Mouse Aminopeptidase PILS/ARTS1 Antibody

Catalog #: MAB2500 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2500
MAB2500-SP

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Aminopeptidase PILS/ARTS1 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Aminopeptidase PILS/ARTS1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross‑reactivity with recombinant human Aminopeptidase PILS/ARTS is observed.

Source

Monoclonal Rat IgG_2B Clone # 325908

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Aminopeptidase PILS/ARTS1
Ser27-Leu930
Accession # NP_109636

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse Aminopeptidase PILS/ARTS1 (Catalog # 2500-ZN)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Aminopeptidase PILS/ARTS1

The mouse ARTS1 gene encodes aminopeptidase PILS (Puromycin-Insensitive Leucyl-Specific), which is also known as adipocyte‑derived leucine aminopeptidase, type 1 tumor necrosis factor receptor shedding aminopeptidase regulator and ERAAP (the aminopeptidase associated with antigen processing in the endoplasmic reticulum) (1‑3). The deduced amino acid sequence of mouse ARTS1 consists of a signal peptide and a large ectodomain. Widely expressed and releasing an N‑terminal amino acid (Leu), the enzyme may play a role in many processes such as antigen processing and angiogenesis.

References

Miyashita, H. et al. (2002) Blood 99:3241.
Serwold, T. et al. (2002) Nature 419:480.
Schomburg, L. (2004) in Handbook of Proteolytic Enzymes. Barrett, et al. eds. p. 311, Academic Press, San Diego.

Long Name

Aminopeptidase Puromycin-insensitive Leucyl-specific

Entrez Gene IDs

51752 (Human); 80898 (Mouse); 102146570 (Cynomolgus Monkey)

Alternate Names

Adipocyte-derived leucine aminopeptidase; A-LAP; A-LAPALAP; Aminopeptidase PILS; ARTS1; ARTS1aminopeptidase regulator of TNFR1 shedding; ARTS-1PILSAP; EC 3.4.11; EC 3.4.11.-; EC 3.4.11.1; endoplasmic reticulum aminopeptidase 1; endoplasmic reticulum aminopeptidase associated with antigen processing; ERAAP1; ERAP1; KIAA0525APPILS; PILS-AP; PILS-APERAAP; Puromycin-insensitive leucyl-specific aminopeptidase; Type 1 tumor necrosis factor receptor shedding aminopeptidase regulator