Human Uromodulin Antibody Summary
Asp25-Ser614
Accession # P07911
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Uromodulin by Western Blot. Western blot shows lysates of human kidney tissue. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Uromodulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5144) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Uromodulin at approximately 80-90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Human Uromodulin by Simple WesternTM. Simple Western shows lysates of Exosome Standards (Human Urine) (NBP2-49840) and human kidney tissue, loaded at 0.5 mg/ml. A specific band was detected for Uromodulin at approximately 149 kDa (as indicated) using 50 µg/mL of Sheep Anti-Human Uromodulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5144). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.
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Uromodulin in Human Kidney. Uromodulin was detected in immersion fixed paraffin-embedded sections of human kidney using 1.7 µg/mL Sheep Anti-Human Uromodulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5144) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific labeling was localized to epithelial cells in tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Uromodulin
Uromodulin (also Tamm-Horsfall glycoprotein or THP) is an 85‑95 kDa urinary glycoprotein. It is secreted by renal tubule epithelium, acts as a binding protein for IL‑1, TNF-alpha and C1q, activates resting monocytes and promotes neutrophil phagocytosis. Uromodulin forms high molecular weight oligomers that line the kidnay tubules. Human Uromodulin is GPI‑linked. Its proprecursor is 616 amino acids (aa) in length. It contains three EGF-like domains (aa 28‑149), a ZP domain that mediates oligomerization (aa 334‑589) and a cleavable C‑terminal propeptide (aa 615‑640). There are multiple splice variants. One shows a deletion of aa 67‑199, a second shows a nine aa substitution for aa 609‑640, a third shows a Pro substitution for aa 205‑234 and a fourth shows a 66 aa substitution for aa 613‑640. Over aa 25‑614, human Uromodulin is 78% aa identical to mouse Uromodulin.
Product Datasheets
Citations for Human Uromodulin Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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In situ classification of cell types in human kidney tissue using 3D nuclear staining
Authors: Woloshuk A, Khochare S, Almulhim A et al.
Cytometry A
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Fine tuning the extracellular environment accelerates the derivation of kidney organoids from human pluripotent stem cells
Authors: Garreta E, Prado P, Tarantino C et al.
Nature Materials
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Fine tuning the extracellular environment accelerates the derivation of kidney organoids from human pluripotent stem cells
Authors: Garreta E, Prado P, Tarantino C et al.
Nature Materials
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Conserved and Divergent Molecular and Anatomic Features of Human and Mouse Nephron Patterning
Authors: Nils O. Lindström, Tracy Tran, Jinjin Guo, Elisabeth Rutledge, Riana K. Parvez, Matthew E. Thornton et al.
Journal of the American Society of Nephrology
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Tamm-Horsfall Protein Regulates Mononuclear Phagocytes in the Kidney
Authors: Radmila Micanovic, Shehnaz Khan, Danielle Janosevic, Maya E. Lee, Takashi Hato, Edward F. Srour et al.
Journal of the American Society of Nephrology
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