Human TTF-1/NKX2-1 Antibody

Catalog #: MAB9458 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB9458-100
MAB9458-SP

Detection of Human TTF-1/NKX2-1 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TTF-1/NKX2-1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human TTF-1/NKX2-1 in direct ELISAs.

Source

Recombinant Monoclonal Rabbit IgG Clone # 2054B

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human TTF-1/NKX2-1
Ala40-Pro111
Accession # P43699

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunocytochemistry

3-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human TTF-1/NKX2-1 by Western Blot. Western blot shows lysates of TT human medullary thyroid cancer cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human TTF-1/NKX2-1 Monoclonal Antibody (Catalog # MAB9458) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for TTF-1/NKX2-1 at approximately 40-45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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TTF-1/NKX2-1 in A549 Human Cell Line. TTF-1/NKX2-1 was detected in immersion fixed A549 human lung carcinoma cell line using Rabbit Anti-Human TTF-1/NKX2-1 Monoclonal Antibody (Catalog # MAB9458) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TTF-1/NKX2-1

NK2 homeobox 1 (NKX2-1), also known as thyroid transcription factor 1 (TTF-1), is a 371 amino acids (aa) protein encoded by the NKX2-1 gene. Expression of NKX2-1 transcription factor is the earliest indication of the establishment of respiratory progenitors as well as thyroid epithelium in the ventral foregut endoderm. NKX2-1 is critical for the expression of many pulmonary specific genes, including surfactant proteins SP-A, -B, and –C, as well as thyroid specific genes that are important in the maintenance of the thyroid differentiation phenotype.

Long Name

NK2 Homeobox 1

Entrez Gene IDs

7080 (Human); 21869 (Mouse); 25628 (Rat)

Alternate Names

BCH; benign chorea; BHC; Homeobox protein NK-2 homolog A; homeobox protein Nkx-2.1; NK2 homeobox 1; NK-2 homolog A; NKX2.1; NKX2-1; NKX2A; NKX2ANKX2.1; T/EBP; Thyroid nuclear factor 1; Thyroid transcription factor 1BHC; TITF1; TITF1TEBP; TTF1; TTF-1; TTF1BCH; TTF-1NK-2