Human Xg Antibody

Catalog #: AF3699 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3699
AF3699-SP

Detection of Xg in Human Erythrocytes by Flow Cytometry.

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Human Xg Antibody Summary

Species Reactivity

Human

Specificity

Detects human Xg in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Xg
Gln22-Lys142
Accession # P55808

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Xg

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Xg in Human Erythrocytes by Flow Cytometry. Human blood erythrocytes were stained with Goat Anti-Human Xg Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3699, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Xg

Xg, also known as PBDX (pseudoautosomal boundary divided on the X chromosome), is a type I transmembrane glycoprotein that belongs to the CD99 family. It is an erythrocyte membrane protein that is used as the basis for the Xg blood group system. Mature Xg is a 26 kDa glycoprotein that consists of a 121 aa extracellular domain, a transmembrane segment, and a 17 aa cytoplasmic tail. Xg isoforms with either a 15 aa insertion or a deletion of nearly the entire ECD have been reported. Within the extracellular domain, human Xg shares 100% aa sequence identity with mouse Xg.

Long Name

Xg Blood Group

Entrez Gene IDs

7499 (Human)

Alternate Names

glycoprotein Xg; MGC118758; MGC118759; MGC118760; MGC118761; PBDX; Protein PBDX; Xg blood group (pseudoautosomal boundary-divided on the X chromosome); Xg blood group; XG glycoprotein; Xg; XGPY