Human ZNF143 Antibody

Name: Human ZNF143 Antibody
Brand: R&D Systems
SKU: MAB6804
Rating: 5 (1 reviews)

Catalog #: MAB6804 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6804
MAB6804-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ZNF143 Antibody Summary

Species Reactivity

Human

Specificity

Detects human ZNF143 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) ZNF24, rhZNF206, rhZNF281, rhDC-SCRIPT/ZNF366, rhZNF423, or recombinant mouse Bcl-6 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 677314

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human ZNF143
Gly459-Asp638
Accession # P52747

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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ZNF143 in BG01V Human Stem Cells. ZNF143 was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human ZNF143 Monoclonal Antibody (Catalog # MAB6804) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ZNF143

ZNF143 is a 69 kDa (unglycosylated) member of the GLI C2H2-type zinc-finger protein family. Human ZNF143 is 638 amino acids (aa) in length. A second isoform is produced from the deletion of residues 39-69. The protein contains seven C2H2-type zinc fingers, and is expressed in all tissues tested with greatest expression in the ovary. Functionally, ZNF143 serves as a transcriptional activator. Its other functions include acting as an activator for the gene for selenocysteine tRNA (tRNAsec), binding to the SPH motif of small nuclear RNA (snRNA) gene promoters, and participating in efficient U6 RNA polymerase III transcription through its interaction with CHD8. Human ZNF143 shares 97% aa sequence identity with mouse ZNF143.

Long Name

Zinc Finger Protein 143

Entrez Gene IDs

7702 (Human); 20841 (Mouse); 361627 (Rat)

Alternate Names

hStaf; pHZ-1; SBF; SBFzinc finger protein 143 (clone pHZ-1); Selenocysteine tRNA gene transcription-activating factor; SPH-binding factor; STAF; STAFpHZ-1; transcriptional activator Staf; zinc finger protein 143; ZNF143