Uteroglobin is small, secreted protein of the secretoglobin (SCGBs) family. Uteroglobin is expressed by Clara cells, the non-ciliated, non-mucous secretory cells predominant in lung bronchioles, and by other epithelia that communicate with the external environment. Uteroglob in binds to phospholipids and to several cell types, inhibiting inflammation, migration and invasion. It has been reported to bind the lipocalin-1 receptor.
Human Uteroglobin Quantikine ELISA Kit
R&D Systems | Catalog # DUGB00
Loading...
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL), Saliva (50 µL), Urine (50 µL), Human Milk (50 µL)
Sensitivity
0.217 ng/mL
Assay Range
0.78-50 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk)
Loading...
Product Summary for Human Uteroglobin Quantikine ELISA Kit
The Quantikine Human Uteroglobin Immunoassay is a 4.5 hour solid phase ELISA designed to measure human Uteroglobin in cell culture supernates, serum, plasma, saliva, urine, and human milk. It contains E. coli-expressed recombinant human Uteroglobin and has been shown to accurately quantitate the recombinant factor. Results obtained measuring natural human Uteroglobin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Uteroglobin.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Species
Human
Specificity
Natural and recombinant human Uteroglobin
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.
精度
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 5.2 | 15.8 | 31.7 | 5.0 | 14.7 | 28.8 |
| Standard Deviation | 0.21 | 0.49 | 1.16 | 0.28 | 0.92 | 2.09 |
| CV% | 4.0 | 3.1 | 3.7 | 5.6 | 6.3 | 7.3 |
Human Milk, Saliva, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 5.1 | 15.4 | 31.3 | 5.2 | 15.4 | 29.9 |
| Standard Deviation | 0.29 | 0.34 | 1.04 | 0.31 | 0.72 | 2.25 |
| CV% | 5.7 | 2.2 | 3.3 | 6.0 | 4.7 | 7.5 |
Recovery for Human Uteroglobin Quantikine ELISA Kit
The recovery of Uteroglobin spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 102 | 99-109 |
| EDTA Plasma (n=4) | 95 | 85-107 |
| Heparin Plasma (n=4) | 95 | 86-109 |
| Serum (n=4) | 95 | 85-104 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Uteroglobin were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human Uteroglobin Quantikine ELISA Kit
Human Uteroglobin/SCGB1A1 ELISA Saliva/Urine/Human Milk Standard Curve
Human Uteroglobin/SCGB1A1 ELISA Cellculture Supernate/Serum/Plasma Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Uteroglobin/SCGB1A1
Alternate Names
Blastokinin, CC10, CC16, CCPBP, CCSP, Clara, PCB-BP, SCGB1A1, UGB
Gene Symbol
SCGB1A1
Additional Uteroglobin/SCGB1A1 Products
Product Documents for Human Uteroglobin Quantikine ELISA Kit
Product Specific Notices for Human Uteroglobin Quantikine ELISA Kit
For research use only
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.Related Research Areas
Citations for Human Uteroglobin Quantikine ELISA Kit
Powered by Bioz
Customer Reviews for Human Uteroglobin Quantikine ELISA Kit
There are currently no reviews for this product. Be the first to review Human Uteroglobin Quantikine ELISA Kit and earn rewards!
Have you used Human Uteroglobin Quantikine ELISA Kit?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
View specific protocols for Human Uteroglobin Quantikine ELISA Kit (DUGB00):
Refer to the product for complete assay procedure.
Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 100 µL of Assay Diluent to each well.
- Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
- Aspirate and wash 4 times.
- Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
- Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
100 µL Assay Diluent
50 µL Standard, Control, or Sample
200 µL Conjugate
200 µL Substrate Solution
50 µL Stop Solution
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- View all Protocols, Troubleshooting, Illustrated assays and Webinars