Human Uteroglobin/SCGB1A1 Antibody Summary
Glu22-Asn91
Accession # P11684
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Uteroglobin/SCGB1A1 in Human Lung. Uteroglobin/SCGB1A1 was detected in immersion fixed paraffin-embedded sections of human lung using 15 µg/mL Rat Anti-Human Uteroglobin/SCGB1A1 Monoclonal Antibody (Catalog # MAB4218) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of bronchiolar Clara cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Uteroglobin/SCGB1A1 in Human Lung. Uteroglobin/SCGB1A1 was detected in immersion fixed paraffin-embedded sections of human lung using Rat Anti-Human Uteroglobin/SCGB1A1 Monoclonal Antibody (Catalog # MAB4218) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Uteroglobin/SCGB1A1
Uteroglobin, also called Clara cell secretory, phospholipid binding, 10 kDa or 16 kDa protein (CCSP, CCPBP, CC10 or CC16, respectively) is a small, non‑glycosylated secreted protein of the secretoglobin superfamily, (designated 1A, member 1) (1‑3). Its name is derived from its very high expression in the pre‑implantation uterus. It is produced by the non-ciliated, non-mucous secretory cells that predominate in lung bronchioles (Clara cells), and other non-ciliated epithelia that communicate with the external environment (1‑3). Expression is induced by steroid hormones such as estrogen, and enhanced by the non-steroid hormone prolactin (1). Uteroglobin is found in blood, urine and other body fluids (1). Human Uteroglobin cDNA encodes a 21 amino acid (aa) signal sequence and a 70 aa mature protein. It shares 53‑56% aa identity with mouse, rat, bovine, canine, equine or rabbit Uteroglobin, and is active in mice (4). The mature protein forms a disulfide-linked head-to-tail homodimer of 16 kDa (2, 5). This homodimer is thought to form a binding pocket that binds hydrophobic ligands such as phospholipids, progesterone and retinols (5). Sequestering of prostaglandins and leukotrienes is anti-inflammatory, while sequestering of carcinogens such as polychlorinated bisphenols is anti-tumorigenic (6‑8). Other immunoregulatory activities of Uteroglobin include cell migration inhibition (by binding the chemotaxis-related formyl peptide receptor FPR2 on dendritic cells), and the inhibition of T cell differentiation to a Th2 phenotype (9). A single nucleotide polymorphism of Uteroglobin, A38G, confers increased risk of asthma (10). Transglutaminase can crosslink Uteroglobin, either to itself or to other proteins such as the adhesion molecule fibronectin (3, 11). Binding of fibronectin to Uteroglobin in the kidney is thought to protect against nephropathy, while binding of the lipocalin-1 receptor has been reported to suppress cancer cell motility and invasion (12, 13).
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- Wang, S-Z. et al. (2003) J. Immunol. 171:1051.
- Umland, T.C. et al. (1994) Nat. Struct. Biol. 1:538.
- Mandal, A.K. et al. (2004) J. Exp. Med. 199:1317.
- Watson, T.M. et al. (2001) Am. J. Physiol. Lung Cell Mol. Physiol. 281:L1523.
- Yang, Y. et al. (2004) J. Biol. Chem. 279:29336.
- Ray, R. et al. (2006) FEBS Lett. 580:6022.
- Martin, A.C. et al. (2006) Am. J. Respir. Crit. Care Med. 173:617.
- Antico, G. et al. (2006) J. Cell Physiol. 207:553.
- Zheng, F. et al. (1999) Nat. Med. 5:1018.
- Zhang, Z. et al. (2006) Gene 369:66.
Product Datasheets
Citations for Human Uteroglobin/SCGB1A1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Smoking shifts human small airway epithelium club cells toward a lesser differentiated population
Authors: Rostami MR, LeBlanc MG, Strulovici-Barel Y et al.
NPJ genomic medicine
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Release of Notch activity coordinated by IL-1 beta signalling confers differentiation plasticity of airway progenitors via Fosl2 during alveolar regeneration
Authors: Jinwook Choi, Yu Jin Jang, Catherine Dabrowska, Elhadi Iich, Kelly V. Evans, Helen Hall et al.
Nature Cell Biology
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Development of a miniaturized 96-Transwell air-liquid interface human small airway epithelial model
Authors: T Bluhmki, S Bitzer, JA Gindele, E Schruf, T Kiechle, M Webster, J Schymeinsk, R Ries, F Gantner, D Bischoff, J Garnett, R Heilker
Sci Rep, 2020-08-03;10(1):13022.
