Human TSHR Antibody

Catalog #: MAB6534 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6534
MAB6534-SP

Detection of Human TSH R by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TSHR Antibody Summary

Species Reactivity

Human

Specificity

Detects human TSH R in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG_2A Clone # 484405

Purification

Protein A or G purified from ascites

Immunogen

NS0 mouse myeloma cell line transfected with human TSH R
Accession # P16473

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human TSH R Monoclonal Antibody (Catalog # MAB65341).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human TSH R by Western Blot. Western blot shows lysates of human thyroid tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human TSH R Monoclonal Antibody (Catalog # MAB6534) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for TSH R at approximately 55 and 83 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 7.

ELISA

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Human TSH R ELISA Standard Curve. Recombinant human TSH R protein was serially diluted 2-fold and captured by Mouse Anti-Human TSH R Monoclonal Antibody (Catalog # MAB65341) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human TSH R Monoclonal Antibody (Catalog # MAB6534) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TSHR

Thyroid stimulating hormone receptor (TSH R; also thyrotropin receptor) is an 85 kDa (unglycosylated) member of the G-protein coupled receptor 1 family. Human TSH R is synthesized as a 764 amino acid (aa) precursor that contains a 20 aa signal sequence, a 393 aa extracellular domain (ECD), a 269 aa membrane spanning domain consisting of seven transmembrane segments, and an 82 aa cytoplasmic domain. The ECD contains six leucine-rich repeats (LRR) and six potential sites of N-linked glycosylation. There are two isoforms, long and short, produced by a splicing variant corresponding to aa 253-764 in the long isoform that is missing in the short isoform. Human TSH R is 86-87% aa identical to mouse and rat TSH R. TSH R is the receptor for thyroid stimulating hormone, which plays a central role in controlling thyroid cell metabolism.

Long Name

Thyroid Stimulating Hormone Receptor

Entrez Gene IDs

7253 (Human); 22095 (Mouse)

Alternate Names

CHNG1; LGR3; LGR3hTSHR-I; MGC75129; seven transmembrane helix receptor; thyroid stimulating hormone receptor; Thyroid-stimulating hormone receptor; thyrotropin receptor; thyrotropin receptor-I, hTSHR-I; TSH R; TSHR; TSH-R