Human TRIM5 Antibody

Catalog #: AF3684 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3684
AF3684-SP

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TRIM5 Antibody Summary

Species Reactivity

Human

Specificity

Detects human TRIM5 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse TRIM5 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human TRIM5
Ala2-Gln248
Accession # Q9C035

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human TRIM5

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TRIM5

TRIM5 belongs to the large family of TRIM proteins, which are also named RBCC proteins. These proteins contain a RING domain, one or two B-boxes (CHC3H2 zinc finger motifs), and the coiled-coil domain. Different TRIM proteins contain C-terminals that vary in length and composition. They also have different functions and cellular localization. TRIM5 possesses a B30.2 (also known as RFP-like or PRY-SPRY) C-terminal domain and exists as a homotrimer. TRIM5 is a single protein RING finger E3 ubiquitin ligase and has been shown to have antiretroviral activities. Five splice variants that have identical amino-terminal residues 1‑248, but differ in their C-terminal regions have been reported. Human TRIM5 shares 49% amino acid sequence homology with mouse TRIM5.

Long Name

Tripartite Motif-containing 5

Entrez Gene IDs

85363 (Human)

Alternate Names

EC 6.3.2; EC 6.3.2.-; RING finger protein 88; RNF88; RNF88tripartite motif protein TRIM; TRIM5; TRIM5alpha; tripartite motif containing 5; tripartite motif protein TRIM5; tripartite motif-containing 5; tripartite motif-containing protein 5