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Human Vitamin D BP Quantikine ELISA Kit

Catalog #: DVDBP0
36 Citations Datasheet / COA / SDS
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Human Vitamin D BP ELISA Standard Curve
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Product Details
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Citations (36)
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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human Vitamin D BP Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Saliva (25 uL), Urine (50 uL), Human Milk (10 uL)
Sensitivity
3.74 ng/mL
Assay Range
15.6 - 250 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk)
Specificity
Natural human Vitamin D Binding Protein
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine® Human Vitamin D Binding Protein immunoassay is a 3.5 hour solid phase ELISA designed to measure Vitamin D Binding Protein in cell culture supernates, serum, plasma, saliva, urine, and human milk. It contains natural human Vitamin D Binding Protein Standard. This kit can be used to determine mass values for naturally occurring human Vitamin D Binding Protein.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (ng/mL) 33 128 180 52.9 105 164
Standard Deviation 1.88 7.42 11.2 2.7 6.3 12.1
CV% 5.7 5.8 6.2 5.1 6 7.4

Recovery

The recovery of human Vitamin D Binding Protein spiked to three different levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 104 93-112

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human Vitamin D Binding Protein were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Human Vitamin D BP ELISA Linearity
Human Vitamin D BP ELISA Linearity

Scientific Data

N/A Human Vitamin D BP ELISA Standard Curve View Larger

Human Vitamin D BP ELISA Standard Curve

N/A Human Vitamin D BP Standard Curve View Larger

Human Vitamin D BP Standard Curve

Product Datasheets

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Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Vitamin D BP

Vitamin D binding protein (VitD BP), also known as DBP and Gc-globulin, is a 58 kDa glycoprotein that circulates at high concentration in the serum and serves as a carrier protein for vitamin D. The transport of vitamin D by VitD BP is important for the function of a wide variety of tissues. VitD BP binds both the 25(OH) and the hormonally active 1,25(OH)2 forms of vitamin D. VitD BP is primarily expressed in hepatocytes and to a lesser extent in the kidney. It delivers vitamin D into cells by Megalin-mediated endocytosis. A selectively deglycosylated form of VitD BP known as macrophage activating factor (MAF) blocks the angiogenic effects of FGF basic, VEGF, and Angiopoietin 2. VitD BP enhances the chemotaxis of monocytes and neutrophils to the activated complement component C5a or C5a des Arg (a C-terminally processed form of C5a). The chemotactic cofactor property of VitD BP is eliminated by binding to 1,25(OH)2 vitamin D, but it is not altered by binding to 25(OH) vitamin D or actin.

Long Name:
Vitamin D Binding Protein
Entrez Gene IDs:
2638 (Human); 14473 (Mouse); 24384 (Rat)
Alternate Names:
DBP; DBP/GC; DBP-MAF; DBPVDBG; EC 6.3.1.5; Gc; gc-globulin; Gc-MAF; GRD3; group-specific component (vitamin D binding protein); Group-specific component; hDBP; VDB; VDBG; VDBP; Vitamin D BP; vitamin D-binding alpha-globulin; vitamin D-binding protein

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 1 hour on a horizontal orbital microplate shaker.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  7.   Aspirate and wash 4 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
    9. 50 µL Stop Solution
  9.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human Vitamin D BP Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

