LTB4 Parameter Assay Kit Summary
Product Summary
Precision
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 40 | 40 | 40 |
Mean (pg/mL) | 170 | 481 | 847 | 164 | 516 | 879 |
Standard Deviation | 10 | 19 | 34 | 14.1 | 36.6 | 73.6 |
CV% | 5.8 | 4 | 4 | 8.6 | 7.1 | 8.4 |
Recovery
The recovery of LTB4 spiked to levels throughout the range of the assay in various matrices was evaluated.
Sample Type | Average % Recovery | Range % |
---|---|---|
Cell Culture Media (n=4) | 95 | 86-114 |
EDTA Plasma (n=4) | 91 | 82-112 |
Heparin Plasma (n=4) | 98 | 82-115 |
Serum (n=4) | 98 | 82-118 |
Linearity
Scientific Data
Product Datasheets
Preparation and Storage
Background: Leukotriene B4/LTB4
Leukotriene B4 (LTB4) is a major product of arachidonic acid metabolism and is formed via the 5-lipoxygenase pathway. LTB4 stimulates leukocyte functions including lysosomal enzyme release, adhesion, and aggregation of polymorphonuclear leukocytes. LTB4 has been implicated as a potent mediator of inflammatory diseases and immunoregulation.
Citations for LTB4 Parameter Assay Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
51
Citations: Showing 1 - 10
Filter your results:
Filter by:
-
Removal of circulating mitochondrial N-formyl peptides via immobilized antibody therapy restores sepsis-induced neutrophil dysfunction
Authors: Kwon, WY;Jung, YS;Suh, GJ;Kim, SH;Lee, A;Kim, JY;Kim, H;Park, H;Shin, J;Kim, T;Kim, KS;Itagaki, K;Hauser, CJ;
Journal of leukocyte biology
Species: Human
Sample Types: Plasma
-
Curcuma longa L. extract exhibits anti-inflammatory and cytoprotective functions in the articular cartilage of monoiodoacetate-injected rats
Authors: Kim, H;Jung, J;Lee, M;Kim, M;Kang, N;Kim, OK;Lee, J;
Food & nutrition research
Species: Rat
Sample Types: Tissue Homogenates
-
Evaluating the Anti-Osteoarthritis Potential of Standardized Boswellia serrata Gum Resin Extract in Alleviating Knee Joint Pathology and Inflammation in Osteoarthritis-Induced Models
Authors: Choi, YJ;Jung, JI;Bae, J;Lee, JK;Kim, EJ;
International journal of molecular sciences
Species: Rat
Sample Types: Serum
-
5-aminosalicylic acid suppresses osteoarthritis through the OSCAR-PPAR? axis
Authors: Kim, J;Ryu, G;Seo, J;Go, M;Kim, G;Yi, S;Kim, S;Lee, H;Lee, JY;Kim, HS;Park, MC;Shin, DH;Shim, H;Kim, W;Lee, SY;
Nature communications
Species: Mouse
Sample Types: Cell Culture Supernates
-
Disrupted balance between pro-inflammatory lipid mediators and anti-inflammatory specialized pro-resolving mediators is linked to hyperinflammation in patients with alcoholic hepatitis
Authors: Li, W;Xia, Y;Yang, J;Sanyal, AJ;Shah, VH;Chalasani, NP;Yu, Q;
medRxiv : the preprint server for health sciences
Species: Human
Sample Types: Plasma
-
Leukotriene A4 hydrolase inhibition improves age-related cognitive decline via modulation of synaptic function
Authors: Adams, JM;Rege, SV;Liu, AT;Vu, NV;Raina, S;Kirsher, DY;Nguyen, AL;Harish, R;Szoke, B;Leone, DP;Czirr, E;Braithwaite, S;Kerrisk Campbell, M;
Science advances
Species: Mouse
Sample Types: Plasma
-
TGF-?1 activates neutrophil signaling and gene expression but not migration
Authors: Hein, LE;SenGupta, S;Gunasekaran, G;Johnson, CN;Parent, CA;
PloS one
Species: Human
Sample Types: Cell Culture Supernates
-
TGF-?1 activates neutrophil signaling and gene expression but not migration
Authors: Hein, LE;SenGupta, S;Gunasekaran, G;Johnson, C;Parent, CA;
bioRxiv : the preprint server for biology
Species: Human
Sample Types: Cell Culture Supernates
-
Acute Coronary Syndrome May be Associated with Decreased Resolvin D1-to-Leukotriene B4 Ratio
Authors: C Sun, W Zheng, L Liang, Z Zhang, Z Liu, W Sun, J Zhou, W Qiao, Q Xie, R Tang
International heart journal, 2023-01-23;0(0):.
