Indocyanine green
Chemical Name: Sodium 4-(2-((1E,3E,5E,7Z)-7-(1,1-Dimethyl-3-(4-sulfonatobutyl)-1,3-dihydro-2H-benzo[e]indol-2-ylidene)hepta-1,3,5-trien-1-yl)-1,1-dimethyl-1H-benzo[e]indol-3-ium-3-yl)butane-1-sulfonate
Purity: ≥95%
Biological Activity
Key information: Indocyanine green (ICG) is a near-infrared (NIR) fluorescent dye suitable for in vivo imaging.Application: Fluorescence microscopy, in vivo imaging.
Properties and Photophysical Data: Indocyanine green is a NIR fluorescent dye operating in the 700-900 nm wavelength range. It binds to β-lipoproteins, particularly albumin, in plasma and exhibits fluorescence emission in the shortwave infrared window (SWIR; 1,000-2,000 nm) which enables in vivo imaging, including intravital microscopy, non-invasive real-time imaging in blood and lymph vessels, and imaging of hepatobiliary clearance. Imaging of indocyanine green in SWIR shows increased sensitivity, contrast, and resolution of fine anatomical structures over NIR. Excitation and emission maxima (λ) are 787 nm and 815 nm, respectively; quantum yield = 0.14; extinction coefficient = 223,000 M-1cm-1; A280 correction factor = 0.07.
This product is supplied with a high degree of hydration and may contain residual inorganic salts, the amount of which are batch dependent. Please refer to the Certificate of Analysis to obtain the batch specific Net Product Content.
Optical Data for Indocyanine green
| Emission Color | Near-IR |
|---|---|
| Brightness | |
| λabs | 787 nm |
| λem | 815 nm |
| Extinction Coefficient (ε) | 223000 M-1cm-1 |
| Quantum Yield (φ) | 0.14 |
| Closest Laser line | 785 nm |
| pH Sensitivity | No |
| Correction Factor 280 | 0.07 |
| Application | Fluorescence Microscopy, in vivo Imaging |
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Technical Data
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
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