Human UNC5H3 Antibody Summary
Ala40-Asp376
Accession # O95185
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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UNC5H3 in Normal Human Kidney. UNC5H3 was detected in immersion fixed paraffin-embedded sections of normal human kidney (positive staining) and kidney cancer tissue (negative staining) using Mouse Anti-Human UNC5H3 Monoclonal Antibody (Catalog # MAB1005) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: UNC5H3
Caenorhabditis elegans UNC5 (UNC = behaviorally uncoordinated) and its mammalian homologues (including rat UNC5H1 and H2, mouse UNC5H2 and H3 (also known as rostral cerebellar malformation, RCM), and human UNC5H3 and H4) are transmembrane proteins belonging to the immunoglobulin (Ig) superfamily. All UNC5 family members have two Ig and two thrombospondin type 1 domains in their extracellular regions, as well as a conserved ZU-5 domain, a DCC (Deleted in Colorectal Cancer)-binding domain (DB) and a C-terminal death domain (DD) in their cytoplasmic regions (1, 2). UNC5 family proteins are receptors for the Netrin/UNC6 (netr: Sanskrit for “one who guides”) family of secreted axon guidance cues that are Laminin-related proteins. Netrin family proteins can act as chemoattractants for some axons and as chemorepellents for others. Besides UNC5, Netrin family proteins also bind to the DCC family of type I transmembrane receptors that share sequence similarity with proteins of the NCAM family, and adenosine A2b receptor, a G protein-coupled seven-transmembrane receptor belonging to the adenosine receptor family (3, 4). In vitro, Netrin binding to DCC family receptors in the absence of UNC5 is associated with axon attraction. However, the DCC-mediated attraction to Netrin is converted to repulsion by binding of UNC5 to the DCC-Netrin complex. In vivo, the mechanisms of Netrin-dependent axon attraction and repulsion are more complex and may include UNC5-mediated repulsion that is independent of DCC (1, 5). Besides their roles in axon guidance and neuronal migration, the UNC5 and DCC families also act as dependence receptors and exert pro-apoptotic effects in the absence of Netrin (6). Human UNC5H3 cDNA encodes a 931 amino acid (aa) type I membrane protein with a putative 39 aa signal peptide and 337 aa extracellular domain. The extracellular domain of human UNC5H3 shares approximately 98%, 73%, and 66% aa sequence similarity with mouse UNC5H3, rat UNC5H2, and human UNC5H4, respectively.
- Hong, K. et al. (1999) Cell 97:927.
- Leonardo, E.D. et al. (1997) Nature 386:833.
- Culotti, J.B. and D.C. Merz (1998) Curr. Opin. Cell Biol. 10:609.
- Corset, V. (2000) Nature 407:747.
- Merz, D.C. (2001) Genetics 158:1071.
- Llambi, F. et al. (2001) EMBO J. 20:2715.
Product Datasheets
Citations for Human UNC5H3 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Methylation of the UNC5C gene and its protein expression in colorectal cancer
Authors: J Wu, G Wang, B He, X Chen, Y An
Tumour Biol., 2017-04-01;39(4):1010428317697.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
A rare mutation in UNC5C predisposes to late-onset Alzheimer's disease and increases neuronal cell death
Authors: Monica K Wetzel-Smith, Julie Alzheimer's Disease Genetics Consortium, Tushar R Hunkapiller, Tushar R Srinivasan, Karpagam Srinivasan, Jasvinder K Maloney et al.
Nature Medicine
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Variants in the Netrin-1 Receptor UNC5C Prevent Apoptosis and Increase Risk for Familial Colorectal Cancer
Authors: Marie–May Coissieux, Jerneja Tomsic, Marie Castets, Heather Hampel, Sari Tuupanen, Nadine Andrieu et al.
Gastroenterology
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