Human TrkA Antibody

Catalog #: MAB1751R Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB1751R-100
MAB1751R-SP

Detection of TrkA in BAF Cell Line Transfected with Human TrkA and eGFP by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TrkA Antibody Summary

Species Reactivity

Human

Specificity

Detects human TrkA in direct ELISAs.

Source

Recombinant Monoclonal Mouse IgG₁ Clone # 165131R

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TrkA
Ala33-Glu407
Accession # P04629

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Knockout Validated

TrkA is specifically detected in K562 human chronic myelogenous leukemia cell line parental cell line but is not detectable in TrkA knockout K562 cell line.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of TrkA in BAF Cell Line Transfected with Human TrkA and eGFP by Flow Cytometry. BAF cell line transfected with human TrkA and eGFP was stained with (A) Mouse Anti-Human TrkA Monoclonal Antibody (Catalog # MAB1751R) or (B) Mouse IgG1 isotype control antibody (Catalog # MAB002) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry

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Detection of TrkA in K562 Human Cell Line by Flow Cytometry. K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human TrkA Monoclonal Antibody (Catalog # MAB1751R, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Knockout Validated

TrkA is specifically detected antibody in K562 human chronic myelogenous leukemia cell line parental cell line but is not detectable antibody in TrkA knockout K562 cell line.

View Larger

TrkA is specifically detected in K562 human chronic myelogenous leukemia cell line parental cell line but is not detectable in TrkA knockout K562 cell line. TrkA knockout K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human TrkA Monoclonal Antibody (Catalog # MAB1751R, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). No staining in the TrkA knockout K562 cell line was observed. View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TrkA

TrkA, the product of the proto-oncogene trk, is a member of the neurotrophic tyrosine kinase receptor family that has three members. TrkA, TrkB, and TrkC preferentially bind NGF, NT-4 and BDNF, and NT-3, respectively. All Trk family proteins share a conserved complex subdomain organization consisting of a signal peptide, two cysteine-rich domains, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains in the extracellular region, as well as an intracellular region that contains the tyrosine kinase domain. Two distinct TrkA isoforms that differ by virtue of a 6-amino acid insertion in their extracellular domain have been identified. The longer TrkA isoform is the only isoform expressed within neuronal tissues whereas the shorter TrkA is expressed mainly in non-neuronal tissues. NGF binds to TrkA with low affinity and activates its cytoplasmic kinase, initiating a signaling cascade that mediates neuronal survival and differentiation. Higher affinity binding of NGF requires the coexpression of TrkA with the p75 NGF receptor (NGF R), a member of the tumor necrosis factor receptor superfamily. NGF R binds all neurotrophins with low affinity and modulates Trk activity as well as alters the specificity of Trk receptors for their ligands. NGF R can also mediate cell death when expressed independent of Trk.

References

Esposito, D. et al. (2001) J. Biol. Chem. 276:32687.
Sofroniew, M.V. et al. (200) Annu. Rev. Neurosci. 24:1217.

Long Name

Neurotrophic Tyrosine Kinase Receptor A

Entrez Gene IDs

4914 (Human); 18211 (Mouse); 59109 (Rat)

Alternate Names

DKFZp781I14186; EC 2.7.10; EC 2.7.10.1; MTChigh affinity nerve growth factor receptor; Neurotrophic tyrosine kinase receptor type 1; neurotrophic tyrosine kinase, receptor, type 1; NTRK1; NTRK-1; p140-TrkA; TRK1-transforming tyrosine kinase protein; TrkA; Trk-A; TRKAOncogene TRK; TRKTRK1; tyrosine kinase receptor A