Home / ULBP-3 / Human ULBP-3 PE-conjugated Antibody

Human ULBP-3 PE-conjugated Antibody

Catalog #: FAB1517P
20 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB1517P
Detection of ULBP‑3 in PC-3 Human Cell Line by Flow Cytometry.
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Citations (20)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ULBP-3 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human ULBP-3 in ELISAs. Does not cross-react with recombinant human (rh) ULBP-1 or rhULBP-2.
Source
Monoclonal Mouse IgG2A Clone # 166510
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
BaF3 mouse pro-B cell line transfected with human ULBP-3
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry View Larger

Detection of ULBP‑3 in PC-3 Human Cell Line by Flow Cytometry. PC-3 human prostate carcinoma cell line was stained with Mouse Anti-Human ULBP-3 PE-conjugated Monoclonal Antibody (Catalog # FAB1517P, filled histogram) or isotype control antibody (IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of ULBP‑3 antibody in Human monocyte-derived dendritic cells antibody by Flow Cytometry. View Larger

Detection of ULBP‑3 in Human monocyte-derived dendritic cells by Flow Cytometry. Human monocyte-derived dendritic cells were stained with Mouse Anti-Human ULBP-3 PE-conjugated Monoclonal Antibody (Catalog # FAB1517P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of ULBP-3 antibody in BaF3 Mouse Cell Line Transfected with Human ULBP-3 antibody by Flow Cytometry. View Larger

Detection of ULBP‑3 in BaF3 Mouse Cell Line Transfected with Human ULBP-3 by Flow Cytometry. BaF3 mouse pro-B cell line transfected with human ULBP-3 was stained with Mouse Anti-Human ULBP-3 PE-conjugated Monoclonal Antibody (Catalog # FAB1517P, filled histogram) or isotype control antibody (IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of Human ULBP-3 by Flow Cytometry View Larger

Detection of Human ULBP-3 by Flow Cytometry Histograms showing sample flow cytometry profiles for the ligands studied. Bars in each plot indicate the population expressing each aNKR, Gates were set using Unstained, single stained controls, fluorescence minus one, and secondary antibody alone controls. Image collected and cropped by CiteAb from the following publication (https://www.mdpi.com/1999-4915/9/10/295), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: ULBP-3

ULBP-3 is a member of a family of cell-surface proteins that function as ligands for human NKG2D. ULBP-3 has also been described under the names RaeT1N (retinoic acid early transcript), NKG2DL3, and ALCAN-gamma. The name ULBP-3 derives from the original identification of three proteins, ULBP-1, -2, and -3, as ligands for the human cytomegalovirus glycoprotein UL16; they were designated UL16 binding proteins (ULBP). The gene for ULBP-3 resides in a cluster of ten related genes, six of which encode potentially functional glycoproteins. Amino acid sequence identity within this family ranges from 30‑60%. These proteins are distantly related to MHC class I proteins, but they possess only the alpha 1 and alpha 2 Ig-like domains, and they have no capacity to bind peptide or interact with beta 2‑microglobulin. Some family members, including ULBP-3, are anchored to the membrane via a GPI-linkage, whereas others have transmembrane domains. ULBP-3 and several other family members are known to bind to human NKG2D, an activating receptor expressed on NK cells, NKT cells, gamma δ T cells, and CD8+ alpha beta T cells. Engagement of NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. The ULBPs are expressed on some tumor cells and have been implicated in tumor surveillance (1‑7).

References
  1. Cosman, D. et al. (2001) Immunity 14:123.
  2. Kubin, M. et al. (2001) Eur. J. Immunol. 31:1428.
  3. Sutherland, C. et al. (2002) J. Immunol. 168:671.
  4. Steinle, A. et al. (2001) Immunogenetics 53:279.
  5. Sutherland, C. et al. (2001) Immunol. Rev. 181:185.
  6. Pende, D. et al. (2002) Cancer Res. 62:6178.
  7. Radosavljevic, M. et al. (2002) Genomics 79:114.
  8. NKG2D and its Ligands (2002) http://www.RnDSystems.com/.
Long Name
UL16 Binding Protein-3
Entrez Gene IDs
79465 (Human)
Alternate Names
NKG2D ligand 3; RAET1N; UL16 binding protein 3; ULBP3; ULBP-3

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Citations for Human ULBP-3 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

