Human TSPAN9 Antibody

Catalog #: MAB6565 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6565
MAB6565-SP

Detection of Human TSPAN9 by Western Blot.

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Human TSPAN9 Antibody Summary

Species Reactivity

Human

Specificity

Detects human TSPAN9 in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG_2A Clone # 590409

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

HEK293 human embryonic kidney cell line transfected with human TSPAN9
Accession # O75954

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human TSPAN9 by Western Blot. Western blot shows lysates of human kidney tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human TSPAN9 Monoclonal Antibody (Catalog # MAB6565) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for TSPAN9 at approximately 27 to 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TSPAN9

Tetraspanin 9 (TSPAN9) is a 27 kDa glycoprotein and member of the tetraspanin (TM4SF) family of proteins. It is 239 amino acids (aa) in length and contains four transmembrane segments and one potential site for N-linked glycosylation. Both the N- and C-termini lie on the intracellular side of the membrane. Human TSPAN9 is 97% aa identical to mouse TSPAN9. It is expressed in megakaryocytes and platelets where it co-localizes with GP6 in tetraspanin microdomains on the platelet surface. TSPAN9 is involved in platelet activation.

Long Name

Tetraspanin 9

Entrez Gene IDs

10867 (Human)

Alternate Names

NET5; NET-5; NET5tetraspanin-9; new EST tetraspan 5; PP1057; Tetraspan NET-5; tetraspanin 9; transmembrane 4 superfamily member tetraspan NET-5; TSPAN9; tspan-9