Species: Human
Sample Types: Plasma
-
Association of the Heart Rate Variability Response to Active Standing with the Severity of Calcific Aortic Valve Disease: Novel Insights of a Neurocardiovascular Pathology
Authors: J Rodríguez-, JM Torres-Are, N Ávila-Vanz, R Springall, R Bojalil, O Infante, C Lerma, JC Echeverría
Journal of Clinical Medicine, 2022-08-16;11(16):.
Species: Human
Sample Types: Serum
-
Spatiotemporal dynamics of calcium signals during neutrophil cluster formation
Authors: R Khazen, B Corre, Z Garcia, F Lemaître, S Bachellier, C d'Enfert, P Bousso
Oncogene, 2022-07-12;119(29):e2203855119.
Species: Mouse
Sample Types: Cell Culture Supernates
-
Substrate-dependent modulation of the leukotriene A4 hydrolase aminopeptidase activity and effect in a murine model of acute lung inflammation
Authors: KH Lee, NF Ali, SH Lee, Z Zhang, M Burdick, ZJ Beaulac, G Petruncio, L Li, J Xiang, EM Chung, KW Foreman, SM Noble, YM Shim, M Paige
Oncogene, 2022-06-08;12(1):9443.
Species: Mouse
Sample Types: BALF
-
Respiratory Syncytial Virus Nonstructural Protein 1 Promotes 5-Lipoxygenase via miR-19a-3p
Authors: M Zheng, P Fan, P Yang, J Zheng, D Zhao
Journal of Immunology Research, 2022-05-20;2022(0):4086710.
Species: Human
Sample Types: Cell Culture Supernates
-
Modeling of cancer-related body-wide effects identifies LTB4 as a diagnostic biomarker for pancreatic cancer
Authors: SH Jiang, D Liu, LP Hu, S Zhang, Y Yu, YW Sun, J Ji, ZG Zhang
EBioMedicine, 2022-05-10;80(0):104050.
Species: Human
Sample Types: Serum
-
Dietary rhamnogalacturonan-? rich extracts of molokhia ameliorate high fat diet-induced obesity and gut dysbiosis
Authors: HB Lee, MJ Oh, M Ho Do, Y Kim, I Choi, YS Kim, HY Park
The Journal of nutritional biochemistry, 2022-02-01;0(0):108954.
Species: Mouse
Sample Types: Serum
-
Danggui-Shaoyao-San improves cognitive impairment through inhibiting O-GlcNAc-modification of estrogen alpha receptor in female db/db mice
Authors: JJ Shi, HF Liu, T Hu, X Gao, YB Zhang, WR Li, Q Wang, SJ Zhang, D Tang, YB Chen
Journal of ethnopharmacology, 2021-08-23;281(0):114562.
Species: Mouse
Sample Types: Tissue Homogenates
-
Increased intestinal permeability with elevated peripheral blood endotoxin and inflammatory indices for e-waste lead exposure in children
Authors: X Luo, X Huo, Y Zhang, Z Cheng, S Chen, X Xu
Chemosphere, 2021-05-15;279(0):130862.
Species: Human
Sample Types: Serum
-
Chemotaxis and swarming in differentiated HL-60 neutrophil-like cells
Authors: KA Babatunde, X Wang, A Hopke, N Lannes, PY Mantel, D Irimia
Scientific Reports, 2021-01-12;11(1):778.
Species: Human
Sample Types: Cell Culture Supernates
-
S1PR4 ablation reduces tumor growth and improves chemotherapy via CD8+ T cell expansion
Authors: C Olesch, E Sirait-Fis, M Berkefeld, AF Fink, RM Susen, B Ritter, BE Michels, D Steinhilbe, FR Greten, R Savai, K Takeda, B Brüne, A Weigert
J. Clin. Invest., 2020-10-01;0(0):.
Species: Mouse
Sample Types: Cell Culture Supernates
-
TICAM2-related pathway mediates neutrophil exhaustion
Authors: R Lin, Y Zhang, K Pradhan, L Li
Sci Rep, 2020-09-01;10(1):14397.
