Human Twist-1 Antibody

Catalog #: AF6230
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6230
AF6230-SP

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All Twist-1 Antibodies

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Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Twist-1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Twist-1 in direct ELISAs. In direct ELISAs, less than 16% cross-reactivity with recombinant human Twist-2 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Twist-1
Met1-His202
Accession # Q15672

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

5-15 µg/mL

See below

Intracellular Staining by Flow Cytometry

2.5 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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Twist‑1 in A549 Human Cell Line. Twist-1 was detected in immersion fixed A549 human lung carcinoma cell line treated with 10ng/mL of Recombinant Human TGF beta 1 (Catalog # 240-B) for 48 hours using Sheep Anti-Human Twist-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6230) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (yellow, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Intracellular Staining by Flow Cytometry

Detection of Twist-1 antibody in HeLa Human Cell Line antibody by Flow Cytometry.

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Detection of Twist‑1 in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Sheep Anti-Human Twist-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6230, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 493-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL012). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Twist-1

Twist-1 (Twist-related protein 1; also H-Twist and bHLHa38) is a 25‑27 kDa class B member of the bHLH transcription factor family of proteins. It is widely expressed in embryo, and select adult cells such as white adipocytes. In fat, Twist-1 induces fatty acid oxidation via PGC-1 alpha and CPT-1, and promotes MCP-1 and TNF-alpha secretion by adipocytes. In epithelium, Twist-1 dysregulation represses E-Cadherin and induces N-Cadherin expression, resulting in an epithelial-to-mesenchymal transition that can lead to cancer. Human Twist-1 is 202 amino acids (aa) in length. It contains a bHLH domain (aa 109-164) with an embedded DNA-binding motif. Twist-1 forms homodimers, and heterodimerizes with TCF3, HAND 1 and HAND 2. Two distinct mutations exist that may impact its dimerization pattern. There is one seven aa insertion after Ile135, and a second seven aa insertion after Pro139. A third unrelated variant shows a four Gly insert after Gly92. Full-length human Twist-1 shares 98% aa identity with mouse Twist-1.

Long Name

Twist Related Protein 1 [Drosophila]

Entrez Gene IDs

7291 (Human); 22160 (Mouse); 85489 (Rat)

Alternate Names

acrocephalosyndactyly 3; ACS3; B-HLH DNA binding protein; BHLHA38; bHLHa38BPES3; BPES3; Class A basic helix-loop-helix protein 38; CRS1; H-twist; H-twistblepharophimosis, epicanthus inversus and ptosis 3; SCSBPES2; twist homolog 1 (Drosophila); TWIST homolog of drosophila; TWIST; Twist1; Twist-1; twist-related protein 1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human Twist-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Rapid induction and long-term self-renewal of neural crest-derived ectodermal chondrogenic cells from hPSCs
Authors: Pei Shen, Lu Chen, Dahe Zhang, Simo Xia, Zhuman Lv, Duohong Zou et al.
npj Regenerative Medicine
Epithelial-mesenchymal transitioned circulating tumor cells capture for detecting tumor progression.
Authors: Satelli A, Mitra A, Brownlee Z, Xia X, Bellister S, Overman M, Kopetz S, Ellis L, Meng Q, Li S
Clin Cancer Res, 2014-12-16;21(4):899-906.
Species: Human
Sample Types: Whole Cells
Applications: ICC