Monocyte and Dendritic Cell Subsets Flow Cytometry Panel

Catalog #: FMC-P-008 Datasheet / COA / SDS

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FMC-P-008

Multicolor flow cytometry panel to identify monocyte and dendritic cell subsets.

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Monocyte and Dendritic Cell Subsets Flow Cytometry Panel Summary

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Specifications

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Species

Human

Product Datasheets

Product Datasheet

COA

SDS

Scientific Data

Flow Cytometry

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Multicolor flow cytometry panel to identify monocyte and dendritic cell subsets. . Monocyte and dendritic cell subsets were determined first based on expression of CD14 and HLA-DR. CD14+ monocytes were then gated into CD16- Classical Monocytes and CD16+ Non-Classical Monocytes. CD14-HLA-DR+ Dendritic Cells were then gated into CD11c+ CD123- monocyte-derived dendritic cells (mDCs) and CD11c-CD123+ plasmacytoid dendritic cells (pDCs). Cells were stained with Anti-Human HLA-DR Alexa Fluor® 405, CD8 FITC, CD11c PE, CD123 PerCP, CD16 APC, CD20 Alexa Fluor 700, CD3 Alexa Fluor 700, CD56 Alexa Fluor 700, and CD14 Alexa Fluor 750. Find Staining Protocol Here.