Species: Mouse
Sample Types: Cell Culture Supernates
-
Minocycline microspheres did not significantly improve outcomes after collagenase injection of tendon
Authors: AD Allen, AM Bassil, DJ Berkoff, M Al Maliki, RW Draeger, PS Weinhold
J Orthop, 2019-06-03;16(6):580-584.
Species: Rat
Sample Types: Tissue Culture Supernates
-
Novel reprogramming of neutrophils modulates inflammation resolution during atherosclerosis
Authors: S Geng, Y Zhang, C Lee, L Li
Sci Adv, 2019-02-06;5(2):eaav2309.
Species: Mouse
Sample Types: Plasma
-
Serum leukotriene B4 levels, tonsillar hypertrophy and sleep-disordered breathing in childhood
Authors: E? Alexopoulo, G Haritos, C Befani, G Malakasiot, VA Lachanas, P Liakos, K Gourgoulia, AG Kaditis
Int. J. Pediatr. Otorhinolaryngol., 2018-08-07;113(0):218-222.
Species: Human
Sample Types: Serum
-
Early Production of the Neutrophil-Derived Lipid Mediators LTB4 and LXA4 Is Modulated by Intracellular Infection with Leishmania major
Authors: M Plagge, T Laskay
Biomed Res Int, 2017-10-18;2017(0):2014583.
Species: Human
Sample Types: Cell Culture Supernates
-
Suppressive Effect of the n-Hexane Extract of Litsea japonica Fruit Flesh on Monosodium-Iodoacetate-Induced Osteoarthritis in Rats
Authors: SH Kim, HJ Choi, WK Yang, JE Lee, JH Cho, IJ Park, S Park, BK Park, M Jin
Evid Based Complement Alternat Med, 2017-08-22;2017(0):1791403.
Species: Rat
Sample Types: Serum
-
Let-7 microRNA-dependent control of leukotriene signaling regulates the transition of hematopoietic niche in mice
Authors: X Jiang, JS Hawkins, J Lee, CO Lizama, FL Bos, JP Zape, P Ghatpande, Y Peng, J Louie, G Lagna, AC Zovein, A Hata
Nat Commun, 2017-07-25;8(1):128.
Species: Mouse
Sample Types: Cell Culture Supernates
-
Disrupting ceramide-CD300f interaction prevents septic peritonitis by stimulating neutrophil recruitment
Authors: K Izawa, A Maehara, M Isobe, Y Yasuda, M Urai, Y Hoshino, K Ueno, T Matsukawa, M Takahashi, A Kaitani, E Shiba, A Takamori, S Uchida, K Uchida, K Maeda, N Nakano, Y Yamanishi, T Oki, D Voehringer, A Roers, S Nakae, J Ishikawa, Y Kinjo, T Shimizu, H Ogawa, K Okumura, T Kitamura, J Kitaura
Sci Rep, 2017-06-27;7(1):4298.
Species: Mouse
Sample Types: Cell Culture Supernates
-
The development of novel LTA4H modulators to selectively target LTB4 generation
Authors: CM Low, S Akthar, DF Patel, S Löser, CT Wong, PL Jackson, JE Blalock, SA Hare, CM Lloyd, RJ Snelgrove
Sci Rep, 2017-03-17;7(0):44449.
Species: Human, Mouse
Sample Types: Cell Culture Supernates
-
Complement factor 5 blockade reduces porcine myocardial infarction size and improves immediate cardiac function
Authors: SE Pischke, A Gustavsen, HL Orrem, KH Egge, F Courivaud, H Fontenelle, A Despont, AK Bongoni, R Rieben, TI Tønnessen, MA Nunn, H Scott, H Skulstad, A Barratt-Du, TE Mollnes
Basic Res. Cardiol, 2017-03-03;112(3):20.
Species: Porcine
Sample Types: Plasma
-
Inflammatory Mediators in Tracheal Aspirates of Preterm Infants Participating in a Randomized Trial of Inhaled Nitric Oxide
Authors: M Laube, E Amann, U Uhlig, Y Yang, HW Fuchs, M Zemlin, JC Mercier, RF Maier, HD Hummler, S Uhlig, UH Thome
PLoS ONE, 2017-01-03;12(1):e0169352.
