Mouse Activin RIB/ALK-4 Antibody

Catalog #: MAB1477 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1477
MAB1477-SP

Product Details

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Activin RIB/ALK-4 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Activin RIB/ALK-4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, shows 100% cross-reactivity with recombinant human (rh) Activin RIB, 20% cross-reactivity with rhActivin RIA, and no cross-reactivity with rhActivin RIIA or rhActivin RIIB.

Source

Monoclonal Rat IgG_2A Clone # 207304

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Activin RIB/ALK-4
Leu32-Glu126
Accession # NP_031421

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse Activin RIB/ALK‑4 Fc Chimera (Catalog # 1477-AR)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Activin RIB/ALK-4

Activin RIB, also known as ALK-4, is a glycosylated 58 kDa type I transmembrane receptor that belongs to the superfamily of TGF-beta serine/threonine kinase receptors. Activin RIB associates with Activin RIIB to form a receptor complex for activin and inhibin molecules (1). These ligands bind to Activin RIIB which then associates with, and phosphorylates, the cytoplasmic domain of Activin RIB to initiate signal transduction (2, 3). Mature mouse Activin RIB consists of a 103 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane segment, and a 356 aa cytoplasmic region that includes the kinase domain (4). Within the ECD, mouse Activin RIB shares 93% and 98% aa sequence identity with human and rat Activin RIB, respectively. It shares 23% - 38% aa sequence identity with other mouse type I receptors Activin RIA, BMPR-IA, BMPR-IB, and TGF-beta RI. Activin receptor signaling is modulated by the direct interaction of Activin RIB with cripto or inhibin binding protein (5 - 7). Activin RIB is excluded from the signaling complex if Activin RIIB first binds inhibin and betaglycan (8). Activin RIB functions in a wide variety of growth and differentiation processes, including embryonic cell fate and axis determination, cell proliferation, apoptosis, and tumorigenesis (1, 9, 10).

References

Chen, Y.-G. et al. (2006) Exp. Biol. Med. 231:534.
Attisano, L. et al. (1996) Mol. Cell. Biol. 16:1066.
Tsuchida, K. et al. (1995) Endocrinology 136:5493.
Ebner, R. et al. (1993) Science 260:1344.
Chapman, S.C. and T.K. Woodruff (2001) Mol. Endocrinol. 15:668.
Bianco, C. et al. (2002) Mol. Cell. Biol. 22:2586.
Gray, P.C. et al. (2003) Proc. Natl. Acad. Sci. 100:5193.
Lewis, K.A. et al. (2000) Nature 404:411.
Gu, Z. et al. (1998) Genes Dev. 12:844.
Chen, Y. et al. (2004) Dev. Biol. 268:280.

Long Name

Activin Receptor IB

Entrez Gene IDs

91 (Human); 11479 (Mouse)

Alternate Names

activin A receptor, type IB; Activin receptor type IB; Activin receptor-like kinase 4; Activin RIB; ActivinRIB; ACTRIB; ACVR1B; ACVRLK4ACTR-IB; ALK4; ALK-4; ALK4activin A receptor, type II-like kinase 4; EC 2.7.11; EC 2.7.11.30; Serine/threonine-protein kinase receptor R2; SKR2activin receptor type-1B