When using MacCellect Cell Isolation kits (MAGM*** or MAGH***), why is it important to lyse red blood cells before starting the cell isolation protocol?

Name: MagCellect Plus Human CD45+ Cell Isolation Kit
Brand: R&D Systems
SKU: MAGH125
Price: 749 USD

It is important to remove red blood cells before the isolation protocol using MagCellect isolation kits because not doing so can cause a poorer yield of selected cells. Red blood cells become sticky during the incubation protocols and may interfere with the interactions needed for removal of unwanted populations.

MagCellect Plus Human CD45+ Cell Isolation Kit

Catalog #: MAGH125 Datasheet / COA / SDS

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MAGH125

R&D Systems Magcellect Cell Isolation Kits

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MagCellect Plus Human CD45+ Cell Isolation Kit Summary

Specifications

Source

N/A

Shipping Conditions

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Species

Human

Product Datasheets

Product Datasheet

COA

SDS

Background: CD45

CD45 is a transmembrane protein tyrosine phosphatase. It is expressed at high levels on the cell surface of all nucleated hematopoietic cells and their precursors. Alternative splicing of CD45 mRNA results in the expression of several distinct isoforms that are differentially expressed in cells of different lineages and activation states.

Long Name

Cluster of Differentiation 45

Entrez Gene IDs

5788 (Human); 19264 (Mouse); 490255 (Canine); 100061950 (Equine)

Alternate Names

B220; CD_antigen: CD45; CD45 antigen; CD45; CD45R; EC 3.1.3.48; EC:3.1.3.48; GP180; LCA; L-CA; Leukocyte common antigen; LY5; protein tyrosine phosphatase, receptor type, c polypeptide; PTPRC; receptor-type tyrosine-protein phosphatase C; T200 Glycoprotein; T200 leukocyte common antigen; T200

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FAQs

When using MacCellect Cell Isolation kits (MAGM*** or MAGH***), why is it important to lyse red blood cells before starting the cell isolation protocol?
- It is important to remove red blood cells before the isolation protocol using MagCellect isolation kits because not doing so can cause a poorer yield of selected cells. Red blood cells become sticky during the incubation protocols and may interfere with the interactions needed for removal of unwanted populations.

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