Human VIGR/GPR126 MAb (Clone 1044430)

Catalog #: MAB10577 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB10577-100
MAB10577-SP

Detection of VIGR/GPR126 in HEK293 Human Cell Line Transfected with Human VIGR/GPR126 by Flow Cytometry.

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All VIGR/GPR126 Antibodies

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Human VIGR/GPR126 MAb (Clone 1044430) Summary

Species Reactivity

Human

Specificity

Detects human VIGR/GPR126 protein in direct ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 1044430

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human embryonic kidney cell HEK293-derived human VIGR/GPR126 protein
Cys38-Lys437
Accession # AAH75798.1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

HEK293 Human Cell Line Transfected with Human VIGR/GPR126

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of VIGR/GPR126 in HEK293 Human Cell Line Transfected with Human VIGR/GPR126 by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human VIGR/GPR126 (filled histogram) or irrelevant protein (open histogram) was stained with Mouse Anti-Human VIGR/GPR126 Monoclonal Antibody (Catalog # MAB10577) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Gate was set based on Mouse IgG2A Isotype Control (MAB003). Staining was performed using our Staining Membrane-associated Proteins protocol.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VIGR/GPR126

VIGR (Vascular Inducible G Protein-coupled Receptor), also known as ADGRG6, DREG, and GPR126, is a neuronal 7 TM pass (G protein)-coupled receptor (GPCR) involved in myelination and glial and Schwann cell development (1, 2). Human VIGR cDNA encodes a 1221 amino acid (aa) residue membrane protein with a 37 aa signal peptide, a 825 aa extracellular domain (ECD) with 27 potential N-linked glycosylation sites, seven transmembrane segments that span between aa 863 and aa 1113, and a 108 aa residue cytoplasmic domain. Within ECD human VIGR shares 83% aa sequence identity with mouse and rat VIGR. VIGR is essential for the development of diverse organs (1, 2). Type IV collagen, a major constituent of the basement membrane, binds to VIGR and activates its signaling function (3). This interaction stimulated the production of cAMP in rodent Schwann cells, which require VIGR activity to differentiate, and in human embryonic kidney (HEK293) cells expressing exogenous VIGR. Laminin-211 binds a novel laminin-binding domain in VIGR N-terminal fragment between aa 446 and 807 (4). VIGR-Laminin-211 interactions regulate terminal differentiation and myelination by ensuring appropriate levels of cAMP for a given stage of Schwann cell development (4).

References

Rughetti, A. et al. (2005) J. Immunol. 174:7764.
Engelstaedter, V. et al. (2012) BMC Cancer 12:600.
Taylor-Papadimitriou, J. et al. (1999) Biochim. Biophys. Acta 1455:301.
Geng, Y. et al. (2012) Front Oncol. 2:76.
Tanida, S. et al. (2013) J Biol Chem. 288:31842.
Beatson, R. et al. (2016) Nat Immunol. 17:1273.
Piyush, T. et al. (2017) Cell Death Differ. 24:1937.

Long Name

Vascular-inducible G Protein-coupled Receptor

Entrez Gene IDs

57211 (Human); 215798 (Mouse); 308376 (Rat)

Alternate Names

APG1; Developmentally regulated G-protein-coupled receptor; DREG; FLJ14937; G protein-coupled receptor 126; Gm222; GPR126; G-protein coupled receptor 126; HBV PreS1-transactivated protein 2; PS1TP2; Vascular inducible G protein-coupled receptor; vascular-inducible G protein-coupled receptor; VIGR; VIGRPS1TP2