Mouse Alkaline Phosphatase/ALPL Antibody Summary
Phe18-Gly503
Accession # P09242
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
Detection of Mouse Alkaline Phosphatase/ALPL by Western Blot. Western blot shows lysates of D3 mouse embryonic stem cell line, MEF mouse embryonic feeder cells, and mouse embryo tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Alkaline Phosphatase/ALPL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2910) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for Alkaline Phosphatase/ALPL at approximately 75-80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of ALPL in Rat Stem Cells by Flow Cytometry. Rat Mesenchymal Stem Cells were stained with Goat Anti-Mouse Alkaline Phosphatase/ALPL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2910, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).
Alkaline Phosphatase/ALPL in Rat Mesenchymal Stem Cells. Alkaline Phosphatase/ALPL was detected in immersion fixed rat mesenchymal stem cells using Goat Anti-Mouse Alkaline Phosphatase/ALPL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2910) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Alkaline Phosphatase/ALPL in Mouse Embryo. Alkaline Phosphatase/ALPL was detected in immersion fixed frozen sections of mouse embryonic (E13.5) developing vertebra using Goat Anti-Mouse Alkaline Phosphatase/ALPL Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF2910) at 2 µg/mL overnight at 4 °C. Tissue was stained red and counterstained with DAPI (blue).Image courtesy of Paul J. Simmons, Ph.D., Director, Center for Stem Cell Research, The University of Texas Health Science Center at Houston, Houston, Texas, USA.View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Alkaline Phosphatase/ALPL
Several distinct genes encode alkaline phosphatases (APs) in mice with different tissue-specific expression patterns. The Alpl gene, also known as Akp2, encodes the liver/bone/kidney isozyme, also known as the tissue-nonspecific AP (TNAP) (1). The Alpl gene is a key regulator of bone mineralization in mice (2). A variety of mutations in the human ALPL gene leads to different forms of hypophosphatasia, characterized by poorly mineralized cartilage and bones (3). The native ALPL is a glycosylated homodimer attached to the membrane through a GPI-anchor. The C-terminal pro peptide (residues 504 to 524) is not present in the mature form.
- Terao, M. and B. Mintz (1987) Proc. Natl. Acad. Sci. USA 84:7051.
- Hessle, L. et al. (2002) Proc. Natl. Acad. Sci. USA 99:9445.
- Di Mauro, S. et al. (2002) J. Bone Miner. Res. 17:1383.
Product Datasheets
Citations for Mouse Alkaline Phosphatase/ALPL Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Genistein and menaquinone-4 treatment-induced alterations in the expression of mRNAs and their products are beneficial to osteoblastic MC3T3-E1 cell functions
Authors: M Katsuyama, M Demura, H Katsuyama, H Tanii, K Saijoh
Mol Med Rep, 2017-05-25;0(0):.
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Brain Endothelial Cells Are Exquisite Sensors of Age-Related Circulatory Cues
Authors: MB Chen, AC Yang, H Yousef, D Lee, W Chen, N Schaum, B Lehallier, SR Quake, T Wyss-Coray
Cell Rep, 2020-03-31;30(13):4418-4432.e4.
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Comparative Effects on Fetal Hematopoiesis and Placental Inflammation From Mesenchymal and Hematopoietic Stem Cells as Agents of Transamniotic Stem Cell Therapy (TRASCET) in a Syngeneic Model of Intrauterine Growth Restriction
Authors: Moskowitzova, K;Naus, AE;Kycia, I;Dang, TT;Shroff, YV;Bletsas, E;Mullin, K;Zurakowski, D;Fauza, DO;
Journal of pediatric surgery
Species: Rat
Sample Types: Whole Cells
Applications: Flow Cytometry -
The structural pathology for hypophosphatasia caused by malfunctional tissue non-specific alkaline phosphatase
Authors: Yu, Y;Rong, K;Yao, D;Zhang, Q;Cao, X;Rao, B;Xia, Y;Lu, Y;Shen, Y;Yao, Y;Xu, H;Ma, P;Cao, Y;Qin, A;
Nature communications
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Efficient and scalable generation of primordial germ cells in 2D culture using basement membrane extract overlay
Authors: Arend W. Overeem, Yolanda W. Chang, Ioannis Moustakas, Celine M. Roelse, Sanne Hillenius, Talia Van Der Helm et al.
