Human VSIG8 PE-conjugated Antibody

Catalog #: FAB9418P Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB9418P-100

Detection of VSIG8 in HEK293 Human Cell Line Transfected with Human VSIG8 and eGFP by Flow Cytometry.

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Human VSIG8 PE-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human VSIG8 in direct ELISAs. Stains human VSIG8 transfectants but not irrelevant transfectants in flow cytometry.

Source

Monoclonal Mouse IgG₁ Clone # 961823

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human embryonic kidney cell line HEK293-derived transfected with human VSIG8
Met1-Gly263
Accession # Q5VU13

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of VSIG8 in HEK293 Human Cell Line Transfected with Human VSIG8 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human VSIG8 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human VSIG8 PE-conjugated Monoclonal Antibody (Catalog # FAB9418P). Quadrant markers were set based on control antibody staining (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: VSIG8

VSIG8 (V-set and immunoglobulin domain containing 8), also known as C1orf204, is an approximately 45 kDa type I transmembrane protein of the B7 family within the Ig superfamily. Mature human VSIG8 consists of a 242 amino acid (aa) extracellular domain (ECD) containing two V-type Ig-like domains, a 21 aa transmembrane domain, and a 130 aa cytoplasmic domain. Within the ECD, human VSIG8 shares 88% and 89% aa identity with mouse and rat VSIG8, respectively. Alternative splicing generates a long isoform of human VSIG8 with a substitution in the cytoplasmic juxtamembrane region and a 124 aa extension at the C-terminus. VSIG8 was identified from proteomic analysis of human hair shafts (1, 2). It is expressed in the hair follicle and shaft, superficial layers of the nail matrix, and superficial layers of oral epithelium (3). R&D Systems in-house testing indicates that VSIG8 inhibits the production of IL-2 by activated T cells.

References

Rice, R.H. et al. (2010) J. Proteome Res. 9:6752.

Lee, Y.J. et al. (2006) Mol. Cell. Proteomics 5:789.

Rice, R.H. et al. (2011) J. Invest. Dermatol. 131:1936.

Long Name

V-Set and Immunoglobulin Domain Containing 8

Entrez Gene IDs

391123 (Human); 240916 (Mouse); 289236 (Rat); 102123183 (Cynomolgus Monkey)

Alternate Names

V-set and immunoglobulin domain containing 8; VSIG8