Human VAP-B Antibody

Catalog #: MAB5855 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5855
MAB5855-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human VAP-B Antibody Summary

Species Reactivity

Human

Specificity

Detects human VAP-B in direct ELISAs. In direct ELISAs, approximately 50% cross-reactivity with recombinant rat VAP-B is observed and no cross-reactivity with recombinant human VAP-A is observed.

Source

Monoclonal Mouse IgG_2B Clone # 695791

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human VAP-B
Ala2-Pro132
Accession # O95292

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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VAP‑B in Human Brain. VAP-B was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Mouse Anti-Human VAP-B Monoclonal Antibody (Catalog # MAB5855) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VAP-B

Vesicle-associated membrane protein (VAMP)-associated protein B (VAP-B; also VAMP-B) is an ~30 kDa ubiquitously expressed type IV transmembrane protein belonging to the VAP family (1, 2). It is found in endoplasmic reticulum (ER), Golgi and other membranes as a homodimer or a heterodimer with VAP-A, probably associating through a GxxxG motif in the transmembrane regions (1, 2). Human VAP-B cDNA encodes 243 amino acids (aa) that include a 222 aa cytoplasmic domain and a 21 aa C-terminal membrane anchor. The cytoplasmic domain contains a mobile sperm protein (MSP) domain (aa 7-124) and a coiled-coil region (aa 159‑196). Human VAP-B shares 90%, 89%, 96%, 96% and 94% aa identity with mouse, rat, canine, bovine and porcine VAP-B, respectively. VAP-A and VAP-B MSP domains recruit FFAT (two phenylalanines in an acidic tract)-motif-containing proteins to the cytosolic surface of ER membranes (2-4). FFAT proteins mediate many of the effects of VAPs on regulation of membrane transport, phospholipid biosynthesis, microtubule organization, and the unfolded protein response (2, 3). VAPs also interact with some SNARE and viral proteins (2). A human polymorphism of VAP-B, P56S, is found in three familial motor neuron diseases, notably the amylotrophic lateral sclerosis variant ALS8 (2). It produces a non-functional protein that can dimerize with and inhibit function of normal VAP-B, leading formation of intracellular aggregates and increased ER-stress-induced death of motor neurons (5-7). It can also promote cleavage and secretion of soluble VAP-B, which can then function as a ligand for EPH receptors (8). A naturally occurring 99 aa isoform of VAP-B that diverges at aa 71 within the MSP domain is termed VAP-C (1, 9). It also appears to be a negative regulator of VAP-A and VAP-B (9). While VAP-B is used by hepatitis C virus (HCV) for its propagation, VAP-C inhibits HCV propagation (9).

References

Nishimura, Y. et al. (1999) Biochem. Biophys. Res. Commun. 254:21.
Lev, S. et al. (2008) Trends Cell Biol. 18:282.
Peretti, D. et al. (2008) Mol. Biol. Cell 19:3871.
Kaiser, S.E. et al. (2005) Structure 13:1035.
Prosser, D.C. et al. (2008) J. Cell Sci. 121:3052.
Gkogkas, C. et al. (2008) Hum. Mol. Genet. 17:1517.
Suzuki, H. et al. (2009) J. Neurochem. 108:973.
Tsuda, H. et al. (2008) Cell 133:963.
Kukihara, H. et al. (2009) J. Virol. 83:7959.

Long Name

VAMP [Vesicle-associated Membrane Protein]-associated Protein B and C

Entrez Gene IDs

9217 (Human); 56491 (Mouse); 60431 (Rat)

Alternate Names

ALS8; ALS8VAMP-B/VAMP-C; VAMP (vesicle-associated membrane protein)-associated protein B and C; VAMP-associated 33 kDa protein; VAMP-associated protein B/C; VAMP-B; VAMP-C; VAPB; VAP-B; VAP-B/VAP-C; VAPC; VAP-C; vesicle-associated membrane protein-associated protein B/C