36 Citations: Showing 1 - 10
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  1. Correlation of Plasma 25(OH)D3 and Vitamin D Binding Protein Levels with COVID-19 Severity and Outcome in Hospitalized Patients
    Authors: W Alabdullat, A Almnaizel, A Alhijji, A Alshathri, A Albarrag, I Bindayel
    Nutrients, 2023-04-10;15(8):.
    Species: Human
    Sample Types: Plasma
  2. Unravelling the Contribution of the rs7041 and rs4588 Polymorphisms of the GC Gene and Serum VDBP Levels for Developing Metabolic Syndrome in the Mexican Population
    Authors: A Hidalgo-Br, B Rivera-Par, G León-Reyes, N Patiño, M Castillejo, J Salmerón, R Velázquez-
    International Journal of Molecular Sciences, 2022-09-13;23(18):.
    Species: Human
    Sample Types: Serum
  3. Interplay between Maternal and Neonatal Vitamin D Deficiency and Vitamin-D-Related Gene Polymorphism with Neonatal Birth Anthropometry
    Authors: SS Lee, KH Ling, M Tusimin, R Subramania, KF Rahim, SP Loh
    Nutrients, 2022-01-27;14(3):.
    Species: Human
    Sample Types: Plasma
  4. Vitamin-D Binding Protein Gene Polymorphisms and Serum 25-Hydroxyvitamin-D in a Turkish Population
    Authors: L Karc?o?lu, A Özayd?n, ME Mavi?, GG Gürsu, L Harbige, N Hekim
    Metabolites, 2021-10-12;11(10):.
    Species: Human
    Sample Types: Serum
  5. Total, Bioavailable, and Free 25-Hydroxyvitamin D Equally Associate with Adiposity Markers and Metabolic Traits in Mexican Adults
    Authors: B Rivera-Par, A Hidalgo-Br, G León-Reyes, LS León-Maldo, A Aquino-Gál, M Castillejo, E Denova-Gut, YN Flores, J Salmerón, R Velázquez-
    Nutrients, 2021-09-23;13(10):.
    Species: Human
    Sample Types: Serum
  6. Correlational analysis of bone health status and vitamin D-related biomarkers in women working in agriculture
    Authors: MC Cho, KS Park, JK Shin, SA Lee, IA Cho, HC Jo, SC Kim, WJ Choi
    Medicine, 2021-08-27;100(34):e27071.
    Species: Human
    Sample Types: Serum
  7. Association of GC Variants with Bone Mineral Density and Serum VDBP Concentrations in Mexican Population
    Authors: B Rivera-Par, A Hidalgo-Br, G León-Reyes, B Antuna-Pue, YN Flores, J Salmerón, R Velázquez-
    Genes, 2021-07-29;12(8):.
    Species: Human
    Sample Types: Serum
  8. Longitudinal changes in vitamin D and its metabolites in pregnant South Africans
    Authors: JA George, SA Norris, T Snyman, L Khambule, M Toman, NJ Crowther
    The Journal of steroid biochemistry and molecular biology, 2021-07-07;212(0):105949.
    Species: Human
    Sample Types: Plasma
  9. Genome-wide association study of vitamin D concentrations and bone mineral density in the African American-Diabetes Heart Study
    Authors: ND Palmer, L Lu, TC Register, L Lenchik, JJ Carr, PJ Hicks, SC Smith, J Xu, L Dimitrov, J Keaton, M Guan, MCY Ng, YI Chen, AJ Hanley, CD Engelman, JM Norris, CD Langefeld, LE Wagenknech, DW Bowden, BI Freedman, J Divers
    PLoS ONE, 2021-05-20;16(5):e0251423.
    Species: Human
    Sample Types: Plasma
  10. Association of SNPs in GC and CYP2R1 with total and directly measured free 25-hydroxyvitamin D in multi-ethnic postmenopausal women in Saudi Arabia
    Authors: S Alharazy, MI Naseer, E Alissa, MD Robertson, S Lanham-New, MH Alqahtani, AG Chaudhary
    Saudi journal of biological sciences, 2021-05-04;28(8):4626-4632.
    Species: Human
    Sample Types: Serum
  11. Total vs. Bioavailable: Determining a Better 25(OH)D Index in Association with Bone Density and Muscle Mass in Postmenopausal Women
    Authors: NZ Abidin, SR Mitra
    Metabolites, 2020-12-31;11(1):.
    Species: Human
    Sample Types: Plasma
