Home / TSPAN33 / Human TSPAN33 PE-conjugated Antibody

Human TSPAN33 PE-conjugated Antibody

Catalog #: FAB8405P
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB8405P-025
Detection of TSPAN33 in Human PBMCs by Flow Cytometry.
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Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TSPAN33 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects HEK293 human embryonic kidney cell line transfected with human TSPAN33 by Flow Cytometry. Does not detect untransfected or irrelevant transfected HEK293 cells.
Source
Monoclonal Mouse IgG2B Clone # 545422
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human TSPAN33
Met1-Tyr283
Accession # NP_848657
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of TSPAN33 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of TSPAN33 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with overnight with Recombinant Human IL-4 (Catalog # 204-IL) and Recombinant Human CD40 Ligand/TNFSF5 (Catalog # 6245-CL) for were stained with Mouse Anti-Human CD19 APC-conjugated Monoclonal Antibody (Catalog # FAB4867A) and either (A) Mouse Anti-Human TSPAN33 PE-conjugated Monoclonal Antibody (Catalog # FAB8405P) or (B) Mouse IgG2BPhycoerythrin Isotype Control (Catalog # IC0041P). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TSPAN33

TSPAN33 is a homodimeric disulfide-linked member of the tetraspanin family that is predominantly expressed in erythroblasts. It plays an important role in normal erythropoiesis and in the differentiation of erythroid progenitor and is expressed in several lymphomas including Hodgkin’s and Diffuse large B cell lymphoma and is expressed in some autoimmune diseases where B cells participate in the pathology. Full-length human TSPAN33 shares 96% amino acid identity with mouse TSPAN33.

Long Name
Tetraspanin 33
Entrez Gene IDs
340348 (Human); 232670 (Mouse); 500065 (Rat)
Alternate Names
hPen; PEN; Penumbra; proerythroblast new membrane; tetraspanin 33; tetraspanin-33; TSPAN33; tspan-33

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Citations for Human TSPAN33 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Single-Cell RNA Sequencing of Human, Macaque, and Mouse Testes Uncovers Conserved and Divergent Features of Mammalian Spermatogenesis
    Authors: Adrienne Niederriter Shami, Xianing Zheng, Sarah K. Munyoki, Qianyi Ma, Gabriel L. Manske, Christopher D. Green et al.
    Developmental Cell
  2. The adult human testis transcriptional cell atlas
    Authors: J Guo, EJ Grow, H Mlcochova, GJ Maher, C Lindskog, X Nie, Y Guo, Y Takei, J Yun, L Cai, R Kim, DT Carrell, A Goriely, JM Hotaling, BR Cairns
    Cell Res., 2018-10-12;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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