Human Tyk2 Antibody

Name: Human Tyk2 Antibody
Brand: R&D Systems
SKU: MAB3427
Rating: 3.5 (2 reviews)

Catalog #: MAB3427 2 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3427
MAB3427-SP

Detection of Human Tyk2 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Tyk2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Tyk2 in Western blots.

Source

Monoclonal Mouse IgG₃ Clone # 307214

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Tyk2
Pro2-Leu51
Accession # P29597

Formulation

Lyophilized from a 0.2 μm filtered solution in TBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Tyk2 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human Tyk2 Monoclonal Antibody (Catalog # MAB3427) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Tyk2 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Tyk2

Tyk2 belongs to the Janus family of intracellular tyrosine kinases that are activated by receptors for many cytokines. Activation of Tyk2 occurs via phosphorylation at two adjacent tyrosine residues, Y1054 and Y1055, within the kinase domain. Tyk2 is required for interferon beta -mediated tyrosine phosphorylation of STAT3 and interferon receptor 1. Mice lacking Tyk2 are susceptible to parasitic infection but resistant to autoimmune arthritis.

Long Name

Protein tyrosine kinase 2

Entrez Gene IDs

7297 (Human); 54721 (Mouse); 298706 (Rat)

Alternate Names

EC 2.7.10; EC 2.7.10.2; JTK1; non-receptor tyrosine-protein kinase TYK2; Tyk2; tyrosine kinase 2