Lactacystin

Tocris Bioscience | Catalog # 2267

Cell-permeable, potent and selective proteasome inhibitor
Tocris Bioscience

Key Product Details

Description

Cell-permeable, potent and selective proteasome inhibitor
View all Proteasome Inhibitors »

Product Description

Lactacystin is a cell-permeable, potent and selective proteasome inhibitor. A Streptomyces metabolite that is thought to bind irreversibly to the active site N-terminal threonine residue of the catalytic β-subunit of the 20S proteasome, thereby inhibiting its chymotrypsin and trypsin-like activities. Induces neurite outgrowth in Neuro 2a neuroblastoma cells and has been reported to induce apoptosis in human monoblast U937 cells. Also inhibits NF-κB activation (IC50 = 10 μM).

Product Specifications for Lactacystin

Molecular Weight

376.42

Formula

C15H24N2O7S

Storage

Store at -20°C

Purity

≥95% (HPLC)

Chemical Name

(2R,3S,4R)-3-Hydroxy-2-[(1S)-1-hydroxy-2-methylpropyl]-4-methyl-5-oxo-2-pyrrolidinecarboxy-N-acetyl-L-cysteine thioester

CAS Number

133343-34-7

PubChem ID

6610292

InChI Key

DAQAKHDKYAWHCG-RWTHQLGUSA-N

SMILES

O=C1N[C@]([C@@](SC[C@H](NC(C)=O)[C@](O)=O)=O)([C@H](C(C)C)O)[C@@H](O)[C@H]1C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
water 3.76 10

Preparing Stock Solutions for Lactacystin

The following data is based on the product molecular weight 376.42.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 26.57 mL 132.83 mL 265.66 mL
0.5 mM 5.31 mL 26.57 mL 53.13 mL
1 mM 2.66 mL 13.28 mL 26.57 mL
5 mM 0.53 mL 2.66 mL 5.31 mL

Calculators

Calculate the mass, volume, or concentration required for a solution.

=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Calculate the dilution required to prepare a stock solution.

x
=
x

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background References

References are publications that support the biological activity of the product. See our Citations tab to view 45 publications citing the usage of this product.

Product Documents for Lactacystin

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Lactacystin

For research use only

Related Research Areas

Notch Pathway

Customer Reviews for Lactacystin (1)

4 out of 5
1 Customer Rating
5 Stars
0%
4 Stars
100%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Lactacystin?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 的 1 review Showing All
Filter By:
Clear Filters X
  • lactacystin (10 M, 4 h)
    Name: Anonymous
    Species: Rat
    Assay Type: In Vitro
    Cell Line/Tissue: rat hippocampal neuronal
    Verified Customer | Posted 12/23/2019
    To tested the effects of inhibiting the proteolytic activity of the 26S proteasome complex via bath application of MG132 (50 M, 4 h) or lactacystin (10 M, 4 h) versus DMSO vehicle control.
    Lactacystin 2267

There are no reviews that match your criteria.

Showing  1 - 1 的 1 review Showing All