Species: Human
Sample Types: Tissue Secretion
-
The BLT1 Inhibitory Function of alpha-1 Antitrypsin Augmentation Therapy Disrupts Leukotriene B4 Neutrophil Signaling.
Authors: O'Dwyer C, O'Brien M, Wormald M, White M, Banville N, Hurley K, McCarthy C, McElvaney N, Reeves E
J Immunol, 2015-09-14;195(8):3628-41.
Species: Human
Sample Types: Plasma
-
Zileuton, 5-lipoxygenase inhibitor, acts as a chemopreventive agent in intestinal polyposis, by modulating polyp and systemic inflammation.
Authors: Gounaris E, Heiferman M, Heiferman J, Shrivastav M, Vitello D, Blatner N, Knab L, Phillips J, Cheon E, Grippo P, Khazaie K, Munshi H, Bentrem D
PLoS ONE, 2015-03-06;10(3):e0121402.
Species: Human, Mouse
Sample Types: Serum, Tissue Homogenates
-
The Src family kinases Hck, Fgr, and Lyn are critical for the generation of the in vivo inflammatory environment without a direct role in leukocyte recruitment.
Authors: Kovacs M, Nemeth T, Jakus Z, Sitaru C, Simon E, Futosi K, Botz B, Helyes Z, Lowell C, Mocsai A
J Exp Med, 2014-09-15;211(10):1993-2011.
Species: Mouse
Sample Types: Synovial Fluid
-
Endogenous omega-3 polyunsaturated fatty acid production confers resistance to obesity, dyslipidemia, and diabetes in mice.
Authors: Li J, Li F, Wei D, Jia W, Kang J, Stefanovic-Racic M, Dai Y, Zhao A
Mol Endocrinol, 2014-06-30;28(8):1316-28.
Species: Mouse
Sample Types: Serum
-
Lysosomal phospholipase A2: a novel player in host immunity to Mycobacterium tuberculosis.
Authors: Schneider B, Behrends J, Hagens K, Harmel N, Shayman J, Schaible U
Eur J Immunol, 2014-06-11;44(8):2394-404.
Species: Mouse
Sample Types: Tissue Homogenates
-
Role of leukotriene A4 hydrolase aminopeptidase in the pathogenesis of emphysema.
Authors: Paige M, Wang K, Burdick M, Park S, Cha J, Jeffery E, Sherman N, Shim Y
J Immunol, 2014-04-25;192(11):5059-68.
Species: Mouse
Sample Types: BALF
-
Leukotriene B4 levels in human atherosclerotic plaques and abdominal aortic aneurysms.
Authors: van den Borne P, van der Laan S, Bovens S, Koole D, Kowala M, Michael L, Schoneveld A, van de Weg S, Velema E, de Vries J, de Borst G, Moll F, de Kleijn D, Quax P, Hoefer I, Pasterkamp G
PLoS ONE, 2014-01-27;9(1):e86522.
Species: Human
Sample Types: Tissue Homogenates
-
Phosphoinositide 3-kinase inhibition restores neutrophil accuracy in the elderly: toward targeted treatments for immunosenescence.
Authors: Sapey E, Greenwood H, Walton G, Mann E, Love A, Aaronson N, Insall R, Stockley R, Lord J
Blood, 2013-11-04;123(2):239-48.
Species: Human
Sample Types: Sputum
-
Protective role of acetylsalicylic acid in experimental Trypanosoma cruzi infection: evidence of a 15-epi-lipoxin A(4)-mediated effect.
Authors: Molina-Berrios A, Campos-Estrada C, Henriquez N, Faundez M, Torres G, Castillo C, Escanilla S, Kemmerling U, Morello A, Lopez-Munoz R, Maya J
PLoS Negl Trop Dis, 2013-04-18;7(4):e2173.
Species: Mouse
Sample Types: Plasma
-
Usage of sphingosine kinase isoforms in mast cells is species and/or cell type determined.
Authors: Dillahunt S, Sargent J, Suzuki R, Proia R, Gilfillan A, Rivera J, Olivera A
J Immunol, 2013-01-28;190(5):2058-67.
Species: Mouse
Sample Types: Cell Culture Supernates
-
Circulating leukotriene B4 identifies respiratory complications after trauma.
Authors: Auner B, Geiger EV, Henrich D, Lehnert M, Marzi I, Relja B
Mediators Inflamm., 2012-03-07;2012(0):536156.