Cell Reports Methods
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Dynamic interplay between IL-1 and WNT pathways in regulating dermal adipocyte lineage cells during skin development and wound regeneration
Authors: Sun, L;Zhang, X;Wu, S;Liu, Y;Guerrero-Juarez, CF;Liu, W;Huang, J;Yao, Q;Yin, M;Li, J;Ramos, R;Liao, Y;Wu, R;Xia, T;Zhang, X;Yang, Y;Li, F;Heng, S;Zhang, W;Yang, M;Tzeng, CM;Ji, C;Plikus, MV;Gallo, RL;Zhang, LJ;
Cell reports
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CD301b+ macrophage: the new booster for activating bone regeneration in periodontitis treatment
Authors: Can Wang, Qin Zhao, Chen Chen, Jiaojiao Li, Jing Zhang, Shuyuan Qu et al.
International Journal of Oral Science
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Multiparametric senescent cell phenotyping reveals CD24 osteolineage cells as targets of senolytic therapy in the aged murine skeleton
Authors: Madison L. Doolittle, Dominik Saul, Japneet Kaur, Jennifer L. Rowsey, Stephanie J. Vos, Kevin D. Pavelko et al.
bioRxiv
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Erythropoietin Receptor (EPOR) Signaling in the Osteoclast Lineage Contributes to EPO-Induced Bone Loss in Mice
Authors: Z Awida, S Hiram-Bab, A Bachar, H Saed, D Zyc, A Gorodov, N Ben-Califa, S Omari, J Omar, L Younis, JA Iden, L Graniewitz, I Gluzman, T Liron, B Raphael-Mi, A Kolomansky, M Rauner, B Wielockx, Y Gabet, D Neumann
International Journal of Molecular Sciences, 2022-10-10;23(19):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Hyaluronidase 1 deficiency decreases bone mineral density in mice
Authors: E Puissant, F Gilis, V Tevel, JM Vandeweerd, B Flamion, M Jadot, M Boonen
Oncogene, 2022-06-16;12(1):10142.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Orchestration of energy metabolism and osteogenesis by Mg2+ facilitates low-dose BMP-2-driven regeneration
Authors: S Lin, S Yin, J Shi, G Yang, X Wen, W Zhang, M Zhou, X Jiang
Bioactive materials, 2022-03-24;18(0):116-127.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Single-cell RNA landscape of the osteoimmunology microenvironment in periodontitis
Authors: Y Chen, H Wang, Q Yang, W Zhao, Y Chen, Q Ni, W Li, J Shi, W Zhang, L Li, Y Xu, H Zhang, D Miao, L Xing, W Sun
Theranostics, 2022-01-01;12(3):1074-1096.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
The Non-Erythropoietic EPO Analogue Cibinetide Inhibits Osteoclastogenesis In Vitro and Increases Bone Mineral Density in Mice
Authors: Z Awida, A Bachar, H Saed, A Gorodov, N Ben-Califa, M Ibrahim, A Kolomansky, JA Iden, L Graniewitz, T Liron, S Hiram-Bab, M Brines, Y Gabet, D Neumann
International Journal of Molecular Sciences, 2021-12-21;23(1):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
The m6A demethylase FTO promotes the osteogenesis of mesenchymal stem cells by downregulating PPARG
Authors: LS Chen, M Zhang, P Chen, XF Xiong, PQ Liu, HB Wang, JJ Wang, J Shen
Acta pharmacologica Sinica, 2021-08-30;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Interleukin‐4 repairs wear particle induced osteolysis by modulating macrophage polarization and bone turnover
Authors: Jukka Pajarinen, Tzuhua Lin, Akira Nabeshima, Taishi Sato, Emmanuel Gibon, Eemeli Jämsen et al.
Journal of Biomedical Materials Research Part A
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Mitochondrial TNAP controls thermogenesis by hydrolysis of phosphocreatine
Authors: Y Sun, JF Rahbani, MP Jedrychows, CL Riley, S Vidoni, D Bogoslavsk, B Hu, PA Dumesic, X Zeng, AB Wang, NH Knudsen, CR Kim, A Marasciull, JL Millán, ET Chouchani, L Kazak, BM Spiegelman
Nature, 2021-05-12;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Proteomics of protein trafficking by in vivo tissue-specific labeling
Authors: Ilia A. Droujinine, Amanda S. Meyer, Dan Wang, Namrata D. Udeshi, Yanhui Hu, David Rocco et al.
