Human TrkB Antibody Summary
Cys32-His430
Accession # Q16620
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of TrkB in Baf3 cells by Flow Cytometry. Baf3 cells transfected with Human TrkB (filled histogram) vs Irrelevant transfectant cells (open histogram) were stained with Mouse Anti-Human TrkB Monoclonal Antibody (Catalog # MAB3971) followed by Fluorescein-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0103B). View our protocol for Staining Membrane-associated Proteins.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TrkB
The neurotrophins, including NGF, BDNF, NT-3, and NT-4/5 constitute a group of structurally related, secreted proteins that play an important role in the development and function of the nervous system. The biological activities of the neurotrophins are mediated by binding to the different members of the Trk family tyrosine kinase receptors. Three Trk family proteins, TrkA, TrkB, and TrkC, exhibiting different ligand specificities, have been identified. TrkA binds NGF, TrkB binds BDNF and
NT‑4/5 and TrkC binds NT-3. All Trk family proteins share a conserved complex subdomain organization consisting of a signal peptide, two cysteine-rich domains, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains in the extracellular region, as well as an intracellular region that contains the tyrosine kinase domain. Natural splice variants of the different Trks, including TrkB variants lacking the first cysteine-rich domain, the first and second or all three of the leucine-rich motifs, or the tyrosine kinase domain, have been described. The role of the different extracellular subdomains of TrkB in mediating neurotrophin binding and discrimination is currently being investigated. At the protein sequence level, human and rat TrkB are greater than 90% identical and the proteins exhibit cross-species activity. TrkB is primarily expressed in the nervous system. However, low levels of TrkB expression have also been observed in a wide variety of tissues (pancreas, kidneys, ovary) outside the nervous system.
- Ninkina, N. et al. (1997) J. Biol. Chem. 272:13019.
- Middlemas, D.S. et al. (1991) Mol. Cell Biol. 11:143.
- Soppet, D. et al. (1991) Cell 65:895.
Product Datasheets
Citations for Human TrkB Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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A protocol to differentiate nociceptors, mechanoreceptors, and proprioceptors from human pluripotent stem cells
Authors: Kenyi Saito-Diaz, Nadja Zeltner
STAR Protocols
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Pharmaceutical Characterization of Tropomyosin Receptor Kinase B-Agonistic Antibodies on Human Induced Pluripotent Stem (hiPS) Cell–Derived Neurons
Authors: Stefanie Traub, Heiko Stahl, Holger Rosenbrock, Eric Simon, Lore Florin, Lisa Hospach et al.
Journal of Pharmacology and Experimental Therapeutics
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Isolation of human pluripotent stem cell-derived sensory neuron subtypes by immunopanning
Authors: Kenyi Saito-Diaz, Christina James, Archie Jayesh Patel, Nadja Zeltner
Frontiers in Cell and Developmental Biology
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Multiplex coherent anti-Stokes Raman scattering microspectroscopy detection of lipid droplets in cancer cells expressing TrkB
Authors: T Guerenne-D, V Couderc, L Duponchel, V Sol, P Leproux, JM Petit
Sci Rep, 2020-10-07;10(1):16749.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
P75 neurotrophin receptor is sequestered in the Golgi apparatus of the U-87 MG human glioblastoma cell line.
Authors: Giraud S, Loum E, Bessette B, Mathonnet M, Lalloue F
Int. J. Oncol., 2010-12-06;38(2):391-9.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
BDNF and its receptors in human myasthenic thymus: implications for cell fate in thymic pathology.
Authors: Berzi A, Ayata CK, Cavalcante P, Falcone C, Candiago E, Motta T, Bernasconi P, Hohlfeld R, Mantegazza R, Meinl E, Farina C
J. Neuroimmunol., 2008-06-13;197(2):128-39.
Species: Human
Sample Types: Whole Cells, Whole Tissue
Applications: Flow Cytometry, ICC, IHC-Fr -
Brain-derived neurotrophic factor stimulates bone/cementum-related protein gene expression in cementoblasts.
Authors: Kajiya M, Shiba H, Fujita T, Ouhara K, Takeda K, Mizuno N, Kawaguchi H, Kitagawa M, Takata T, Tsuji K, Kurihara H
J. Biol. Chem., 2008-04-03;283(23):16259-67.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Tissue-Specificity of Antibodies Raised Against TrkB and p75NTR Receptors; Implications for Platelets as Models of Neurodegenerative Diseases
Authors: Fleury S, Boukhatem I, Le Blanc J et al.
Frontiers in Immunology
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Neurotensin receptor type 2 protects B-cell chronic lymphocytic leukemia cells from apoptosis
Authors: A Abbaci, H Talbot, S Saada, N Gachard, J Abraham, A Jaccard et al.
Oncogene
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Neurogenin 3 is regulated by neurotrophic tyrosine kinase receptor type 2 (TRKB) signaling in the adult human exocrine pancreas
Authors: Michael J. Shamblott, Marci L. O’Driscoll, Danielle L. Gomez, Dustin L. McGuire
Cell Communication and Signaling
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