Human VSIG4 Antibody

Catalog #: MAB46464 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB46464-100
MAB46464-SP

Siglec-7 protein binding to VSIG4-transfected Human Cell Line is Blocked by VSIG4 Antibody.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human VSIG4 Antibody Summary

Species Reactivity

Human

Specificity

Detects human VSIG4 in direct ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 528906

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived human VSIG4 protein
Arg20-Pro283
Accession # Q9Y279

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Blockade of Receptor-ligand Interaction

50 µg/mL

In a functional flow cytometry test, 50 μg/mL of Mouse Anti-Human VSIG4 Antibody(Catalog # MAB46464) will block the binding of Recombinant Human Siglec-7/Fc protein (Catalog # 1138-SL) to HEK293 human embryonic kidney cell line transfected with recombinant human VSIG4.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Blockade of Receptor-ligand Interaction

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Siglec-7 protein binding to VSIG4-transfected Human Cell Line is Blocked by VSIG4 Antibody. In a functional flow cytometry test, Recombinant Human Siglec-7/Fc protein (1138-SL) binds to HEK293 human embryonic kidney cell line transfected with recombinant human VSIG4 and eGFP. (A) Binding is completely blocked by 50 µg/mL of Mouse Anti-Human VSIG4 Monoclonal Antibody (Catalog # MAB46464) but not by (B) Mouse IgG2A Isotype Control (MAB003). Protein binding was detected with Mouse Anti-Human IgG Fc APC-conjugated Monoclonal Antibody (FAB110A). Staining was performed using our Staining Membrane-Associated Proteins protocol.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VSIG4

VSIG4 (Vset and immunoglobulin domain containing 4), also known as complement receptor immunoglobulin (CRIg) and Z39IG, is a 45 kDa, type I transmembrane protein of the B7 family within the Ig superfamily that is expressed only in tissue resident macrophages (1-4). The gene is located on the X chromosome (2). The human VSIG4 cDNA encodes 399 amino acids (aa) including a 19 aa signal sequence, a 264 aa extracellular domain (ECD) containing a V-type and a C2-type Ig domain, a 21 aa transmembrane domain and a 95 aa cytoplasmic domain (3). The human VSIG4 ECD shares 84% aa identity with canine VSIG4. Within the IgV domain, it shares 90%, 80% and 78% aa identity with bovine, mouse and rat VSIG4, respectively; these animals lack the C2-type domain. Splice isoforms of 321, 305, 272, 201 and 199 aa lack all or part of the cytoplasmic domain, the C2-type Ig domain and/or the transmembrane domain (5). VSIG4 is specifically expressed on macrophages in the thymic medulla, peritoneum, alveoli, synovia, adipose and heart, liver Kupffer cells, placental Hofbauer cells, and atherosclerotic foam cells (1-4, 6-9). It is absent on infiltrating macrophages (8). VSIG4 is a complement receptor that binds C3b and iC3b fragments, internalizes them to recycling endosomes, and is recycled to the cell surface (4, 6). It contributes significantly to innate immunity by binding and phagocytosis of complement opsonized invading pathogens (4, 8, 10). Binding of either native or recombinant soluble VSIG4 to C3b inhibits complement amplification through the alternative, but not classical, pathway (10, 11). VSIG4 is also a negative regulator of mouse and human T cell activation (2). Although VSIG4 engagement may activate NF kappa B and thus be proinflammatory in some cases, many of its activities are important in resolving, rather than initiating, inflammation (1, 2, 7, 10, 11). There is emerging evidence in human conditions that VSIG4 may be a valuable biomarker in infection and immunity, inflammatory conditions and cancer prognosis (12, 13, 14).

References

He, J.Q. et al. (2008) Mol. Immunol. 4041.
Vogt, L. et al. (2006) J. Clin. Invest. 116:2817.
Langnaese, K. et al. (2000) Biochim. Biophys. Acta 1492:522.
Helmy, K. et al. (2006) Cell 124:915.
Entrez protein Accession # EAX05393, NP_001093901, CAI42052, CAI4205, EAX05394.
Tanaka, M. et al. (2008) Clin. Exp. Immunol. 154:38.
Lee, M.Y. et al. (2006) J. Leukoc. Biol. 80:922.
Gorgani, N.N. et al. (2008) J. Immunol. 181:7902.
Walker, M.G. (2002) Biochim. Biophys. Acta 1574:387.
Wiesmann, C. et al. (2006) Nature 444:217.
Katschke, K.J. et al. (2007) J. Exp. Med. 204:1319.
Small, A.G. et al. (2016) Swiss Med Wkly. 5:146.
Roh J. et al. (2017) Oncotarget. 8:58122.
Kim K.H. et al. (2016) Autophagy. 12:1647.

Long Name

V-Set and Immunoglobulin Domain Containing 4

Entrez Gene IDs

11326 (Human); 278180 (Mouse); 312102 (Rat); 102144784 (Cynomolgus Monkey)

Alternate Names

CRIg; Ig superfamily protein; Protein Z39Ig; V-set and immunoglobulin domain containing 4; V-set and immunoglobulin domain-containing protein 4; VSIG4; Z39IG; Z39IGcomplement receptor of the immunoglobulin superfamily