Mouse AMIGO3 Antibody

Catalog #: MAB2375 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2375
MAB2375-SP

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Mouse AMIGO3 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse AMIGO3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant mouse AMIGO2, recombinant human (rh) AMIGO1, or rhAMIGO2 is observed.

Source

Monoclonal Rat IgG_2A Clone # 371301

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse AMIGO3
Thr20-Pro378
Accession # Q8C2S7

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse AMIGO3

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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AMIGO3 in Mouse Brain. AMIGO3 was detected in perfusion fixed frozen sections of mouse brain (cerebellum) using 25 µg/mL Rat Anti-Mouse AMIGO3 Monoclonal Antibody (Catalog # MAB2375) overnight at 4 °C. Tissue was stained (red). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: AMIGO3

AMIGO3 (Amphoterin-Induced Gene and ORF 3) is a member of the AMIGO family of type I transmembrane proteins that contain 6 leucine-rich repeats (CRRs) and one Ig domain in their extracellular domains. It is ubiquitously expressed and is detected in all tissues studied. AMIGO3 shows less than 40% amino acid sequence homology with AMIGO1 and 2. The extracellular domain of mouse AMIGO3 shares 90% and 75% amino acid sequence identity with that of rat and human AMIGO3, respectively.

Long Name

Amphoterin-induced Gene and ORF 3

Entrez Gene IDs

386724 (Human); 320844 (Mouse)

Alternate Names

adhesion molecule with Ig-like domain 3; ALI3; Alivin-3; AMIGO3; AMIGO-3; amphoterin-induced gene and ORF 3; amphoterin-induced protein 3; KIAA1851; MGC120552