197), five NHL repeats (aa 358-646) and three utilized phosphorylation sites (Ser328/335/339). TRIM32 has the potential to form homomultimers. Over aa 105-204, human TRIM32 exhibits 95% aa identity with mouse TRIM32.

Long Name

Tripartite Motif-containing Protein 32

Entrez Gene IDs

22954 (Human); 69807 (Mouse); 313264 (Rat)

Alternate Names

72 kDa Tat-interacting protein; BBS11; BBS1172-KD; EC 6.3.2.-; HT2A; HT2Alimb girdle muscular dystrophy 2H (autosomal recessive); LGMD2H; TATIP; TRIM32; tripartite motif containing 32; Zinc finger protein HT2A; zinc-finger protein HT2A