When using MacCellect Cell Isolation kits (MAGM*** or MAGH***), why is it important to lyse red blood cells before starting the cell isolation protocol?

Name: MagCellect Mouse CD3+ T Cell Isolation Kit
Brand: R&D Systems
SKU: MAGM201
Price: 559 USD

It is important to remove red blood cells before the isolation protocol using MagCellect isolation kits because not doing so can cause a poorer yield of selected cells. Red blood cells become sticky during the incubation protocols and may interfere with the interactions needed for removal of unwanted populations.

MagCellect Mouse CD3+ T Cell Isolation Kit

Catalog #: MAGM201
11 Citations Datasheet / COA / SDS

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MAGM201

R&D Systems Magcellect Cell Isolation Kits

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Citations (11)

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MagCellect Mouse CD3+ T Cell Isolation Kit Summary

Specifications

Source

N/A

Shipping Conditions

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Species

Mouse

Product Datasheets

Product Datasheet

COA

SDS

Background: CD3

The T cell receptor (TCR)/CD3 complex consists of two variable antigen-recognition receptor chains (TCR-alpha/TCR-beta or TCR-gamma/TCR-delta) that are non-covalently linked to at least four different invariant chains: CD3 gamma, CD3 delta, CD3 epsilon, and the CD3 zeta chain that exists as either a homodimer or as a heterodimer with its splice-variant the CD3 eta chain. The CD3 complex functions to transduce intracellular signals during TCR antigen recognition. CD3 is expressed early in thymocyte development, and on a subset of NK cells. CD3 epsilon is one of at least three invariant proteins that associate with the variable antigen recognition chains of the T cell receptor and function in signal transduction.

Entrez Gene IDs

916 (Human); 12501 (Mouse)

Alternate Names

CD_antigen: CD3e; CD3 antigen, delta subunit; CD3; CD3d antigen; CD3d antigen, delta polypeptide (TiT3 complex); CD3d molecule, delta (CD3-TCR complex); CD3-DELTA; CD3e antigen; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3e; CD3-epsilon; CD3g antigen; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD3G; CD3-GAMMA; FLJ17620; FLJ17664; FLJ18683; FLJ79544; FLJ94613; IMD18; MGC138597; T3DOKT3, delta chain; T3E; T-cell antigen receptor complex, epsilon subunit of T3; T-cell receptor T3 delta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; TCRE

Related Research Areas

Citations for MagCellect Mouse CD3+ T Cell Isolation Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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Autoimmunity against melanoma differentiation-associated gene 5 induces interstitial lung disease mimicking dermatomyositis in mice
Authors: Ichimura, Y;Konishi, R;Shobo, M;Tanaka, R;Kubota, N;Kayama, H;Takeda, K;Nomura, T;Fujimoto, M;Okiyama, N;
Proceedings of the National Academy of Sciences of the United States of America 2024-04-16
Single-cell transcriptome profiling of the stepwise progression of head and neck cancer
Authors: JH Choi, BS Lee, JY Jang, YS Lee, HJ Kim, J Roh, YS Shin, HG Woo, CH Kim
Nature Communications, 2023-02-24;14(1):1055. 2023-02-24
Precision targeting of autoantigen-specific B cells in muscle-specific tyrosine kinase myasthenia gravis with chimeric autoantibody receptor T cells
Authors: S Oh, X Mao, S Manfredo-V, J Lee, D Patel, EJ Choi, A Alvarado, E Cottman-Th, D Maseda, PY Tsao, CT Ellebrecht, SL Khella, DP Richman, KC O'Connor, U Herzberg, GK Binder, MC Milone, S Basu, AS Payne
Nature Biotechnology, 2023-01-19;0(0):. 2023-01-19
Inhibiting ACK1-mediated phosphorylation of C-terminal Src kinase counteracts prostate cancer immune checkpoint blockade resistance
Authors: D Sridaran, S Chouhan, K Mahajan, A Renganatha, C Weimholt, S Bhagwat, M Reimers, EH Kim, MK Thakur, MA Saeed, RK Pachynski, MA Seeliger, WT Miller, FY Feng, NP Mahajan
Nature Communications, 2022-11-14;13(1):6929. 2022-11-14
Intrinsic Photosensitivity Enhances Motility of T Lymphocytes
Sci Rep, 2016-12-20;6(0):39479. 2016-12-20
Eriobotrya japonica Water Extract Characterization: An Inducer of Interferon-Gamma Production Mainly by the JAK-STAT Pathway
Authors: Khalid Z Matalka
Molecules, 2016-06-02;21(6):. 2016-06-02
Dichloroacetate modulates cytokines toward T helper 1 function via induction of the interleukin-12-interferon-gamma pathway.
Authors: Badr M, Qinna N, Qadan F, Matalka K
Onco Targets Ther, 2014-02-07;7(0):193-201. 2014-02-07
T-cell reconstitution during murine acquired immunodeficiency syndrome (MAIDS) produces neuroinflammation and mortality in animals harboring opportunistic viral brain infection.
Authors: Mutnal, Manohar, Schachtele, Scott J, Hu, Shuxian, Lokensgard, James R
J Neuroinflammation, 2013-07-31;10(1):98. 2013-07-31
hsBAFF regulates proliferation and response in cultured CD4(+) T lymphocytes by upregulation of intracellular free Ca(2+) homeostasis.
Authors: Wen L, Chen SJ, Zhang W, Ma HW, Zhang SQ, Chen L
Cytokine, 2010-12-08;53(2):215-22. 2010-12-08
Cytokine-dependent regulation of NADPH oxidase activity and the consequences for activated T cell homeostasis.
Authors: Purushothaman D, Sarin A
J. Exp. Med., 2009-06-22;206(7):1515-23. 2009-06-22

Evidence for a role for notch signaling in the cytokine-dependent survival of activated T cells.
Authors: Bheeshmachar G, Purushotaman D, Sade H, Gunasekharan V, Rangarajan A, Sarin A
J. Immunol., 2006-10-15;177(8):5041-50. 2006-10-15

FAQs

When using MacCellect Cell Isolation kits (MAGM*** or MAGH***), why is it important to lyse red blood cells before starting the cell isolation protocol?
- It is important to remove red blood cells before the isolation protocol using MagCellect isolation kits because not doing so can cause a poorer yield of selected cells. Red blood cells become sticky during the incubation protocols and may interfere with the interactions needed for removal of unwanted populations.

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