Human Twist-1 Antibody

Catalog #: AF62301
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF62301
AF62301-SP

Detection of Human Twist‑1 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Twist-1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Twist-1 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant Twist-2 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli‑derived recombinant human Twist-1
Met1-His202
Accession # Q15672

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Twist‑1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and NCI-N87 human gastric carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human Twist-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF62301) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Twist-1 at approximately 24 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Twist-1

Twist-1 (Twist-related protein 1; also H-Twist and bHLHa38) is a 24‑27 kDa class B member of the bHLH transcription factor family of proteins. It is widely expressed in embryo, and select adult cells such as white adipocytes. In fat, Twist-1 induces fatty acid oxidation via PGC-1 alpha and CPT-1, and promotes MCP-1 and TNF-alpha secretion by adipocytes. In epithelium, Twist-1 dysregulation represses E-Cadherin and induces N-Cadherin expression, resulting in an epithelial-to-mesenchymal transition that can lead to cancer. Human Twist-1 is 202 amino acids (aa) in length. It contains a bHLH domain (aa 109-164) with an embedded DNA-binding motif. Twist-1 forms homodimers, and heterodimerizes with TCF3, HAND 1 and HAND 2. Two distinct mutations exist that may impact its dimerization pattern. There is one seven aa insertion after Ile135, and a second seven aa insertion after Pro139. A third unrelated variant shows a four Gly insert after Gly92. Full-length human Twist-1 shares 98% aa identity with mouse Twist-1.

Long Name

Twist Related Protein 1 [Drosophila]

Entrez Gene IDs

7291 (Human); 22160 (Mouse); 85489 (Rat)

Alternate Names

acrocephalosyndactyly 3; ACS3; B-HLH DNA binding protein; BHLHA38; bHLHa38BPES3; BPES3; Class A basic helix-loop-helix protein 38; CRS1; H-twist; H-twistblepharophimosis, epicanthus inversus and ptosis 3; SCSBPES2; twist homolog 1 (Drosophila); TWIST homolog of drosophila; TWIST; Twist1; Twist-1; twist-related protein 1

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Citation for Human Twist-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1