20 Citations: Showing 1 - 10
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  1. Early TRAIL-engagement elicits potent multimodal targeting of melanoma by CD34+ progenitor cell-derived NK cells
    Authors: Amanda A. van Vliet, Ella Peters, Denise Vodegel, Daniëlle Steenmans, Monica Raimo, Susan Gibbs et al.
    iScience
  2. Antitumor effects of NK cells expanded by activation pre?processing of autologous feeder cells before irradiation in colorectal cancer
    Authors: Koh, EK;Lee, HR;Son, WC;Park, GY;Bae, J;Park, YS;
    Oncology letters
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. gammadelta T cells are effectors of immunotherapy in cancers with HLA class I defects
    Authors: NL de Vries, J van de Haa, V Veninga, M Chalabi, ME Ijsselstei, M van der Pl, J van den Bu, D Ruano, JG van den Be, JB Haanen, LJ Zeverijn, BS Geurts, GF de Wit, TW Battaglia, H Gelderblom, HMW Verheul, TN Schumacher, LFA Wessels, F Koning, NFCC de Miranda, EE Voest
    Nature, 2023-01-11;613(7945):743-750.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. NKp44-based chimeric antigen receptor effectively redirects primary T cells against synovial sarcoma
    Authors: Y Murayama, Y Kasahara, N Kubo, C Shin, M Imamura, N Oike, T Ariizumi, A Saitoh, M Baba, T Miyazaki, Y Suzuki, Y Ling, S Okuda, K Mihara, A Ogose, H Kawashima, C Imai
    Translational Oncology, 2022-08-20;25(0):101521.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Selection of tumor‑resistant variants following sustained natural killer cell‑mediated immune stress
    Authors: Thibault Carré, Jerome Thiery, Bassam Janji, Stéphane Terry, Gwendoline Gros, Guillaume Meurice et al.
    Oncology Reports
  6. Genetic and Molecular Basis of Heterogeneous NK Cell Responses against Acute Leukemia
    Authors: DR Makanga, F Da Rin de, G David, C Willem, L Dubreuil, N Legrand, T Guillaume, P Peterlin, A Lebourgeoi, MC Béné, A Garnier, P Chevallier, K Gendzekhad, A Cesbron, K Gagne, B Clemenceau, C Retière
    Cancers (Basel), 2020-07-16;12(7):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. iPSC-Derived Platelets Depleted of HLA Class I Are Inert to Anti-HLA Class I and Natural Killer Cell Immunity
    Authors: D Suzuki, C Flahou, N Yoshikawa, I Stirblyte, Y Hayashi, A Sawaguchi, M Akasaka, S Nakamura, N Higashi, H Xu, T Matsumoto, K Fujio, MG Manz, A Hotta, H Takizawa, K Eto, N Sugimoto
    Stem Cell Reports, 2019-12-26;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. The pharmalogical reactivation of p53 function improves breast tumor cell lysis by granzyme B and NK cells through induction of autophagy
    Authors: M Chollat-Na, T Ben Safta-, D Haferssas, G Meurice, S Chouaib, J Thiery
    Cell Death Dis, 2019-09-20;10(10):695.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Ex vivo-expanded highly purified natural killer cells in combination with temozolomide induce antitumor effects in human glioblastoma cells in vitro
    Authors: Y Tanaka, T Nakazawa, M Nakamura, F Nishimura, R Matsuda, K Omoto, Y Shida, T Murakami, I Nakagawa, Y Motoyama, H Morita, T Tsujimura, H Nakase
    PLoS ONE, 2019-03-06;14(3):e0212455.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Expression of ligands for activating natural killer cell receptors on cell lines commonly used to assess natural killer cell function
    Authors: A Tremblay-M, S Coenraads, Z Kiani, FP Dupuy, NF Bernard
    BMC Immunol., 2019-01-29;20(1):8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Overcoming Target Driven Fratricide for T Cell Therapy
    Authors: Eytan Breman, Benjamin Demoulin, Sophie Agaugué, Sebastien Mauën, Alexandre Michaux, Lorraine Springuel et al.
    Frontiers in Immunology
  12. Mesenchymal stromal cells of the bone marrow and natural killer cells: cell interactions and cross modulation
    Authors: M Najar, M Fayyad-Kaz, N Meuleman, D Bron, H Fayyad-Kaz, L Lagneaux
    J Cell Commun Signal, 2018-01-19;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Expression Profiles of Ligands for Activating Natural Killer Cell Receptors on HIV Infected and Uninfected CD4? T Cells
    Authors: A Tremblay-M, J Bruneau, B Lebouché, I Lisovsky, R Song, NF Bernard
    Viruses, 2017-10-12;9(10):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. Interleukin-15 stimulates natural killer cell-mediated killing of both human pancreatic cancer and stellate cells
    Authors: JRM Van Audena, J De Waele, E Marcq, J Van Loenho, E Lion, JMJ Van den Be, R Jesenofsky, A Masamune, G Roeyen, P Pauwels, F Lardon, M Peeters, ELJ Smits
    Oncotarget, 2017-01-01;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. T Cells Infiltrating Diseased Liver Express Ligands for the NKG2D Stress Surveillance System
    Authors: Wei-Chen Huang
    J. Immunol, 2016-12-28;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. Combination treatment with decitabine and ionizing radiation enhances tumor cells susceptibility of T cells
    Sci Rep, 2016-09-27;6(0):32470.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  17. Decitabine enhances anti-CD33 monoclonal antibody BI 836858-mediated natural killer ADCC against AML blasts
    Blood, 2016-03-24;127(23):2879-89.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  18. Mutual Interaction between Human Multipotent Adult Progenitor Cells and NK Cells
    Authors: Sandra A. Jacobs, Jeroen Plessers, Jef Pinxteren, Valerie D. Roobrouck, Catherine M. Verfaillie, Stefaan W. Van Gool
    Cell Transplantation
  19. Thermal- and Oxidative Stress Causes Enhanced Release of NKG2D Ligand-Bearing Immunosuppressive Exosomes in Leukemia/Lymphoma T and B Cells.
    Authors: Hedlund M, Nagaeva O, Kargl D, Baranov V, Mincheva-Nilsson L
    PLoS ONE, 2011-02-25;6(2):e16899.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  20. New insights into the phenotype of human dendritic cell populations.
    Authors: Clark GJ et al.
    Clin Transl Immunology

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