Mouse AMICA/JAML Biotinylated Antibody

Catalog #: BAF3106 Datasheet / COA / SDS

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BAF3106

All AMICA/JAML Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse AMICA/JAML Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse AMICA/JAML in Western blots. In Western blots, approximately 20% cross-reactivity with recombinant human AMICA is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse AMICA/JAML
Leu23-Leu281
Accession # Q80UL9

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse AMICA/JAML Fc Chimera (Catalog # 3106-AM)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: AMICA/JAML

AMICA, also known as JAML, is a 65 kDa, type I transmembrane glycoprotein that belongs to the junctional adhesion molecule (JAM) subset of the immunoglobulin superfamily (1). JAM family proteins contribute to intercellular connections within epithelial and endothelial cell layers and mediate their interactions with various hemopoietic cells (1). The mouse AMICA cDNA encodes a 379 amino acid (aa) precursor that includes a 20 aa signal sequence, a 261 aa extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 77 aa cytoplasmic domain (2). In contrast to other JAM family proteins, AMICA does not contain a cytoplasmic PDZ-binding motif (3). Within the ECD, mouse AMICA shares 58% and 77% aa sequence identity with human and rat AMICA, respectively. It shares 17%‑23% aa sequence identity with the ECDs of mouse JAM-A, -B, -C, and JAM4. AMICA is expressed on the surface of granulocytes and monocytes and is upregulated during the differentiation of myeloid leukemia cells (2, 3). A motif in the ECD, which promotes dimerization of other JAM family proteins, is required for surface localization of AMICA (2). AMICA mediates adhesion of monocytes to endothelial cells (2) and neutrophil migration across epithelial cell monolayers (3). This latter function involves specific interactions of AMICA with CXADR in epithelial tight junctions (3). In particular, the membrane proximal Ig-like domain of AMICA binds the membrane-distal Ig-like domain of CXADR (3). AMICA does not appear to interact homophilically, as neutrophils adhere to immobilized CXADR but not to immobilized AMICA (3).

Long Name

Adhesion Molecule, Interacts With CXADR Antigen 1

Entrez Gene IDs

120425 (Human); 270152 (Mouse)

Alternate Names

adhesion molecule, interacts with CXADR antigen 1; AMICA; AMICA1; CREA7-1; Gm638; JAML