  12. Lack of association between Vitamin D status and free light chains profile with different chronic HCV-related liver and extrahepatic disorders
    Authors: U Basile, C Napodano, K Pocino, A Barini, M Marino, SA Santini, A Barini, A Stefanile, V Basile, CA Callà, P Cattani, A Gasbarrini, GL Rapaccini, F Gulli
    Eur Rev Med Pharmacol Sci, 2019-10-01;23(19):8506-8514.
    Species: Human
    Sample Types: Serum
  13. Metabolomics in plasma of Malawian children 7?years after surviving severe acute malnutrition: "ChroSAM" a cohort study
    Authors: C Bourdon, N Lelijveld, D Thompson, PS Dalvi, GB Gonzales, D Wang, M Alipour, E Wine, E Chimwezi, JC Wells, M Kerac, R Bandsma, MJ Nyirenda
    EBioMedicine, 2019-06-27;0(0):.
    Species: Human
    Sample Types: Plasma
  14. Free Vitamin D: Relationship to Insulin Sensitivity and Vascular Health in Youth
    Authors: F Bacha, SK Bartz, A Tomsa, S Sharma
    J. Pediatr., 2019-06-11;0(0):.
    Species: Human
    Sample Types: Plasma
  15. Differential proteome analysis in adolescent idiopathic scoliosis patients with thoracolumbar/lumbar curvatures
    Authors: H Makino, S Seki, I Kitajima, H Motomura, M Nogami, Y Yahara, N Ejiri, T Kimura
    BMC Musculoskelet Disord, 2019-05-24;20(1):247.
    Species: Human
    Sample Types: Plasma
  16. Cord Blood Vitamin D Levels and Early Childhood Blood Pressure: The Healthy Start Study
    Authors: KA Sauder, AV Stamatoiu, E Leshchinsk, BM Ringham, DH Glueck, D Dabelea
    J Am Heart Assoc, 2019-05-07;8(9):e011485.
    Species: Human
    Sample Types: Serum
  17. Prognostic significance of vitamin D receptor (VDR) gene polymorphisms in liver cirrhosis
    Authors: C Triantos, I Aggeletopo, M Kalafateli, PI Spantidea, G Vourli, G Diamantopo, D Tapratzi, M Michalaki, S Manolakopo, C Gogos, V Kyriazopou, A Mouzaki, K Thomopoulo
    Sci Rep, 2018-09-14;8(1):14065.
    Species: Human
    Sample Types: Serum
  18. Bipolar disorder in youth is associated with increased levels of vitamin D-binding protein
    Authors: B Petrov, A Aldoori, C James, K Yang, GP Algorta, A Lee, L Zhang, T Lin, RA Awadhi, JR Parquette, A Samogyi, LE Arnold, MA Fristad, B Gracious, O Ziouzenkov
    Transl Psychiatry, 2018-03-13;8(1):61.
    Species: Human
    Sample Types: Serum
  19. Clinical Utility of Measurement of Vitamin D-Binding Protein and Calculation of Bioavailable Vitamin D in Assessment of Vitamin D Status
    Authors: Yeo Min Yun
    Ann Lab Med, 2017-01-01;37(1):34-38.
    Species: Human
    Sample Types: Serum
  20. Bioavailable 25(OH)D but Not Total 25(OH)D Is an Independent Determinant for Bone Mineral Density in Chinese Postmenopausal Women
    Authors: C Li, P Chen, X Duan, J Wang, B Shu, X Li, Q Ba, J Li, Y Wang, H Wang
    EBioMedicine, 2016-11-28;15(0):184-192.
    Species: Human
    Sample Types: Plasma
  21. Genetically Low Vitamin D Levels, Bone Mineral Density, and Bone Metabolism Markers: a Mendelian Randomisation Study
    Sci Rep, 2016-09-14;6(0):33202.
    Species: Human
    Sample Types: Serum
  22. Pharmacokinetics and effects of demographic factors on blood 25(OH)D3 levels after a single orally administered high dose of vitamin D3
    Acta Pharmacol Sin, 2016-08-29;0(0):.
    Species: Human
    Sample Types: Plasma
  23. Urinary Vitamin D Binding Protein and KIM-1 Are Potent New Biomarkers of Major Adverse Renal Events in Patients Undergoing Coronary Angiography.
    Authors: Chaykovska L, Heunisch F, von Einem G, Alter M, Hocher C, Tsuprykov O, Dschietzig T, Kretschmer A, Hocher B
    PLoS ONE, 2016-01-11;11(1):e0145723.
    Species: Human
