Human Tryptase alpha/beta-1 Antibody

Catalog #: MAB3796 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3796
MAB3796-SP

Detection of Tryptase alpha/beta-1 in Human Lung.

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Human Tryptase alpha/beta-1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Tryptase alpha/beta-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 50‑100% cross-reactivity with recombinant human (rh) TPSAB1 and recombinant mouse (rm) Mcpt6 is observed, 20‑100% cross-reactivity with rmMcpt7 is observed, and no cross-reactivity with rhTPSG1, rmTPS5, rmMcpt1, or rmMcpt11 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 349406

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Tryptase alpha/beta-1
Ile31-Pro275
Accession # AAD13876

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Tryptase alpha/beta-1 (Catalog # 3796-SE)

Immunohistochemistry

0.1-15 µg/mL

Immersion fixed paraffin-embedded sections of Human Lung

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human Tryptase alpha/beta-1 (Catalog # 3796‑SE), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Detection of Tryptase alpha/beta-1 in Human Lung. Tryptase alpha/beta-1 was detected in immersion fixed paraffin-embedded sections of Human Lung using Mouse Anti-Human Tryptase alpha/beta-1 Monoclonal Antibody (Catalog # MAB3796) at 0.1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to mast cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Tryptase alpha/beta-1

Tryptases are trypsin-like serine proteases, and beta tryptases appear to be the main isoenzymes expressed in mast cells (1). They are stored in secretory granules of mast cells, where they form active tetramers with heparin proteoglycan. Because of the unique arrangement of the active sites in the tetramer, which are facing a narrow central pore, beta tryptases are resistant to macromolecule protease inhibitors (2). When mast cells are activated, beta tryptases are released along with other proteins in secretory granules, participating in provoking inflammatory conditions (3). beta tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic disorders.

References

Caughey, G. H. 2004, in Handbook of Proteolytic Enzymes, Barrett, A.J. et al. eds. pp. 1535.
Sommerhoff, C.P. et al. (1999) Proc. Natl. Acad. Sci. USA. 96:10984.
Hallgren, J. and G. Pejler (2006) FEBS J. 273:1871.

Entrez Gene IDs

7177 (Human); 100503895 (Mouse); 54271 (Rat)

Alternate Names

EC 3.4.21; EC 3.4.21.59; mast cell tryptase beta II; mast cell tryptase beta III; TPS2; TPS2tryptase beta-2; TPSB2; Tryptase alpha/beta-1; tryptase beta 2 (gene/pseudogene); tryptase beta 2; tryptase beta II; tryptase beta III; Tryptase beta2; Tryptase beta-2; Tryptase II; tryptase III; tryptase-2; tryptaseB; tryptaseC; Tryptase-C