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Cigarette Smoke Specifically Affects Small Airway Epithelial Cell Populations and Triggers the Expansion of Inflammatory and Squamous Differentiation Associated Basal Cells
Authors: Wohnhaas CT, Gindele JA, Kiechle T et al.
Int J Mol Sci
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VEGF receptor 2 (KDR) protects airways from mucus metaplasia through a Sox9-dependent pathway
Authors: Ming Jiang, Yinshan Fang, Yu Li, Huachao Huang, Zichen Wei, Xia Gao et al.
Developmental Cell
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Autophagy proteins are required for club cell structure and function in airways
Authors: Malvin NP, Kern JT, Liu TC et al.
Am. J. Physiol. Lung Cell Mol. Physiol.
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TNFalpha and IL-17 Alkalinize Airway Surface Liquid through CFTR and Pendrin
Authors: Rehman T, Thornell IM, Pezzulo AA et al.
Am. J. Physiol., Cell Physiol.
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Apical-Out Human Airway Organoids Modeling SARS-CoV-2 Infection
Authors: Man Chun Chiu, Shuxin Zhang, Cun Li, Xiaojuan Liu, Yifei Yu, Jingjing Huang et al.
Viruses
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Human distal airways contain a multipotent secretory cell that can regenerate alveoli
Authors: Basil MC, Cardenas-Diaz FL, Kathiriya JJ et al.
Nature
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SARS-CoV-2 variants reveal features critical for replication in primary human cells
Authors: Marie O. Pohl, Idoia Busnadiego, Verena Kufner, Irina Glas, Umut Karakus, Stefan Schmutz et al.
PLOS Biology
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JAK-STAT activation contributes to cytotoxic T cell-mediated basal cell death in human chronic lung allograft dysfunction
Authors: A Khatri, JL Todd, FL Kelly, A Nagler, Z Ji, V Jain, SG Gregory, KJ Weinhold, SM Palmer
JCI Insight, 2023-03-22;8(6):.
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The in vitro multilineage differentiation and maturation of lung and airway cells from human pluripotent stem cell-derived lung progenitors in 3D
Authors: AL Rodrigues, HY Liu, YW Chen, M Porotto, A Moscona, HW Snoeck
Nature Protocols, 2021-03-01;0(0):.
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Changes in the Proportion of Each Cell Type After hiPSC-Derived Airway Epithelia Transplantation
Authors: Kitano, M;Hayashi, Y;Ohnishi, H;Okuyama, H;Yoshimatsu, M;Mizuno, K;Kuwata, F;Tada, T;Kishimoto, Y;Morita, S;Omori, K;
Cell transplantation
Species: Xenograft
Sample Types: Whole Tissue
Applications: IHC -
A single-cell atlas of in vitro multiculture systems uncovers the in vivo lineage trajectory and cell state in the human lung
Authors: Lee, W;Lee, S;Yoon, JK;Lee, D;Kim, Y;Han, YB;Kim, R;Moon, S;Park, YJ;Park, K;Cha, B;Choi, J;Kim, J;Ha, NY;Kim, K;Cho, S;Cho, NH;Desai, TJ;Chung, JH;Lee, JH;Kim, JI;
Experimental & molecular medicine
Species: Rat
Sample Types: Organoid
Applications: Immunocytochemistry/ Immunofluorescence -
Human distal airways contain a multipotent secretory cell that can regenerate alveoli
Authors: Basil MC, Cardenas-Diaz FL, Kathiriya JJ et al.
Nature
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Human distal lung maps and lineage hierarchies reveal a bipotent progenitor
Authors: P Kadur Laks, V Sontake, A Tata, Y Kobayashi, L Macadlo, K Okuda, AS Conchola, S Nakano, S Gregory, LA Miller, JR Spence, JF Engelhardt, RC Boucher, JR Rock, SH Randell, PR Tata
Nature, 2022-03-30;604(7904):111-119.
Species: Human
Sample Types: Whole Cells, Whole Tissue
Applications: ICC, IHC -
A medium composition containing normal resting glucose that supports differentiation of primary human airway cells
Authors: R Morgan, C Manfredi, KF Easley, LD Watkins, WR Hunt, SL Goudy, EJ Sorscher, M Koval, SA Molina
Scientific Reports, 2022-01-27;12(1):1540.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Single-cell analysis of human primary prostate cancer reveals the heterogeneity of tumor-associated epithelial cell states
Authors: H Song, HNW Weinstein, P Allegakoen, MH Wadsworth, J Xie, H Yang, EA Castro, KL Lu, BA Stohr, FY Feng, PR Carroll, B Wang, MR Cooperberg, AK Shalek, FW Huang
Nature Communications, 2022-01-10;13(1):141.