    Sample Types: Plasma
  24. Serum vitamin D levels are positively associated with varicella zoster immunity in chronic dialysis patients.
    Authors: Chao C, Lee S, Yang W, Yen C, Chiang C, Huang J, Hung K
    Sci Rep, 2014-12-09;4(0):7371.
    Species: Human
    Sample Types: Serum
  25. Associations between vascular health indices and serum total, free and bioavailable 25-hydroxyvitamin D in adolescents.
    Authors: Ashraf A, Alvarez J, Dudenbostel T, Calhoun D, Griffin R, Wang X, Hanks L, Gower B
    PLoS ONE, 2014-12-05;9(12):e114689.
    Species: Human
    Sample Types: Serum
  26. Serum vitamin D, vitamin D binding protein, and risk of colorectal cancer.
    Authors: Anic G, Weinstein S, Mondul A, Mannisto S, Albanes D
    PLoS ONE, 2014-07-18;9(7):e102966.
    Species: Human
    Sample Types: Serum
  27. Vitamin D-binding protein, circulating vitamin D and risk of renal cell carcinoma.
    Authors: Mondul A, Weinstein S, Moy K, Mannisto S, Albanes D
    Int J Cancer, 2014-01-30;134(11):2699-706.
    Species: Human
    Sample Types: Serum
  28. A bone substitute with high affinity for vitamin D-binding protein--relationship with niche of osteoclasts.
    Authors: Ikeda T, Kasai M, Tatsukawa E, Kamitakahara M, Shibata Y, Yokoi T, Nemoto T, Ioku K
    J Cell Mol Med, 2013-11-28;18(1):170-80.
    Species: Human
    Sample Types: Serum
  29. Circulating 25-hydroxyvitamin D, vitamin D-binding protein and risk of prostate cancer.
    Authors: Weinstein S, Mondul A, Kopp W, Rager H, Virtamo J, Albanes D
    Int J Cancer, 2012-12-27;132(12):2940-7.
    Species: Human
    Sample Types: Serum
  30. Vitamin D in a northern Canadian first nation population: dietary intake, serum concentrations and functional gene polymorphisms.
    Authors: Larcombe L, Mookherjee N, Slater J, Slivinski C, Singer M, Whaley C, Denechezhe L, Matyas S, Turner-Brannen E, Nickerson P, Orr P
    PLoS ONE, 2012-11-21;7(11):e49872.
    Species: Human
    Sample Types: Serum
  31. Influence of vitamin D binding protein on the association between circulating vitamin D and risk of bladder cancer.
    Br. J. Cancer, 2012-09-18;107(9):1589-94.
    Species: Human
    Sample Types: Serum
  32. Bioavailable vitamin D is more tightly linked to mineral metabolism than total vitamin D in incident hemodialysis patients.
    Authors: Bhan I, Powe CE, Berg AH
    Kidney Int., 2012-03-07;82(1):84-9.
    Species: Human
    Sample Types: Serum
  33. Accuracy of 6 routine 25-hydroxyvitamin D assays: influence of vitamin D binding protein concentration.
    Authors: Heijboer AC, Blankenstein MA
    Clin. Chem., 2012-01-12;58(3):543-8.
    Species: Human
    Sample Types: Plasma
  34. Vitamin D supplementation during pregnancy: double-blind, randomized clinical trial of safety and effectiveness.
    Authors: Hollis BW, Johnson D, Hulsey TC, Ebeling M, Wagner CL
    J. Bone Miner. Res., 2011-10-01;26(10):2341-57.
    Species: Human
    Sample Types: Serum
  35. Oral cholecalciferol decreases albuminuria and urinary TGF-beta1 in patients with type 2 diabetic nephropathy on established renin-angiotensin-aldosterone system inhibition.
    Authors: Kim MJ, Frankel AH, Donaldson M, Darch SJ, Pusey CD, Hill PD, Mayr M, Tam FW
    Kidney Int., 2011-08-10;80(8):851-60.
    Species: Human
    Sample Types: Urine
  36. Differences in bone metabolism between singleton pregnancy and twin pregnancy.
    Authors: Nakayama S, Yasui T, Suto M, Sato M, Kaji T, Uemura H, Maeda K, Irahara M
    Bone, 2011-05-27;49(3):513-9.
    Species: Human
    Sample Types: Serum

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