Nature Communications
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T cell-depleting nanoparticles ameliorate bone loss by reducing activated T cells and regulating the Treg/Th17 balance
Authors: X Yang, F Zhou, P Yuan, G Dou, X Liu, S Liu, X Wang, R Jin, Y Dong, J Zhou, Y Lv, Z Deng, S Liu, X Chen, Y Han, Y Jin
Bioactive materials, 2021-03-10;6(10):3150-3163.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
miRNA-92a-3p regulates osteoblast differentiation in patients with concomitant limb fractures and TBI via IBSP/PI3K-AKT inhibition
Authors: Liangcong Hu, Jing Liu, Hang Xue, Adriana C. Panayi, Xudong Xie, Ze Lin et al.
Molecular Therapy - Nucleic Acids
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BMP2-dependent gene regulatory network analysis reveals Klf4 as a novel transcription factor of osteoblast differentiation
Authors: S Yu, J Guo, Z Sun, C Lin, H Tao, Q Zhang, Y Cui, H Zuo, Y Lin, S Chen, H Liu, Z Chen
Cell Death & Disease, 2021-02-19;12(2):197.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Frizzled related protein deficiency impairs muscle strength, gait and calpain 3 levels
Authors: Leire Casas-Fraile, Frederique M. Cornelis, Domiziana Costamagna, Anabel Rico, Robin Duelen, Maurilio M. Sampaolesi et al.
Orphanet Journal of Rare Diseases
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4-phenylbutyric acid enhances the mineralization of osteogenesis imperfecta iPSC-derived osteoblasts
Authors: S Takeyari, T Kubota, Y Ohata, M Fujiwara, T Kitaoka, Y Taga, K Mizuno, K Ozono
J Biol Chem, 2020-11-23;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Exosomes released from educated mesenchymal stem cells accelerate cutaneous wound healing via promoting angiogenesis
Authors: Xinyu Qiu, Jin Liu, Chenxi Zheng, Yuting Su, Lili Bao, Bin Zhu et al.
Cell Proliferation
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Impaired autophagy triggered by HDAC9 in mesenchymal stem cells accelerates bone mass loss
Authors: L Zhang, M Qi, J Chen, J Zhao, L Li, J Hu, Y Jin, W Liu
Stem Cell Res Ther, 2020-07-03;11(1):269.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Nano-Polyplexes Mediated Transfection of Runx2-shRNA Mitigates the Osteodifferentiation of Human Valvular Interstitial Cells
Authors: G Voicu, D Rebleanu, CA Constantin, EV Fuior, L Ciortan, I Droc, CM Uritu, M Pinteala, I Manduteanu, M Simionescu, M Calin
Pharmaceutics, 2020-06-02;12(6):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Sphingosine 1-phosphate-regulated transcriptomes in heterogenous arterial and lymphatic endothelium of the aorta
Authors: Eric Engelbrecht, Michel V Levesque, Liqun He, Michael Vanlandewijck, Anja Nitzsche, Hira Niazi et al.
eLife
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A polysaccharide from the dried rhizome of Drynaria fortunei (Kunze) J. Sm. prevents ovariectomized (OVX)-induced osteoporosis in rats
Authors: X Sun, B Wei, Z Peng, X Chen, Q Fu, C Wang, J Zhen, J Sun
J. Cell. Mol. Med., 2020-02-17;0(0):.
Species: Rat
Sample Types: Serum
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Icaritin promotes the osteogenesis of bone marrow mesenchymal stem cells via the regulation of sclerostin expression
Authors: Qiushi Wei, Bin Wang, Hailan Hu, Chuhai Xie, Long Ling, Jianliang Gao et al.
International Journal of Molecular Medicine
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Optimal stimulation toward the dermal papilla lineage can be promoted by combined use of osteogenic and adipogenic inducers
Authors: T Kazi, I Niibe, A Nishikawa, T Matsuzaki
FEBS Open Bio, 2019-12-28;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
The Human Amniotic Mesenchymal Stem Cells (hAMSCs) Improve the Implant Osseointegration and Bone Regeneration in Maxillary Sinus Floor Elevation in Rabbits
Authors: L Yin, ZX Zhou, M Shen, N Chen, F Jiang, SL Wang
Stem Cells Int, 2019-12-11;2019(0):9845497.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Responses of primary osteoblasts and osteoclasts from hemizygous beta-globin knockout thalassemic mice with elevated plasma glucose to 1,25-dihydroxyvitamin D3
Authors: N Charoenpha, R Aeimlapa, S Sooksawanw, J Thongbunch, J Teerapornp, S Svasti, K Wongdee
Sci Rep, 2019-09-27;9(1):13963.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Mesenchymal Precursor Cells in Adult Nerves Contribute to Mammalian Tissue Repair and Regeneration
Authors: MJ Carr, JS Toma, APW Johnston, PE Steadman, SA Yuzwa, N Mahmud, PW Frankland, DR Kaplan, FD Miller
Cell Stem Cell, 2018-11-29;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Alkaline Phosphatase Controls Lineage Switching of Mesenchymal Stem Cells by Regulating the LRP6/GSK3? Complex in Hypophosphatasia
Authors: W Liu, L Zhang, K Xuan, C Hu, L Li, Y Zhang, F Jin, Y Jin
Theranostics, 2018-11-09;8(20):5575-5592.