Species: Human
Sample Types: Organoids
Applications: IHC -
Human alveolar type 2 epithelium transdifferentiates into metaplastic KRT5+ basal cells
Authors: JJ Kathiriya, C Wang, M Zhou, A Brumwell, M Cassandras, CJ Le Saux, M Cohen, KD Alysandrat, B Wang, P Wolters, M Matthay, DN Kotton, HA Chapman, T Peng
Nature Cell Biology, 2021-12-30;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Pulmonary fibrosis distal airway epithelia are dynamically and structurally dysfunctional
Authors: IT Stancil, JE Michalski, D Davis-Hall, HW Chu, JA Park, CM Magin, IV Yang, BJ Smith, E Dobrinskik, DA Schwartz
Nature Communications, 2021-07-27;12(1):4566.
Species: Human
Sample Types: Whole Cells
Applications: IHC -
Pharmacological targeting of host chaperones protects from pertussis toxin in vitro and in vivo
Authors: K Ernst, AK Mittler, V Winkelmann, C Kling, N Eberhardt, A Anastasia, M Sonnabend, R Lochbaum, J Wirsching, M Sakari, AT Pulliainen, C Skerry, NH Carbonetti, M Frick, H Barth
Scientific Reports, 2021-03-08;11(1):5429.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
MDA5 Governs the Innate Immune Response to SARS-CoV-2 in Lung Epithelial Cells
Authors: X Yin, L Riva, Y Pu, L Martin-San, J Kanamune, Y Yamamoto, K Sakai, S Gotoh, L Miorin, PD De Jesus, CC Yang, KM Herbert, S Yoh, JF Hultquist, A García-Sas, SK Chanda
Cell Reports, 2021-01-12;34(2):108628.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Three-Dimensional Human Alveolar Stem Cell Culture Models Reveal Infection Response to SARS-CoV-2
Authors: J Youk, T Kim, KV Evans, YI Jeong, Y Hur, SP Hong, JH Kim, K Yi, SY Kim, KJ Na, T Bleazard, HM Kim, M Fellows, KT Mahbubani, K Saeb-Parsy, SY Kim, YT Kim, GY Koh, BS Choi, YS Ju, JH Lee
Cell Stem Cell, 2020-10-21;27(6):905-919.e10.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Osteopontin Expression in Small Airway Epithelium in Copd is Dependent on Differentiation and Confined to Subsets of Cells
Authors: MN Ali, M Mori, TCJ Mertens, P Siddhuraj, JS Erjefält, P Önnerfjord, PS Hiemstra, A Egesten
Sci Rep, 2019-10-29;9(1):15566.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Long-Term Modeling of SARS-CoV-2 Infection of In Vitro Cultured Polarized Human Airway Epithelium
Authors: Siyuan Hao, Kang Ning, Cagla Aksu Kuz, Kai Vorhies, Ziying Yan, Jianming Qiu
mBio
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A Cellular Anatomy of the Normal Adult Human Prostate and Prostatic Urethra
Authors: Gervaise H. Henry, Alicia Malewska, Diya B. Joseph, Venkat S. Malladi, Jeon Lee, Jose Torrealba et al.
Cell Reports
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Olfactomedin 4 mediation of prostate stem/progenitor-like cell proliferation and differentiation via MYC
Authors: Li H, Chaitankar V, Zhu J et al.
Scientific reports
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Inflammation-induced upregulation of P2X4 expression augments mucin secretion in airway epithelia
Authors: Veronika E. Winkelmann, Kristin E. Thompson, Kathrin Neuland, Ana M. Jaramillo, Giorgio Fois, Hanna Schmidt et al.
American Journal of Physiology-Lung Cellular and Molecular Physiology
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Cell lysis. 1:500 Dilution, incubate at 4 °C overnight.
DAPI - blue
Magenta -KRT5
Yellow - SCGB1A1
Cells were fixed in 4% Paraformaldehyde and stained with Human Uteroglobin/SCGB1A1 Antibody at 20µg/ml overnight at 4°C. As secondary antibodies Goat-anti-Rat-488 and Alexa Fluor647-Phalloidin were used.