Species: Human
Sample Types: Plasma
-
Ornithodoros moubata complement inhibitor is an equally effective C5 inhibitor in pigs and humans.
Authors: Barratt-Due A, Thorgersen EB, Lindstad JK, Pharo A, Lissina O, Lambris JD, Nunn MA, Mollnes TE
J. Immunol., 2011-09-30;187(9):4913-9.
Species: Human, Porcine
Sample Types: Plasma
-
Abscisic acid regulates inflammation via ligand-binding domain-independent activation of peroxisome proliferator-activated receptor gamma.
Authors: Bassaganya-Riera J, Guri AJ, Lu P, Climent M, Carbo A, Sobral BW, Horne WT, Lewis SN, Bevan DR, Hontecillas R
J. Biol. Chem., 2010-11-18;286(4):2504-16.
Species: Mouse
Sample Types: Cell Culture Supernates
-
Lipid-cytokine-chemokine cascade drives neutrophil recruitment in a murine model of inflammatory arthritis.
Authors: Chou RC, Kim ND, Sadik CD
Immunity, 2010-08-27;33(2):266-78.
Species: Mouse
Sample Types: Cell Culture Supernates
-
MCP-1 antibody treatment enhances damage and impedes repair of the alveolar epithelium in influenza pneumonitis.
Authors: Narasaraju T, Ng HH, Phoon MC
Am. J. Respir. Cell Mol. Biol., 2009-07-17;42(6):732-43.
Species: Mouse
Sample Types: Serum
-
Participation of 5-lipoxygenase-derived LTB(4) in 4-hydroxynonenal-enhanced MMP-2 production in vascular smooth muscle cells.
Authors: Seo KW, Lee SJ, Kim CE
Atherosclerosis, 2009-06-18;208(1):56-61.
Species: Mouse
Sample Types: Cell Culture Supernates
-
Leukotriene B4 enhances the activity of nuclear factor-kappaB pathway through BLT1 and BLT2 receptors in atherosclerosis.
Authors: Sanchez-Galan E, Gomez-Hernandez A, Vidal C, Martin-Ventura JL, Blanco-Colio LM, Munoz-Garcia B, Ortega L, Egido J, Tuñón J
Cardiovasc. Res., 2008-10-13;81(1):216-25.
Species: Human
Sample Types: Plasma
-
Effects of atorvastatin added to inhaled corticosteroids on lung function and sputum cell counts in atopic asthma.
Authors: Hothersall EJ, Chaudhuri R, McSharry C, Donnelly I, Lafferty J, McMahon AD, Weir CJ, Meiklejohn J, Sattar N, McInnes I, Wood S, Thomson NC
Thorax, 2008-08-29;63(12):1070-5.
Species: Human
Sample Types: Sputum
-
CPAP decreases plasma levels of soluble tumour necrosis factor-alpha receptor 1 in obstructive sleep apnoea.
Authors: Arias MA, Garcia-Rio F, Alonso-Fernandez A, Hernanz A, Hidalgo R, Martinez-Mateo V, Bartolome S, Rodriguez-Padial L
Eur. Respir. J., 2008-05-28;32(4):1009-15.
Species: Human
Sample Types: Plasma
-
Effect of intensive atorvastatin therapy on prostaglandin E2 levels and metalloproteinase-9 activity in the plasma of patients with non-ST-elevation acute coronary syndrome.
Authors: Gomez-Hernandez A, Sanchez-Galan E, Ortego M, Martin-Ventura JL, Blanco-Colio LM, Tarin-Vicente N, Jimenez-Nacher JJ, Lopez-Bescos L, Egido J, Tuñón J
Am. J. Cardiol., 2008-04-22;102(1):12-8.
Species: Human
Sample Types: Plasma
-
Improved pulmonary function by acid sphingomyelinase inhibition in a newborn piglet lavage model.
Authors: von Bismarck P, Wistadt CF, Klemm K, Winoto-Morbach S, Uhlig U, Schutze S, Adam D, Lachmann B, Uhlig S, Krause MF
Am. J. Respir. Crit. Care Med., 2008-02-28;177(11):1233-41.
Species: Porcine
Sample Types: BALF
FAQs
No product specific FAQs exist for this product, however you may
View all ELISA FAQsReviews for LTB4 Parameter Assay Kit
Average Rating: 5 (Based on 1 Review)
Have you used LTB4 Parameter Assay Kit?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by: