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Human VAMP-8 Antibody

Catalog #: AF5354
6 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF5354
AF5354-SP
Detection of Human VAMP‑8 by Western Blot.
2 Images
Product Details
Citations (6)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human VAMP-8 Antibody Summary

Species Reactivity
Human
Specificity
Detects human VAMP-8 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human VAMP-8
Met1-Lys75
Accession # Q9BV40
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
1-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human VAMP‑8 antibody by Western Blot. View Larger

Detection of Human VAMP‑8 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human VAMP-8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5354) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for VAMP-8 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunohistochemistry VAMP-8 antibody in Human Pancreas by Immunohistochemistry (IHC-P). View Larger

VAMP‑8 in Human Pancreas. VAMP-8 was detected in immersion fixed paraffin-embedded sections of human pancreas using Goat Anti-Human VAMP-8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5354) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to exocrine cell cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VAMP-8

VAMP-8 (vesicle associated membrane protein 8; also endobrevin) is a 12 kDa member of the synaptobrevin family of proteins. It is a type IV transmembrane (TM) protein (i.e.- a type II TM protein whose C-terminus is predominately transmembrane) that is found in cells involved in vesicle release (platelets, mast cells and pancreatic acinar cells). It regulates exocytosis and decouples granule release from cytokine release. Human VAMP-8 is 100 amino acids (aa) in length. It contains a vSNARE coiled-coil homology region (aa 12-72) and a membrane-anchor domain (aa 76‑96). Over aa 1‑75, human VAMP-8 shares 93% aa identity with mouse
VAMP‑2.

Long Name
Vesicle-Associated Membrane Protein 8
Entrez Gene IDs
8673 (Human); 22320 (Mouse); 83730 (Rat)
Alternate Names
EDB; EDBVAMP-8; Endobrevin; VAMP8; VAMP-8; vesicle-associated membrane protein 8 (endobrevin); vesicle-associated membrane protein 8

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Citations for Human VAMP-8 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Shiga toxin stimulates clathrin-independent endocytosis of the VAMP2, VAMP3 and VAMP8 SNARE proteins
    Authors: Henri-François Renard, Maria Daniela Garcia-Castillo, Valérie Chambon, Christophe Lamaze, Ludger Johannes
    Journal of Cell Science
  2. APOL1 variants change C-terminal conformational dynamics and binding to SNARE protein VAMP8
    Authors: SM Madhavan, JF O'Toole, M Konieczkow, L Barisoni, DB Thomas, S Ganesan, LA Bruggeman, M Buck, JR Sedor
    JCI Insight, 2017-07-20;2(14):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  3. Munc13-4 functions as a Ca2+ sensor for homotypic secretory granule fusion to generate endosomal exocytic vacuoles.
    Authors: Sang Su Woo, Declan J James, Thomas F J Martin
    Molecular Biology of the Cell, 2017-01-18;0(0):1939-4586.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. SNAP23 Regulates Endothelial Exocytosis of von Willebrand Factor.
    Authors: Zhu Q, Yamakuchi M, Lowenstein C
    PLoS ONE, 2015-08-12;10(8):e0118737.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: IHC, Western Blot
  5. Syntaxin 11 Contributes to the Interferon-Inducible Restriction of Coxiella burnetii Intracellular Infection
    Authors: S Ganesan, NN Alvarez, S Steiner, KM Fowler, AK Corona, CR Roy
    MBio, 2023-02-02;0(0):e0354522.
  6. Quantitative Proteomics Analysis of Lytic KSHV Infection in Human Endothelial Cells Reveals Targets of Viral Immune Modulation
    Authors: Ildar Gabaev, James C. Williamson, Thomas W.M. Crozier, Thomas F. Schulz, Paul J. Lehner
    Cell Reports

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