Mouse ASAH2/N‑acylsphingosine Amidohydrolase‑2 Antibody

Catalog #: AF3558
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3558
AF3558-SP

All ASAH2/N-acylsphingosine Amidohydrolase-2 Antibodies

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Citations (1)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse ASAH2/N‑acylsphingosine Amidohydrolase‑2 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse ASAH2/N‑acylsphingosine Amidohydrolase-2 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross‑reactivity with recombinant human ASAH2 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse ASAH2/N‑acylsphingosine Amidohydrolase-2
Thr34-Thr756
Accession # Q9JHE3

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse ASAH2/N‑acylsphingosine Amidohydrolase-2 (Catalog # 3558-AH)

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Mouse ASAH2/N‑acylsphingosine Amidohydrolase-2 (Catalog # 3558-AH), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ASAH2/N-acylsphingosine Amidohydrolase-2

The mouse ASAH2 gene encodes acylsphingosine amidohydrolase-2, also known as neutral ceramidase. Neutral ceramidase is a type II integral membrane protein that can be cleaved to produce a soluble secreted protein (1). The enzyme is abundant in the brush border membranes of the intestine, but is also expressed in tissues such as kidney, brain and liver (2, 3). A major physiological function of neutral ceramidase is the metabolism of dietary sphingolipids, but the enzyme may also be involved in the generation of messenger molecules such as sphingosine and sphingosine 1-phosphate (3).

References

Tani, M. et al. (2003) J. Biol. Chem. 278:10523.
Kono, M. et al. (2006) J. Biol. Chem. 281:7324.
Mitsutake, S. et al. (2001) J. Biol. Chem. 276:26249.

Long Name

Neutral Ceramidase

Entrez Gene IDs

56624 (Human); 54447 (Mouse); 114104 (Rat)

Alternate Names

ASAH2 N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2; ASAH2; BCDase; HNAC1; LCDase; Nacylsphingosine Amidohydrolase2; NCDase; N-CDase

Product Datasheets

Product Datasheet

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Related Research Areas

Citation for Mouse ASAH2/N‑acylsphingosine Amidohydrolase‑2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1