Mouse alpha 1-Acid Glycoprotein Antibody

Catalog #: AF5934 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5934
AF5934-SP

Detection of Mouse alpha 1-Acid Glycoprotein by Western Blot.

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Mouse alpha 1-Acid Glycoprotein Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse alpha 1‑Acid Glycoprotein in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with human alpha 1‑Acid Glycoprotein is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse plasma-derived alpha 1‑Acid Glycoprotein
Accession # P02763

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse alpha 1-Acid Glycoprotein by Western Blot. Western blot shows lysates of mouse liver tissue. PVDF membrane was probed with 1 µg/mL of Mouse a1-Acid Glycoprotein Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5934) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for a1-Acid Glycoprotein at approximately 43kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: alpha 1-Acid Glycoprotein

alpha 1-Acid Glycoprotein (AGP; also OMD/Orosomucoid) is a 40‑46 kDa member of the immunocalin subfamily, lipocalin family of molecules. In mouse, circulating AGP is principally the product of hepatocytes that originates from multiple related genes (AGP-1, -2 & -3 in Mus musculus). Circulating AGP-1 and -2 are both 189 amino acids (aa) in length, the principal sources of protein, and show 83% aa identity; AGP-3 contributes little to the AGP pool. In mouse blood, AGP is normally 200‑400 μg/mL. In response to inflammatory mediators (IL-6; IL-1), its concentration will rise 2 to 10 fold. More importantly, a complex glycosylation pattern will also change, transitioning from modestly branched to highly branched oligosaccharides. This change is reflected in its bioactivity, which has been shown to be a function of carbohydrate branching. AGP is generally considered to be a suppressor of inflammation. Rat and human AGP share only 70% and 47% aa identity with mouse AGP, respectively.

Entrez Gene IDs

5004 (Human); 18405 (Mouse); 24614 (Rat)

Alternate Names

AGP 1; AGP; AGP1; AGP1alpha-1-acid glycoprotein 1; AGPA; AGP-A; alpha 1Acid Glycoprotein; alpha 1-Acid Glycoprotein; OMD 1; ORM; ORM1; orosomucoid 1; Orosomucoid; orosomucoid-1