Species: Human
Sample Types: Whole Cells, Whole Tissue
Applications: ICC, IHC-P -
Tissue-Nonspecific Alkaline Phosphatase Is Required for MC3T3 Osteoblast-Mediated Protection of Acute Myeloid Leukemia Cells from Apoptosis
Authors: RM Sterner, KN Kremer, A Dudakovic, JJ Westendorf, AJ van Wijnen, KE Hedin
J. Immunol., 2018-06-18;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Morphological and functional analyses of skeletal muscles from an immunodeficient animal model of limb girdle muscular dystrophy type 2E
Authors: G Giovannell, G Giacomazzi, H Grosemans, M Sampaolesi
Muscle Nerve, 2018-02-24;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Multicolor quantitative confocal imaging cytometry
Authors: DL Coutu, KD Kokkaliari, L Kunz, T Schroeder
Nat. Methods, 2017-11-13;15(1):39-46.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Cardiac Fibroblasts Adopt Osteogenic Fates and Can Be Targeted to Attenuate Pathological Heart Calcification
Authors: Arjun Deb
Cell Stem Cell, 2016-11-17;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Osteoclast-derived exosomal miR-214-3p inhibits osteoblastic bone formation
Authors: Defang Li, Jin Liu, Baosheng Guo, Chao Liang, Lei Dang, Cheng Lu et al.
Nature Communications
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Pannexin 3 and connexin 43 modulate skeletal development through their distinct functions and expression patterns
Authors: Masaki Ishikawa, Geneva L. Williams, Tomoko Ikeuchi, Kiyoshi Sakai, Satoshi Fukumoto, Yoshihiko Yamada
Journal of Cell Science
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Polarized ATP distribution in urothelial mucosal and serosal space is differentially regulated by stretch and ectonucleotidases
Authors: Weiqun Yu
American Journal of Physiology-Renal Physiology
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Dysregulation of Mitochondrial Functions and Osteogenic Differentiation in Cisd2-Deficient Murine Induced Pluripotent Stem Cells
Authors: Ping-Hsing Tsai, Yueh Chien, Jen-Hua Chuang, Shih-Jie Chou, Chian-Hsu Chien, Ying-Hsiu Lai et al.
Stem Cells and Development
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DLX3 regulates bone mass by targeting genes supporting osteoblast differentiation and mineral homeostasis in vivo.
Authors: Isaac, J, Erthal, J, Gordon, J, Duverger, O, Sun, H-W, Lichtler, A C, Stein, G S, Lian, J B, Morasso, M I
Cell Death Differ, 2014-06-20;21(9):1365-76.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Ablation of coactivator Med1 switches the cell fate of dental epithelia to that generating hair.
Authors: Yoshizaki, Keigo, Hu, Lizhi, Nguyen, Thai, Sakai, Kiyoshi, He, Bing, Fong, Chak, Yamada, Yoshihik, Bikle, Daniel D, Oda, Yuko
PLoS ONE, 2014-06-20;9(6):e99991.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Midkine-deficiency increases the anabolic response of cortical bone to mechanical loading.
Authors: Liedert A, Mattausch L, Rontgen V, Blakytny R, Vogele D, Pahl M, Bindl R, Neunaber C, Schinke T, Harroch S, Amling M, Ignatius A
Bone, 2010-12-23;48(4):945-51.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
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Mouse tibiae slices stained with ALP (AF2910) at a 1:200 dilution + Alexa Fluor donkey-antigoat 594 1:200.
DAPI (cyan)
ALPL (yellow)
Mouse bone marrow cells were stained with Goat Anti-Mouse Alkaline Phosphatase/ALPL Antigen Affinity-purified Polyclonal Antibody or followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody.